Eukaryotic Expression Vector
Mostrando 1-12 de 128 artigos, teses e dissertações.
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1. Gene transfer of Chlorella vulgaris n-3 fatty acid desaturase optimizes the fatty acid composition of human breast cancer cells
Chlorella vulgaris has the gene of n-3 fatty acid desaturase (CvFad3), which can synthesize the precursor of n-3 polyunsaturated fatty acids (PUFAs) or convert n-6 to n-3 PUFAs. The objective of the present study was to examine whether the CvFad3 gene from C. vulgaris can be functionally and efficiently expressed in human breast cancer cells and whether its
Braz J Med Biol Res. Publicado em: 2012-12
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2. Secretory TAT-peptide-mediated protein transduction of LIF receptor α-chain distal cytoplasmic motifs into human myeloid HL-60 cells
The distal cytoplasmic motifs of leukemia inhibitory factor receptor α-chain (LIFRα-CT3) can independently induce intracellular myeloid differentiation in acute myeloid leukemia (AML) cells by gene transfection; however, there are significant limitations in the potential clinical use of these motifs due to liposome-derived genetic modifications. To produce
Braz J Med Biol Res. Publicado em: 2012-10
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3. BC047440 antisense eukaryotic expression vectors inhibited HepG2 cell proliferation and suppressed xenograft tumorigenicity
The biological functions of the BC047440 gene highly expressed by hepatocellular carcinoma (HCC) are unknown. The objective of this study was to reconstruct antisense eukaryotic expression vectors of the gene for inhibiting HepG2 cell proliferation and suppressing their xenograft tumorigenicity. The full-length BC047440 cDNA was cloned from human primary HCC
Brazilian Journal of Medical and Biological Research. Publicado em: 2012-02
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4. Efeito de candidatos a supressores de silenciamento gênico viral em expressão de proteína recombinate em plantas
The aim of this work was to increase the efficiency of transient heterolog protein expression system using viral gene-silencing suppressors and Agrobacterium integrated. The use of plants as bioreactor is a promising strategy for large scale production of the heterolog protein. However, the use of genetically modified plants may lead to low protein expressio
Publicado em: 2010
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5. Proteínas quinases envolvidas na regulação do estresse em Trypanosoma. / Protein kinases involved in stress regulation in Trypanosoma.
Protozoa of the genus Trypanosoma have a complex life cycle alternating between vertebrate and invertebrate hosts. The adaptation to different environmental conditions requires rapid changes in gene expression to fill up the morphological and metabolic requirements for survival. Very little is known about the mechanisms that control these changes and the sig
Publicado em: 2010
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6. Effects of L1-ORF2 fragments on green fluorescent protein gene expression
The retrotransposon known as long interspersed nuclear element-1 (L1) is 6 kb long, although most L1s in mammalian and other eukaryotic cells are truncated. L1 contains two open reading frames, ORF1 and ORF2, that code for an RNA-binding protein and a protein with endonuclease and reverse transcriptase activities, respectively. In this work, we examined the
Genetics and Molecular Biology. Publicado em: 28/08/2009
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7. Expressão, purificação e caracterização estrutural dos fatores de transcrição bZIP SCF12 e SCF5 de cana-de-açucar / Expression, purification and structural characterization of the sugarcane bZIP transcription factors SCF12 and SCF5
The bZIP transcription factors are present in eukaryotic organisms and are involved in the regulation of gene expression and many intracellular processes. These factors bind specific DNA sequences and are able to recognize regulatory sequences of a gene promoter. The bZIPs are characterized by a conserved region rich in basic amino acid residues as well as b
Publicado em: 2008
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8. Avaliação da imunogenicidade da proteína BYCr (Boophilus York pro-Cathepsin) expressada por vetores eucariotos.
O carrapato Rhipicephalus (Boophilus) microplus é o principal ectoparasita bovino e causa importantes perdas econômicas nas criações de bovino. O controle imunológico é estudado como um método alternativo para seu controle, no entanto, uma vacina eficaz ainda necessita ser desenvolvida. A proteína BYC (Boophilus Yolk Pro-Cathepsin) é uma aspártico
Publicado em: 2008
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9. Anaplasma marginale : análise da variabilidade do gene msp1 a e avaliação imunogênica da vacina de DNA contendo genes para MSP1a, MSP1b e MSP5 em camundongos BALB/c
Anaplasma marginale is an important tick-transmitted rickettsial pathogen of cattle that invades and multiplies within erythrocytes, causing severe hemolytic anemia during acute infection. Immunization with purified outer membrane proteins (OMP) induces protection against acute A. marginale disease. Of 21 OMP described six major surface proteins (MSPs 1a, 1b
Publicado em: 2007
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10. Caracterização de um novo fator de colonização (FC O25) de Escherichia coli isoladas de colibacilose neonatal bovina / Characterization of a new colonization (CF O25) of Escherichia coli isolated from neonatal bovine colibacillosis
Infections from Escherichia coli are frequent in domestic animals, affecting mainly newborn animals in the post weaning period, being thus responsible for important economic losses in the cattle, porcine and lamb herds. The establishment of the bacterium in the host is made by colonization and it is a process mediated by proteins called adhesins that are dis
Publicado em: 2006
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11. A versatile in vivo and in vitro eukaryotic expression vector for protein engineering.
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12. Coexpression of human immunodeficiency virus envelope proteins and tat from a single simian virus 40 late replacement vector.
A Sal I-Xho I fragment containing the genes encoding tat, art, and the envelope proteins from the BH10 clone of human immunodeficiency virus (HIV) was inserted into a simian virus 40 (SV40)-based eukaryotic expression vector. The vector is a shuttle vector that replicates to high copy numbers in both Escherichia coli and eukaryotic cells permissive for SV40