Escherichi Coli
Mostrando 1-7 de 7 artigos, teses e dissertações.
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1. Estudo da Atividade Tóxica para Aedes aegypti das proteínas Cry4Aa e Cry4Ba de Bacillus thuringiensis expressas em baculovírus recombinantes
The cry4Aa and cry4Ba genes from Brazilian strains of Bacillus thuringiensis, S-1806 and S-1989, respectively, were amplified by PCR, cloned into a plasmid cloning vector and sequenced. Sequence analysis of the cry4Aa gene showed high identity to previous known cry genes and it was cloned into both pSynXIVVI+X3 and pFastBac1 transfer vectors for expression a
Publicado em: 2007
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2. Sorotipos e marcadores de virulência de cepas de Escherichia coli produtoras de toxina shiga (STEC) isoladas de bezerros da região norte do estado do Paraná
Selecionamos para este estudo 86 cepas de Escherichia coli isoladas de 48 bezerros, 24 não-diarréicos e 24 diarréicos. Estas cepas foram examinadas quanto à presença de marcadores de virulência por PCR: stx1, stx2, ehly, eaeA, saa, lpfO113, k99, f41, clpG, f17, sta, lt-II, cnf e astA. Todos os isolados foram sorotipados e examinados quanto à expressã
Publicado em: 2006
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3. Genetic mapping of the F plasmid gene that promotes degradation of stable ribonucleic acid in Escherichia coli.
F+ Escherichi coli cells that contain an srnA mutant allele degrade their stable ribonucleic acid (RNA) extensively after RNA synthesis is blocked at 42 degrees C. The relevant gene promoting degradation of stable RNA, srnB+, or its promoter was mapped between 1.7 and 2.8 kilobases on the F plasmid by using deleted F' plasmids and chimeric plasmids composed
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4. Inhibition of Thiamine Pyrophosphate Utilization by Thiamine or Its Monophosphate in Escherichia coli
The growth of a thiamine pyrophosphate auxotroph of Escherichi coli was inhibited by either thiamine or thiamine monophosphate, and the growth of a thiamine monophosphate auxotroph was inhibited by thiamine. The thiamine pyrophosphate-dependent oxidation of pyruvate was inhibited by thiamine with whole cells of the thiamine pyrophosphate auxotroph but not wi
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5. Conservation and variation of nucleotide sequences within related bacterial genomes: Escherichia coli strains.
Changes in the patterns produced by annealing restriction endonuclease digests of bacterial genomes with probe deoxyribonucleic acids (DNAs) containing small portions of a bacterial genome provide sensitive indicator of the degree of nucleotide sequence relatedness that exists in localized regions of the genomes of closely related bacteria. We have used five
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6. Role of the host cell in bacteriophage T4 development. II. Characterization of host mutants that have pleiotropic effects on T4 growth.
Mutant host-defective Escherichi coli that fail to propagate bacteriophage T4 and have a pleiotropic effect on T4 development have been isolated and characterized. In phage-infected mutant cells, specific early phage proteins are absent or reduced in amount, phage DNA synthesis is depressed by about 50%, specific structural phage proteins, including some tai
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7. Inhibition of Host Protein Synthesis During Infection of Escherichi coli by Bacteriophage T4: III. Inhibition by Ghosts
Deoxyribonucleic acid (DNA)-less T2 “ghosts” were prepared by osmotic shock and purified by KBr density gradient centrifugation. Escherichia coli B was treated with these ghosts in inorganic salts-glycerol medium to see which features of phage infection could be elicited by ghosts. At a multiplicity that was just sufficient to block induction of β-galac