Explant
Mostrando 13-24 de 264 artigos, teses e dissertações.
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13. Genetic transformation of Brachiaria brizantha cv. Marandu by biolistics
ABSTRACT Brachiaria brizantha is a forage grass well adapted to tropical areas and cultivated in millions of hectares in Brazil. The apomictic mode of reproduction in this species, in addition to differences in ploidy between sexual and apomictic plants, impairs crossbreeding. The development of a methodology to transform apomictic cultivars will provide an
An. Acad. Bras. Ciênc.. Publicado em: 2018-04
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14. Micropropagation of Cyrtopodium paludicolum (Orchidaceae) from root tip explants
Abstract An efficient protocol for in vitro plant propagation of Cyrtopodium paludicolum has been developed using root tips dissected from well-developed seedlings. Root tips were cultured on Knudson medium supplemented with α-naphthaleneacetic acid (NAA), and/or thidiazuron (TDZ). TDZ did not induce protocorm-like bodies (PLBs) in the NAA absence, indicati
Crop Breed. Appl. Biotechnol.. Publicado em: 2017-09
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15. Tissue culture efficiency of wheat species with different genomic formulas
Abstract Ancient wheats are increasingly considered as valuable resources for genes of interest which could be analyzed and introduced into cultivated varieties by genetic engineering technologies. The first stage of biotechnological crop improvement consists of successful in vitro plant regeneration. Twelve wheat germplasms with different genomic formulas (
Crop Breed. Appl. Biotechnol.. Publicado em: 2016-12
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16. Recurrent Somatic Embryogenesis and Plantlet Regeneration in Psidium guajava L.
A simple and efficient protocol for recurrent somatic embryogenesis and plant regeneration is one of the prerequisites for genetic improvement of guava. An efficient reproducible regeneration somatic embryogenesis protocol was developed in four genotypes of Psidium guajava L. using immature zygotic embryo as starter explant. Somatic embryogenesis induction w
Braz. arch. biol. technol.. Publicado em: 06/05/2016
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17. Influence of Benzyladenine and Thidiazuron on Shoot Regeneration from Leaf and Shoot tip Explants of Sedum sarmentosum Bunge
Effects of N6-benzyladenine (BA) or thidiazuron (TDZ) on adventitious shoot regeneration and axillary shoot multiplication of Sedum sarmentosum was investigated. Leaf and shoot tip explants obtained from in vitro-grown shoots of S. sarmentosum were cultured on Murashige and Skoog (MS) medium supplemented with 0, 2.0, 4.0 or 8.0 µM BA or TDZ. Of the two cyto
Braz. arch. biol. technol.. Publicado em: 06/05/2016
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18. Micropropagation of Endangered and Decorative Species Dianthus pinifolius Sibth. et Sm.
The aim of this study was to establish an efficient protocol for the in vitro propagation of the endangered, endemic and decorative species Dianthus pinifolius Sibth. et Sm. The effects of different concentrations of 6-benzylaminopurine (BAP) and naphtalenacetic acid (NAA), and different explant types (single-node cuttings, terminal buds and shoot cuttings)
Braz. arch. biol. technol.. Publicado em: 22/03/2016
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19. Comparative Microbicidal Efficacy of Fractionated Extracts from In Vitro and In Vivo Raised Cells of Tinosporacordifolia Against MDR Pathogens
The present study was conducted to explore the hidden potential of natural products synthesized in the medicinal plant Tinosporacordifolia. This plantis prioritized by National Medicinal Plant Board, New Delhi. Leaf and inter nodal segments were inoculated on MS Medium fortified with IBA (1.0 mg/L) produced callus after four weeks. The calli were brown due t
Braz. arch. biol. technol.. Publicado em: 22/03/2016
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20. Acetosyringone, pH and temperature effects on transient genetic transformation of immature embryos of Brazilian wheat genotypes by Agrobacterium tumefaciens
Abstract Low transformation efficiency is one of the main limiting factors in the establishment of genetic transformation of wheat via Agrobacterium tumefaciens. To determine more favorable conditions for T-DNA delivery and explant regeneration after infection, this study investigated combinations of acetosyringone concentration and pH variation in the inocu
Genet. Mol. Biol.. Publicado em: 03/11/2015
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21. Prolonged viability of human organotypic skin explant in culture method (hOSEC)
Abstract BACKGROUND: Currently, the cosmetic industry is overwhelmed in keeping up with the safety assessment of the increasing number of new products entering the market. To meet such demand, research centers have explored alternative methods to animal testing and also the large number of volunteers necessary for preclinical and clinical tests. OBJECTIVES:
An. Bras. Dermatol.. Publicado em: 2015-06
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22. Somatic embryogenesis and plant regeneration in Wedelia calendulacea Less. an endangered medicinal plant
In this work, plant regeneration via somatic embryogenesis was achieved from leaf and internode derived callus of Wedelia calendulacea, an endangered medicinal plant. Primary callus was induced by culturing leaf disc and internode explant on Murashige and Skoog medium supplemented with 2.0 mg L-1 of 2,4-D under light condition. Transfer of embryogenic callus
Braz. arch. biol. technol.. Publicado em: 2014-06
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23. CCL3 and CXCL12 production in vitro by dental pulp fibroblasts from permanent and deciduous teeth stimulated by Porphyromonas gingivalis LPS
Objective: The aim of this study was to compare the production of the chemokines CCL3 and CXCL12 by cultured dental pulp fibroblasts from permanent (PDPF) and deciduous (DDPF) teeth under stimulation by Porphyromonas gingivalis LPS (PgLPS). Material and Methods: Primary culture of fibroblasts from permanent (n=3) and deciduous (n=2) teeth were established
J. Appl. Oral Sci.. Publicado em: 2013-04
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24. Establishment and characterisation of a new cell line derived from Culex quinquefasciatus (Diptera: Culicidae)
Insect cell cultures are an important biotechnological tool for basic and applied studies. The objective of this work was to establish and characterise a new cell line from Culex quinquefasciatus embryonic tissues. Embryonated eggs were taken as a source of tissue to make explants that were seeded in L-15, Grace's, Grace's/L-15, MM/VP12, Schneider's and DMEM
Memórias do Instituto Oswaldo Cruz. Publicado em: 2012-02