Exosome
Mostrando 1-12 de 54 artigos, teses e dissertações.
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1. Exosomes promote cetuximab resistance via the PTEN/Akt pathway in colon cancer cells
Cetuximab is widely used in patients with metastatic colon cancer expressing wildtype KRAS. However, acquired drug resistance limits its clinical efficacy. Exosomes are nanosized vesicles secreted by various cell types. Tumor cell-derived exosomes participate in many biological processes, including tumor invasion, metastasis, and drug resistance. In this stu
Braz J Med Biol Res. Publicado em: 13/11/2017
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2. Caracterização funcional da proteína Nop8p de Saccharomyces cerevisiae / Functional characterization of the Saccharomyces cerevisiae nucleolar protein Nop8p
A proteína nucleolar Nop8p de levedura foi identificada inicialmente através de sua interação com Nip7p e está envolvida na formação da subunidade ribossomal 60S. A depleção de Nop8p em células de levedura leva à degradação prematura dos rRNAs, porém o mecanismo bioquímico responsável por este fenótipo ainda não é conhecido. Neste trabalho
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 21/10/2011
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3. Geração de espécies reativas por exossomos plaquetários: um possível novo mecanismo de disfunção vascular na sepse / Generation of reactive oxygen species by platelet-derived exosomes: a possible novel mechanism of vascular dysfunction in sepsis
Sepsis, the bodys response to infection, is associated with high mortality rates. Why a protective mechanism turns into a deadly clinical picture is a matter of debate, and goes largely unexplained. In previous work we demonstrated that plateled derived exosomes are found in the plasma of septic patients with septic shock and can induce endothelial and vascu
Publicado em: 2009
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4. Study of the function of the protein Nop53p in Saccharomyces cerevisiae / Caracterização da função da proteína Nop53p de Saccharomyces cerevisiae
In eukaryotes, the rRNA processing depends on several factors, such as, endonucleases, exonucleases, RNA helicases, rRNA modifying enzymes and components of the snoRNPs. With the purpose of characterizing new proteins involved in pre-rRNA processing, Nop53p was identified interacting with the nucleolar protein Nop17p in a two hybrid assay. The conditional ye
Publicado em: 2007
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5. Análise estrutural e funcional de cofatores do exossomo em Saccharomyces cerevisiae e Pyrococcus / Structural and functional analysis of exosome cofactors in Saccharomyces cerevisiae and Pyrococcus
A síntese ribossomal é uma das maiores atividades em células eucarióticas. Este processo inicia-se no nucléolo e é finalizado após a exportação das subunidades 40S e 60S para o citoplasma. Três dos RNAs ribossomais de eucariotos (18S, 5.8S e 25S) são sintetizados como um transcrito primário de 35S, o qual é processado através de uma complexa e
Publicado em: 2006
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6. Yeast Exosome Mutants Accumulate 3′-Extended Polyadenylated Forms of U4 Small Nuclear RNA and Small Nucleolar RNAs
The exosome is a protein complex consisting of a variety of 3′-to-5′ exonucleases that functions both in 3′-to-5′ trimming of rRNA precursors and in 3′-to-5′ degradation of mRNA. To determine additional exosome functions, we examined the processing of a variety of RNAs, including tRNAs, small nuclear RNAs (snRNAs), small nucleolar RNAs (snoRNAs),
American Society for Microbiology.
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7. A complex prediction: three-dimensional model of the yeast exosome
We present a model of the yeast exosome based on the bacterial degradosome component polynucleotide phosphorylase (PNPase). Electron microscopy shows the exosome to resemble PNPase but with key differences likely related to the position of RNA binding domains, and to the location of domains unique to the exosome. We use various techniques to reduce the many
Oxford University Press.
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8. The exosome of Trypanosoma brucei
The yeast exosome is a complex of at least 10 essential 3′–5′ riboexonucleases which is involved in 3′-processing of many RNA species. An exosome-like complex has been found or predicted to exist in other eukaryotes but not in Escherichia coli. The unicellular parasite Trypanosoma brucei diverged very early in eukaryotic evolution. We show here that
Oxford University Press.
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9. Prediction of the Archaeal Exosome and Its Connections with the Proteasome and the Translation and Transcription Machineries by a Comparative-Genomic Approach
By comparing the gene order in the completely sequenced archaeal genomes complemented by sequence profile analysis, we predict the existence and protein composition of the archaeal counterpart of the eukaryotic exosome, a complex of RNAses, RNA-binding proteins, and helicases that mediates processing and 3′->5′ degradation of a variety of RNA species. Th
Cold Spring Harbor Laboratory Press.
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10. Degradation of ribosomal RNA precursors by the exosome
The yeast exosome is a complex of 3′→5′ exonucleases involved in RNA processing and degradation. All 11 known components of the exosome are required during 3′ end processing of the 5.8S rRNA. Here we report that depletion of each of the individual components inhibits the early pre-rRNA cleavages at sites A0, A1, A2 and A3, reducing the levels of the
Oxford University Press.
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11. Rrp47p Is an Exosome-Associated Protein Required for the 3′ Processing of Stable RNAs
Related exosome complexes of 3′→5′ exonucleases are present in the nucleus and the cytoplasm. Purification of exosome complexes from whole-cell lysates identified a Mg2+-labile factor present in substoichiometric amounts. This protein was identified as the nuclear protein Yhr081p, the homologue of human C1D, which we have designated Rrp47p (for rRNA pr
American Society for Microbiology.
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12. The Nuclear Exosome Contributes to Autogenous Control of NAB2 mRNA Levels
The RNA-processing exosome is a complex of riboexonucleases required for 3′-end formation of some noncoding RNAs and for the degradation of mRNAs in eukaryotes. The nuclear form of the exosome functions in an mRNA surveillance pathway that retains and degrades improperly processed precursor mRNAs within the nucleus. We report here that the nuclear exosome
American Society for Microbiology.