Enzymatic Immobilization
Mostrando 1-12 de 54 artigos, teses e dissertações.
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1. Imobilização enzimática: conceito e efeitos na proteólise
In recent years, great advances have been observed in the proteomic area regarding the different approaches adopted as top-down, middle-down and bottom-up. Regarding middle-down and bottom-up approaches, a previous step for protein digestion is necessary for further analysis of the generated peptides. Classical digestion protocols employing proteases are usu
Quím. Nova. Publicado em: 2020-04
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2. STABILIZATION STUDY OF TETRAMERIC Kluyveromyces lactis β-GALACTOSIDASE BY IMMOBILIZATION ON IMMOBEAD: THERMAL, PHYSICO-CHEMICAL, TEXTURAL AND CATALYTIC PROPERTIES
Abstract We investigated the immobilization of a tetrameric Kluyveromyces lactis β-galactosidase (EC: 3.2.1.23) (KL-Gal) on Immobead 150 using different support modification strategies. Immobead support was modified using an acid solution of H2SO4:HNO3 (3:1) (Immobead-Ac) or 5 % (v/v) glutaraldehyde (Immobead-Glu). Its unmodified form (Immobead) was also te
Braz. J. Chem. Eng.. Publicado em: 13/01/2020
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3. DESIGN OF IMMOBILIZED ENZYME BIOCATALYSTS: DRAWBACKS AND OPPORTUNITIES
The enzymatic processes are increasingly highlights, especially in the synthesis of chemical products with high added value. The enzyme immobilization can improve industrial biocatalytic processes. The immobilization of enzymes provides the production of efficient, stable biocatalysts, possibility of reuse and easy purification of the products, when compared
Quím. Nova. Publicado em: 26/08/2019
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4. Study of the parameters used in the encapsulation of commercial pectinase in calcium alginate and its effect on its catalytic activity
Abstract Despite the high catalytic properties of pectinases, the utilization of free enzymes always presents some hindrances such as low stability, a difficulty for product recovery and the impossibility of continuous use. Enzyme encapsulation is one of the methods used to overcome these limitations; however, some kind of effect is expected to occur on its
Food Sci. Technol. Publicado em: 31/01/2019
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5. Synergistic Effect of Adsorption and Enzymatic Conversion in the Bisphenol-A Removal by Laccase Immobilized on Poly(glycidyl methacrylate-co-ethyleneglycol dimethacrylate)
Carriers with different functional groups (epoxy, aldehyde, ethyldiazonium, phenyldiazonium and amino) were tested for immobilization of T. versicolor laccase on GMA-co-EGDMA (glycidyl methacrylate-co-ethyleneglycol dimethacrylate) microspheres. Laccase immobilized on GMA-co-EGDMA containing the phenyldiazonium functional group showed the highest activity (9
J. Braz. Chem. Soc.. Publicado em: 2017-11
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6. Carbon dioxide/methanol conversion cycle based on cascade enzymatic reactions supported on superparamagnetic nanoparticles
ABSTRACT The conversion of carbon dioxide into important industrial feedstock is a subject of growing interest in modern society. A possible way to achieve this goal is by carrying out the CO2/methanol cascade reaction, allowing the recycle of CO2 using either chemical catalysts or enzymes. Efficient and selective reactions can be performed by enzymes; howev
An. Acad. Bras. Ciênc.. Publicado em: 16/10/2017
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7. BIOSYNTHESIS OF INDUSTRIAL ENZYMES BY FREE AND IMMOBILIZED Alicyclobacillus spp IN DIFFERENT MATRICES AND THE USE OF ULTRAFILTRATION IN THE ENZYMES CONCENTRATION
The biosynthesis of amylase and collagenase, produced by A. acidocaldarius and A. sendaiensis respectively, were studied, and different matrices evaluated for the microorganisms immobilization and enzymes production optimization, such as loofa sponge, alginate-sponge and alginate, followed by concentration through ultrafiltration. Using a wheat bran substrat
Quím. Nova. Publicado em: 2017-09
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8. Methacrylated Chitosan Based UV Curable Support for Enzyme Immobilization
UV curing is simple, fast and effective procedure for enzyme immobilization with minimized enzymatic activity. UV-curable methacrylated chitosan is here proposed as a support material for immobilization of lipase. The morphology of the polymeric support was characterized by scanning electron microscopy (SEM). Both covalently (CIL) and physically (PIL) immobi
Mat. Res.. Publicado em: 16/02/2017
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9. Synthesis of structured triacylglycerols enriched in n-3 fatty acids by immobilized microbial lipase
Abstract The search for new biocatalysts has aroused great interest due to the variety of micro-organisms and their role as enzyme producers. Native lipases from Aspergillus niger and Rhizopus javanicus were used to enrich the n-3 long-chain polyunsaturated fatty acids content in the triacylglycerols of soybean oil by acidolysis with free fatty acids from sa
Braz. J. Microbiol.. Publicado em: 2016-12
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10. Cellulase activity and dissolved organic carbon release from lignocellulose macrophyte-derived in four trophic conditions
Abstract Considering the importance of lignocellulose macrophyte-derived for the energy flux in aquatic ecosystems and the nutrient concentrations as a function of force which influences the decomposition process, this study aims to relate the enzymatic activity and lignocellulose hydrolysis in different trophic statuses. Water samples and two macrophyte spe
Braz. J. Microbiol.. Publicado em: 2016-06
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11. Bifunctional Porous SiO2 Complex Nanoparticles with Properties of Enzymatic Catalysis and Optical Oxygen Sensing
The porous fluorescent SiO2 nanoparticles encapsulating photosensitizer Ru(bpy)3Cl2were prepared by Stöber method and used as a carrier to immobilize glucose oxidase (GOD) to form the bifunctional porous SiO2 complex nanoparticles (BPSCNp) with the properties of enzymatic catalysis and optical oxygen sensing. The optimal immobilization conditions are as fol
J. Braz. Chem. Soc.. Publicado em: 2015-09
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12. PERFORMANCE OF LOOFA-IMMOBILIZED Rhizopus oryzae IN THE ENZYMATIC PRODUCTION OF BIODIESEL WITH USE OF OLEIC ACID IN n-HEXANE MEDIUM
AbstractThe performance of loofa-immobilized Rhizopus oryzae (as a whole-cell biocatalyst) in the synthesis of methyl oleate was evaluated using oleic acid as a model substrate. The activities of the cell-bound lipase in terms of the esterification and hydrolysis reactions were found to be higher for the immobilized cells as compared with those of the free c
Braz. J. Chem. Eng.. Publicado em: 2015-06