Enzymatic Stability
Mostrando 13-24 de 252 artigos, teses e dissertações.
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13. Bifunctional Porous SiO2 Complex Nanoparticles with Properties of Enzymatic Catalysis and Optical Oxygen Sensing
The porous fluorescent SiO2 nanoparticles encapsulating photosensitizer Ru(bpy)3Cl2were prepared by Stöber method and used as a carrier to immobilize glucose oxidase (GOD) to form the bifunctional porous SiO2 complex nanoparticles (BPSCNp) with the properties of enzymatic catalysis and optical oxygen sensing. The optimal immobilization conditions are as fol
J. Braz. Chem. Soc.. Publicado em: 2015-09
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14. Stability of immobilized Rhizomucor miehei lipase for the synthesis of pentyl octanoate in a continuous packed bed bioreactor
The enzymatic synthesis of organic compounds in continuous bioreactors is an efficient way to obtain industrially important chemicals. However, few works have focused on the study of the operational conditions and the bioprocess performance. In this work, the aliphatic ester pentyl octanoate was obtained by direct esterification using a continuous packed bed
Braz. J. Chem. Eng.. Publicado em: 2014-09
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15. Immobilization of Lipases Produced by the Endophytic Fungus Cercospora kikuchii on Chitosan Microparticles
This work studied the immobilization of Cercospora kikuchii lipases on chitosan microparticles by chemical attachment on chitosan acetate microparticles activated by glutaraldehyde (CAM) added before or after the enzyme and physical adsorption on highly deacetylated chitosan hydrochloride microparticles (CHM). Lipases covalently immobilized on pre-activated
Braz. arch. biol. technol.. Publicado em: 2014-08
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16. Peptidase with Keratinolytic Activity Secreted by Aspergillus terreus During Solid-State Fermentation
The aim of this study was to evaluate peptidase production by Aspergillus terreus in solid-state bioprocess and evaluate its parameters. The best conditions were 5.0 g of wheat bran as substrate, incubation temperature 30°C, inoculum 2.0x105spores/g and 75% saline volume, with production reaching 677 U/mL (5400 U/g culture medium) after 72 h of fermentation
Braz. arch. biol. technol.. Publicado em: 2014-08
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17. Inmovilización de enzimas lignocelulolíticas en nanopartículas magnéticas
Due to the need for more efficient, economical and environmentally-friendly technological processes, the use of enzymes has increased. However, reuse of enzymatic hydrolytic complex is required. The immobilization of enzymes provides a basis for stability and allows their reuse reflected in aspects of economic feasibility. Magnetic nanoparticles are a promis
Quím. Nova. Publicado em: 2014-06
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18. Immobilization of puerarin glycosidase from Microbacterium oxydans CGMCC 1788 increases puerarin transformation efficiency
For immobilization of puerarin glycosidase from Microbacterium oxydans CGMCC 1788 on DEAE-52 cellulose, the optimal amount of enzyme protein was 12 mg protein: 1 g DEAE-52 cellulose; the optimal pH was 6.5; and the optimal immobilization time was 6 hr. The specific activity of immobilized enzyme was 36.67 mU.g-1 carrier with an immobilization yield of 98.87%
Braz. J. Chem. Eng.. Publicado em: 2014-06
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19. Glicólise do poli(3-hidroxibutirato) por via enzimática
Poly(3-hydroxybutyrate), PHB, is a polymer with broad potential applications because of its biodegradability and biocompatibility. However, its high crystallinity is a limiting factor for many applications. To overcome this drawback, one strategy currently employed involves the reduction of the molecular weight of PHB with the concomitant formation of end-fu
Quím. Nova. Publicado em: 2014-06
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20. Extracellular proteases of Halobacillus blutaparonensis strain M9, a new moderately halophilic bacterium
Halophilic microorganisms are source of potential hydrolytic enzymes to be used in industrial and/or biotechnological processes. In the present study, we have investigated the ability of the moderately halophilic bacterium Halobacillus blutaparonensis (strain M9), a novel species described by our group, to release proteolytic enzymes. This bacterial strain a
Braz. J. Microbiol.. Publicado em: 25/02/2014
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21. Concentration, characterization and application of lipases from Sporidiobolus pararoseus strain
Lipases produced by a newly isolated Sporidiobolus pararoseus strain have potential catalytic ability for esterification reactions. After production, the enzymatic extracts (conventional crude and precipitated, 'CC' and 'CP', and industrial crude and precipitated, 'IC' e 'IP') were partially characterized. The enzymes presented, in general, higher specificit
Braz. J. Microbiol.. Publicado em: 2014
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22. Microbiological profile and aerobic stability of Tifton 85 bermudagrass silage with different additives
The objective of this study was to evaluate the microbiological profile and aerobic stability of silage with Tifton 85 bermudagrass and different additives and wilting. The studied treatments were: pre-drying in the sun for two hours before ensiling; use of bacterial-enzymatic inoculant; addition of soybean peel; addition of corn grits and use of salt in the
R. Bras. Zootec.. Publicado em: 2013-06
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23. Biochemical properties of Bacillus sp. ITP-001 lipase immobilized with a sol gel process
This work presents biochemical characterization of a lipase from a new strain of Bacillus sp. ITP-001, immobilized using a sol gel process (IB). The results from the biochemical characterization of IB showed increased activity for hydrolysis, with 526.63 U g-1 at pH 5.0 and 80 ºC, and thermal stability at 37 ºC. Enzymatic activity was stimulated by ions su
Quím. Nova. Publicado em: 2013
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24. Fatos e tendências da biocatálise
Brazil is renowned for its biodiversity; however, its economy is based on exotic plants, extraction and unsustainable use of natural resources. This issue was addressed in a recent QN review entitled "Chemistry without Borders." In order to explore the potential of Brazilian biodiversity fully, sustainable development is required in key technological areas,
Quím. Nova. Publicado em: 2013