Enzyme Thrombin Like
Mostrando 1-12 de 34 artigos, teses e dissertações.
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1. Unique heterologous fibrin biopolymer with hemostatic, adhesive, sealant, scaffold and drug delivery properties: a systematic review
Abstract Fibrin biopolymers, previously referred as “fibrin glue” or “fibrin sealants”, are natural biomaterials with diverse applications on health. They have hemostatic, adhesive, sealant, scaffold and drug delivery properties and have become widely used in medical and dental procedures. Historically, these biomaterials are produced from human fibr
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 11/11/2019
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2. Subproteome of Lachesis muta rhombeata venom and preliminary studies on LmrSP-4, a novel snake venom serine proteinase
Abstract Background: Lachesis muta rhombeata is one of the venomous snakes of medical importance in Brazil whose envenoming is characterized by local and systemic effects which may produce even shock and death. Its venom is mainly comprised of serine and metalloproteinases, phospholipases A2 and bradykinin-potentiating peptides. Based on a previously report
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 15/04/2019
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3. Functional and biological insights of rCollinein-1, a recombinant serine protease from Crotalus durissus collilineatus
ABSTRACT Background: The prevalent class of snake venom serine proteases (SVSP) in Viperidae venoms is the thrombin-like enzymes, which, similarly to human thrombin, convert fibrinogen into insoluble fibrin monomers. However, thrombin-like serine proteases differ from thrombin by being unable to activate factor XIII, thus leading to the formation of loose c
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 08/04/2019
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4. Heterologous fibrin sealant derived from snake venom: from bench to bedside – an overview
Abstract Hemostatic and adhesive agents date back to World War II, when homologous fibrin sealant came onto scene. Considering that infectious diseases can be transmitted via human blood, a new heterologous fibrin sealant was standardized in the 1990s. Its components were a serine protease (a thrombin-like enzyme) extracted from the venom of Crotalus durissu
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 06/07/2017
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5. Multiple uses of fibrin sealant for nervous system treatment following injury and disease
Abstract Lesions to the nervous system often produce hemorrhage and tissue loss that are difficult, if not impossible, to repair. Therefore, scar formation, inflammation and cavitation take place, expanding the lesion epicenter. This significantly worsens the patient conditions and impairment, increasing neuronal loss and glial reaction, which in turn furthe
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 27/04/2017
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6. Purificação e caracterização biológica de uma nova serinoprotease com atividade trombina"like" do veneno total de Brothrops andianus (TLBan) / Purification and biological characterization of a new serine protease with thrombin "like" activity the whole venom of Brothrops andianus (TLBan)
In this word, a new serine protease with thrombin "like" activity the venom of Bothrops andianus (TLBan), snake of the Andes of Peru, was isolated by two steps: molecular exclusion chromatography G-75 and liquid chromatography in reversed-phase HPLC; with a high degree of purity and molecular homegenidade. Through of electrophoresis on SDS-PAGE shows the TLB
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 27/02/2012
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7. Cross-neutralization of the coagulant activity of Crotalus durissus terrificus venom from the northeast of Argentina by bivalent bothropic antivenom
Cross-neutralization of Crotalus durissus terrificus venom coagulant activity was tested using bivalent horse antivenom against Bothrops alternatus and Bothrops diporus venoms. Our in vitro and in vivo experiments showed that bothropic antivenom neutralizes the thrombin-like activity of crotalic snake venom and this cross-reaction was demonstrated by immunoa
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2012
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8. Biochemical and biological evaluation of gyroxin isolated from Crotalus durissus terrificus venom
Gyroxin, a thrombin-like enzyme isolated from Crotalus durissus terrificus venom and capable of converting fibrinogen into fibrin, presents coagulant and neurotoxic activities. The aim of the present study was to evaluate such coagulant and toxic properties. Gyroxin was isolated using only two chromatographic steps - namely gel filtration (Sephadex G-75) and
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2011
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9. Enzymatic and toxinological activities of Hypnale hypnale (hump-nosed pit viper) venom and its fractionation by ion exchange high performance liquid chromatography
Hypnale hypnale (hump-nosed pit viper) has been recently identified as one of the medically important venomous snakes in Sri Lanka and on the southwestern coast of India. The characterization of its venom is essential for understanding the pathophysiology of envenomation and for optimizing its management. In the present study, the biological properties of Hy
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2011
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10. Serum kinetics of Calloselasma rhodostoma (Malayan pit viper) venom components in a rabbit
The serum kinetics of Calloselasma rhodostoma (Malayan pit viper) venom - specifically two of its components, the major hemorrhagin (rhodostoxin) and a thrombin-like enzyme - was examined in a rabbit by double-sandwich enzyme-linked immunosorbent assay (ELISA). The animal received intramuscularly a 1.0-mg/kg dose of C. rhodostoma venom. The venom level in se
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2009
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11. Purification and partial characterization of a coagulant serine protease from the venom of the Iranian snake Agkistrodon halys
Agkistrodon halys is one of several dangerous snake species in Iran. Among the most important signs and symptoms in patients envenomated by this snake is disseminated intravascular coagulation. A thrombin-like enzyme, called AH143, was isolated from Agkistrodon halys venom by gel filtration on a Sephadex G-50 column, ion-exchange chromatography on a DEAE-Sep
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2009
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12. Fibrin adhesive derived from snake venom in periodontal surgery: histologicalanalysis
BACKGROUND: A new fibrin adhesive made of buffalo plasma-derived fibrinogen and a thrombin-like snake venom enzyme, has been successfully used to immobilize free gingival grafts. This case series histologically compared sutured grafts (control group) with others immobilized by using the fibrin adhesive (experimental group). CASE DESCRIPTION: The grafts were
Journal of Applied Oral Science. Publicado em: 2008-10