Enzymatic Solution
Mostrando 1-12 de 218 artigos, teses e dissertações.
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1. Comparative Study of Degradation of Reactive Dyes and Decolorization and Detoxification in Aqueous Solution Applying DyP Peroxidases Isolated from Saccharomonospora viridis (SviDyP) and Thermobifida fusca (TfuDyp)
The wastewater that originates from the widespread usage of synthetic dyes in the industry have become a severe environmental problem. Several efforts have been made to develop new types of treatment which are capable of performing the degradation of the dyes from the environment. Within this scope, much attention has been drawn to enzymatic approaches, main
J. Braz. Chem. Soc.. Publicado em: 2021-06
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2. OTIMIZAÇÃO E VALIDAÇÃO DE MÉTODO ENZIMÁTICO SEMIQUANTITATIVO SIMPLES E DE BAIXO CUSTO PARA A BUSCA DE INIBIDORES DE CISTEINO PROTEASES
Cysteine proteases are an important class of enzymes involved in several essential biological processes. For this reason, these enzymes are important therapeutic targets, and therefore can be used in enzymatic assays for the search of hits. The most widely used methods in the search of enzyme inhibitors are spectrophotometric, however they have relatively hi
Quím. Nova. Publicado em: 2021-04
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3. Cocoa Agro-Industrial Residue (Theobroma cacao) as Inducer of the Production of Fungal Laccase and Kojic Acid for Application in the Biodegradation of 17-α-Ethinylestradiol
Due to the ability to catalyze the oxidation of several substrates, the enzyme laccase has been used in several applications, among them the bioremediation of emerging pollutants. This work evaluate the capacity of the cocoa agro-industrial residue to induce the production of Pycnoporus sanguineus laccase and to apply the enzymatic extract in the biodegradat
J. Braz. Chem. Soc.. Publicado em: 2020-10
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4. A Simple Approach to Analyze Sugar Nectar Composition in Flowers Using Capillary Electrophoresis and Enzymatic Assays
Despite the obvious importance of nectar, the composition of this solution remains surprisingly understudied to most of the flowers. Here we describe a simple, low-cost, and reliable methodology to analyze the three main sugars present in 210 nectar samples of Christmas Bellflowers using capillary electrophoresis (CE) and enzymatic assays. CE separation (-16
J. Braz. Chem. Soc.. Publicado em: 2020-10
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5. Imobilização enzimática: conceito e efeitos na proteólise
In recent years, great advances have been observed in the proteomic area regarding the different approaches adopted as top-down, middle-down and bottom-up. Regarding middle-down and bottom-up approaches, a previous step for protein digestion is necessary for further analysis of the generated peptides. Classical digestion protocols employing proteases are usu
Quím. Nova. Publicado em: 2020-04
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6. STABILIZATION STUDY OF TETRAMERIC Kluyveromyces lactis β-GALACTOSIDASE BY IMMOBILIZATION ON IMMOBEAD: THERMAL, PHYSICO-CHEMICAL, TEXTURAL AND CATALYTIC PROPERTIES
Abstract We investigated the immobilization of a tetrameric Kluyveromyces lactis β-galactosidase (EC: 3.2.1.23) (KL-Gal) on Immobead 150 using different support modification strategies. Immobead support was modified using an acid solution of H2SO4:HNO3 (3:1) (Immobead-Ac) or 5 % (v/v) glutaraldehyde (Immobead-Glu). Its unmodified form (Immobead) was also te
Braz. J. Chem. Eng.. Publicado em: 13/01/2020
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7. Enzymatic detergent reuse in gastroscope processing: a potential source of microorganism transmission
Objetivo: avaliar a potencial contaminação do detergente enzimático a partir de seu reuso e identificar o perfil microbiológico na solução utilizada na limpeza de aparelhos endoscópicos gastrointestinais. Método: estudo transversal realizado a partir da análise microbiológica de 76 alíquotas de 19 diferentes soluções de detergente enzimático
Rev. Latino-Am. Enfermagem. Publicado em: 05/12/2019
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8. Study of the parameters used in the encapsulation of commercial pectinase in calcium alginate and its effect on its catalytic activity
Abstract Despite the high catalytic properties of pectinases, the utilization of free enzymes always presents some hindrances such as low stability, a difficulty for product recovery and the impossibility of continuous use. Enzyme encapsulation is one of the methods used to overcome these limitations; however, some kind of effect is expected to occur on its
Food Sci. Technol. Publicado em: 31/01/2019
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9. Kinetic investigations and stability studies of two Bothrops L-amino acid oxidases
Abstract Background: L-amino acid oxidases isolated from snake venoms (SV-LAAOs) are enzymes that have great therapeutic potential and are currently being investigated as tools for developing new strategies to treat various diseases, including cancer and bacterial infections. The main objective of this study was to make a brief evaluation of the enzymatic s
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 24/01/2019
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10. Oxidative Damage and Antioxidant Response in Chenopodium murale L. Exposed to Elevated Levels of Zn
ABSTRACT Present study was carried out to investigate the effects of different Zn concentrations on growth and some antioxidant responses in Chenopodium murale L. The seeds were sown on the pots. 45-day plants were transferred to hydroponic solution containing 0.2 (control), 150, 300 and 600 µM Zn. The plants were harvested at 6 days after applying Zn treat
Braz. arch. biol. technol.. Publicado em: 29/10/2018
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11. Synergistic Effect of Adsorption and Enzymatic Conversion in the Bisphenol-A Removal by Laccase Immobilized on Poly(glycidyl methacrylate-co-ethyleneglycol dimethacrylate)
Carriers with different functional groups (epoxy, aldehyde, ethyldiazonium, phenyldiazonium and amino) were tested for immobilization of T. versicolor laccase on GMA-co-EGDMA (glycidyl methacrylate-co-ethyleneglycol dimethacrylate) microspheres. Laccase immobilized on GMA-co-EGDMA containing the phenyldiazonium functional group showed the highest activity (9
J. Braz. Chem. Soc.. Publicado em: 2017-11
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12. Omission and Resupply of Nitrogen Affect Physiological and Enzymatic Activities and the Gene Expression of Eucalypt Clones
ABSTRACT: The mineral nutrient uptake of plants in the field occurs in pulses, due to variations in the substance concentrations at the root surface. The fluctuations in nutrient supply probably induce changes in the plant, which are to date unknown for Eucalyptus. This study evaluated these changes in plant growth, nutritional status, photosynthesis, and ge
Rev. Bras. Ciênc. Solo. Publicado em: 15/12/2016