Filtration In Gel
Mostrando 13-24 de 2525 artigos, teses e dissertações.
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13. Copper induction and differential expression of laccase in Aspergillus flavus
Aspergillus flavus was isolated from soil and exhibited laccase activity under both constitutive and copper induced conditions. Spiking the medium with 1 mM copper sulfate resulted in an increase in the activity which reached 51.84 U/mL, a distinctive protein band was detected at 60 kDa. The extracellular enzyme was purified 81 fold using gel filtration chro
Braz. J. Microbiol.. Publicado em: 2015-03
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14. ACE-I inhibitory properties of hydrolysates from germinated and ungerminated Phaseolus lunatus proteins
Phaseolus lunatus protein concentrates and the proteases Alcalase(R) and Pepsin-Pancreatin were used for the production of protein hydrolysates that inhibit angiotensin-I converting enzyme (ACE). Protein concentrate obtained from germinated and ungerminated seeds flour was hydrolyzed with Alcalase(R) at enzyme/substrate ratio (E/S) 1/10 and during 0.5 and 2.
Food Sci. Technol. Publicado em: 2015-03
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15. Purification and Characterization of a Polygalacturonase Produced by Wickerhamomyces anomalus
The aim of this work was to study the purification and physicochemical properties of an endo-polygalacturonase (PG) produced by Wickerhamomyces anomalus isolated from the citrus fruit peels. The enzyme was purified to homogeneity from the culture filtrate of W. anomalus grown on the yeast nitrogen base medium with glucose as carbon and energy source and citr
Braz. arch. biol. technol.. Publicado em: 2014-08
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16. Purification and characterization of a hyaluronidase from venom of the spider Vitalius dubius (Araneae, Theraphosidae)
BackgroundVenom hyaluronidase (Hyase) contributes to the diffusion of venom from the inoculation site. In this work, we purified and characterized Hyase from the venom of Vitalius dubius (Araneae, Theraphosidae), a large theraphosid found in southeastern Brazil. Venom obtained by electrical stimulation of adult male and female V. dubius was initially fractio
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 04/02/2014
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17. An oxidant and organic solvent tolerant alkaline lipase by P. aeruginosa mutant: downstream processing and biochemical characterization
An extracellular alkaline lipase from Pseudomonas aeruginosa mutant has been purified to homogeneity using acetone precipitation followed by anion exchange and gel filtration chromatography and resulted in 27-fold purification with 19.6% final recovery. SDS-PAGE study suggested that the purified lipase has an apparent molecular mass of 67 kDa. The optimum te
Braz. J. Microbiol.. Publicado em: 2013-12
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18. Evaluation of Antimicrobial Activity of Glucose Oxidase from Aspergillus niger EBL-A and Penicillium notatum
This work aimed to study the production and purification of glucose oxidase by Aspergillus niger and Penicillium notatum using corn steep liquor as the substrate and evaluate its antimicrobial activity for use in pharmaceutical and food industries. The enzyme was purified by ammonium sulfate precipitation (60-85%), DEAE-cellulose ion exchange and Sephadex G-
Braz. arch. biol. technol.. Publicado em: 08/11/2013
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19. Induced defense responses in tomato against bacterial spot by proteins synthesized by endophytic bacteria
Some endophytes can synthesize molecules that elicit the induction of plant resistance to infection by pathogens. The objective of this study was to demonstrate that protein fractions 42 and 75 from Bacillus amyloliquefaciens and Bacillus pumilus were capable of acting as elicitors of induced resistance in tomato plants against Xanthomonas vesicatoria, follo
Trop. plant pathol.. Publicado em: 09/07/2013
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20. Effect of castration on renal glycosaminoglycans and their urinary excretion in male and female rats with chronic renal failure
Glycosaminoglycans (GAGs) participate in a variety of processes in the kidney, and evidence suggests that gender-related hormones participate in renal function. The aim of this study was to analyze the relationship of GAGs, gender, and proteinuria in male and female rats with chronic renal failure (CRF). GAGs were analyzed in total kidney tissue and 24-h uri
Braz J Med Biol Res. Publicado em: 2013-07
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21. Leptin and insulin in the seminal plasma of zebu bulls in peripuberty
In order to determine the profile of leptin and insulin values in seminal plasma and their correlations with sperm patterns during the peripuberty, a total of 16 crossbred Gyr dairy bulls were monitored from 60 days before to 60 days after puberty, separated into two groups, early and regular, according to the onset of puberty. Hormone values were determined
Revista Brasileira de Zootecnia. Publicado em: 2012-04
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22. Biosynthesis, purification and characterization of endoglucanase from a xylanase producing strain Aspergillus niger B03
An extracellular endoglucanase was isolated from the culture liquid of xylanase producing strain Aspergillus niger B03. The enzyme was purified to a homogenous form, using consecutive ultrafiltration, anion exchange chromatography, and gel filtration. Endoglucanase was a monomer protein with a molecular weight of 26,900 Da determined by sodium dodecyl sulfat
Brazilian Journal of Microbiology. Publicado em: 2012-03
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23. Production of rabbit antibodies against purified Glucose oxidase
Glucose oxidase is an active oxygen species generating enzyme produced from Aspergillus niger grown in submerged fermentation. Disintegration of the mycelium resulted in high glucose oxidase activity that was subjected to ammonium sulfate precipitation at 60-85% saturation rates that resulted to 6.14 U mg -1 specific activity. Purification of enzyme by anion
Brazilian Archives of Biology and Technology. Publicado em: 2012-02
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24. An alternative method to isolate protease and phospholipase A2 toxins from snake venoms based on partitioning of aqueous two-phase systems
Snake venoms are rich sources of active proteins that have been employed in the diagnosis and treatment of health disorders and antivenom therapy. Developing countries demand fast economical downstream processes for the purification of this biomolecule type without requiring sophisticated equipment. We developed an alternative, simple and easy to scale-up me
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 2012