Enzyme Catalysis
Mostrando 1-12 de 558 artigos, teses e dissertações.
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1. LIPASE - CATALYZED FORMATION OF PENTYL NONANOATE USING SCREENED IMMOBILIZED LIPASE FROM Rhizomucor meihei
Abstract Bio-catalysis has attracted the special attention of industrial flavour producers in the production of valuable ester compounds. In this study, the synthesis of pentyl nonanoate ester (a short chain ester with fruity aroma) was carried out with a commercial immobilized lipase from Rhizomucor meihei Lipozyme (RMIM) as biocatalyst in the esterificatio
Braz. J. Chem. Eng.. Publicado em: 09/12/2019
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2. On-Flow Ligand Screening Assay Based on Immobilized Nucleoside Diphosphate Kinase B from Homo sapiens
We describe an on-flow zonal affinity-based chromatography assay to screen ligands for the nucleoside diphosphate kinase B enzyme (NME2) from Homo sapiens. For the first time, we have covalently immobilized NME2 on the surface of an open fused silica capillary reactor (NME2-ICER) and placed the reactor before the analytical column, which resulted in a two-di
J. Braz. Chem. Soc.. Publicado em: 24/10/2019
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3. Current applications and different approaches for microbial L-asparaginase production
ABSTRACT L-asparaginase (EC 3.5.1.1) is an enzyme that catalysis mainly the asparagine hydrolysis in L-aspartic acid and ammonium. This enzyme is presented in different organisms, such as microorganisms, vegetal, and some animals, including certain rodent's serum, but not unveiled in humans. It can be used as important chemotherapeutic agent for the treatmen
Braz. J. Microbiol.. Publicado em: 2016-12
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4. Multifunctional Imidazole-Derived Polymeric Catalyst for Dephosphorylation Reactions Potentiated by Cationic and Anionic Micelles
Results confirm the catalytic potential of imidazole groups anchored on the polymeric backbone of polyvinylimidazole (PVI) in dephosphorylation reactions with di- and tri-phosphoesters, promoting impressive enhancements up to 107-fold. We propose the imidazole groups on PVI react by nucleophilic catalysis (neutral specie) with a phosphate triester and bifunc
J. Braz. Chem. Soc.. Publicado em: 2016-02
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5. Bifunctional Porous SiO2 Complex Nanoparticles with Properties of Enzymatic Catalysis and Optical Oxygen Sensing
The porous fluorescent SiO2 nanoparticles encapsulating photosensitizer Ru(bpy)3Cl2were prepared by Stöber method and used as a carrier to immobilize glucose oxidase (GOD) to form the bifunctional porous SiO2 complex nanoparticles (BPSCNp) with the properties of enzymatic catalysis and optical oxygen sensing. The optimal immobilization conditions are as fol
J. Braz. Chem. Soc.. Publicado em: 2015-09
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6. Comparison of HMOX1 expression and enzyme activity in blue-shelled chickens and brown-shelled chickens
Blue egg coloring is attributed to biliverdin derived from the oxidative degradation of heme through catalysis by heme oxygenase (HO). The pigment is secreted into the eggshell by the shell gland. There is uncertainty as to whether the pigment is synthesized in the shell gland or in other tissues. To investigate the site of pigment biosynthesis, the expressi
Genet. Mol. Biol.. Publicado em: 2013
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7. Screening and isolation of halophilic bacteria producing industrially important enzymes
Halophiles are excellent sources of enzymes that are not only salt stable but also can withstand and carry out reactions efficiently under extreme conditions. The aim of the study was to isolate and study the diversity among halophilic bacteria producing enzymes of industrial value. Screening of halophiles from various saline habitats of India led to isolati
Braz. J. Microbiol.. Publicado em: 2012-12
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8. Produção de polímeros derivados de fontes renováveis via catálise enzimática / Production of polymers derived from renewable sources by enzyme catalysis
A busca por materiais derivados de fontes renováveis e com características como biocompatibilidade e biodegradabilidade tem crescido significativamente nos últimos anos. A utilização de enzimas na polimerização representa um grande passo para a obtenção destes, visto que possibilitam a produção de polímeros evitando a utilização de catalisadore
Publicado em: 2009
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9. Comparative study of the catalytic activity and proteinic expression of cytochrome P4501A (CYP1A) in suckermouth armored catfishes (Loricariidae) and tilapias (Cichlidae) / Estudo comparativo da atividade catalítica e expressão protéica do citocromo P4501A (CYP1A) em cascudos (Loricariidae) e tilápias (Cichlidae)
The cytochrome P450 (CYP) superfamily plays important roles in the biotransformation of xenobiotic and endogenous compounds. The CYP1A subfamily is well conserved in vertebrates and has been found in all jawed fish studied to date. In a previous study, we had found that suckermouth armored catfishes of the Loricariidae family (Hyposthomus luetkeni and H.affi
Publicado em: 2008
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10. Metalloporphyrins and salen complexes as a P450 biomimetic model for the metabolism of antiepileptic and antidepressant drugs / Metaloporfirinas e compostos salen como modelos biomiméticos do citocromo P450 no metabolismo de fármacos anticonvulsivante e antidepressivo
In this work, the catalytic activities of metalloporphyrins and the salen complex (Jacobsen catalyst) in solution and immobilized on different supports were studied in the oxidation of hydrocarbons, anticonvulsivant drugs (carbamazepine and primidone) and antidepressives (fluoxetine) by the following oxygen donors: hydrogen peroxide, terc-butyl hydroperoxide
Publicado em: 2008
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11. Modulation in pharmacological action of botropics and crotalics PLA2 in presence of an lectin isolated from marine alga Bryothammion triquetrum / Modulação da ação farmacologica de PLA2 botropicas e crotalicas em presença de uma lectina isolada da alga marinha Bryothamnion triquetrum
Lectins are proteins that bind specifically and reversibly to carbohydrates. They are widely distributed among living organisms and show many activities of biological and medical interest. In this work, two isoforms of lectins isolated from the red marine alga Bryothamnion triquetrum were purified by ion exchange followed by reverse phase chromatographies. T
Publicado em: 2007
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12. FUNÇÃO DO METAL BIVALENTE NA RNA TRIFOSFATASE DE TRYPANOSOMA CRUZI. / The role of the divalente metal in the RNA triphosphatase from Trypanosoma cruzi.
Cap triphosphatases act in the first step of the mRNA capping process, removing the -phosphoryl group from the 5 end of nascent RNA. Enzymes from trypanosomatids and other lower eukaryotes depend on metals for this catalysis. This contrasts with the cysteine-dependent activity of the corresponding enzymes of mammals. This difference points to these enzymes a
Publicado em: 2006