Environmental Mycobacteria
Mostrando 13-24 de 62 artigos, teses e dissertações.
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13. Bacteriological survey of tuberculous lymphadenitis in southeast England, 1981-1989.
STUDY OBJECTIVE--The aim was to detect any changing trends in the nature and incidence of tuberculous lymphadenitis in southeast England and to determine whether there is any evidence for an increase in this disease that could be related to HIV infection. DESIGN--Mycobacteria isolated from patients with lymphadenitis in the years 1981 to 1989 were identified
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14. Optimization of Procedures for Isolation of Mycobacteria from Soil and Water Samples Obtained in Northern India
For isolation of environmental mycobacteria, a decontamination procedure has been standardized by which treatment with 3% sodium dodecyl sulfate plus 4% NaOH (15 and 30 min for rapid and slow growers, respectively) is followed by incubation with 2% cetrimide (5 and 15 min for fast- and slow-growing mycobacteria, respectively); this procedure was found to com
American Society for Microbiology.
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15. Isolation and Identification of Environmental Mycobacteria in the Mycobacterium bovis BCG Trial Area of South India
The isolation profiles of environmental mycobacteria present in soil, water, and dust samples, and sputum samples of persons with symptoms of chest infection in the South Indian Mycobacterium bovis BCG (bacillus Calmette-Guérin) trial area were compared. Isolates belonging to the Mycobacterium avium-intracellulare-scrofulaceum complex were predominant in wa
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16. Interaction of Mycobacterium avium with environmental amoebae enhances virulence.
Environmental mycobacteria are a common cause of human infections. Recently, contaminated domestic water supplies have been suggested as a potential environmental source of several mycobacterial diseases. Since many of these mycobacterial species replicate best intracellularly, environmental hosts have been sought. In the present study, we examined the inter
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17. Cross-Reactive Immunity to Mycobacterium tuberculosis DosR Regulon-Encoded Antigens in Individuals Infected with Environmental, Nontuberculous Mycobacteria▿ †
Mycobacterium tuberculosis DosR regulon-encoded antigens are highly immunogenic in M. tuberculosis-infected humans and are associated with latent tuberculosis infection. We have investigated the hypothesis that infection with or exposure to nontuberculous mycobacteria (NTM) can induce cross-reactive immunity to M. tuberculosis DosR regulon-encoded antigens s
American Society for Microbiology (ASM).
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18. Mycobacterial contamination of fibreoptic bronchoscopes.
Contamination of bronchoalveolar lavage specimens by environmental mycobacteria in hospital water supplies may lead to diagnostic confusion, particularly in immunocompromised patients. Mycobacteria may become concentrated in the tubing of bronchoscope disinfecting machines. It is very difficult to eradicate these organisms once contamination has occurred.
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19. Age-dependent humoral responses of children to mycobacterial antigens.
In the United States, disseminated infection with environmental mycobacteria, including the Mycobacterium avium complex, is the most common opportunistic bacterial infection seen in AIDS patients. However, the source and relative degree of exposure to environmental mycobacteria during childhood are unknown. To examine the age-related exposure to mycobacteria
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20. Agents of newly recognized or infrequently encountered mycobacterial diseases.
This paper reviews recent information on the systematics and clinical significance of potentially pathogenic environmental mycobacteria. A short history of these mycobacteria is given. Information on species for which clinical and systematic aspects have already been well documented, i.e., Mycobacterium kansasii, M. marinum, M. scrofulaceum, M. simiae, M. sz
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21. The Environmental Pathogen Mycobacterium ulcerans Grows in Amphibian Cells at Low Temperatures
Mycobacterium ulcerans, the etiological agent of Buruli ulcers, is an environmental pathogen. We cultivated it in an amphibian (XTC-2) cell line that grows at 28°C. By counting of Ziehl-Neelsen-stained mycobacteria and by quantitative PCR analysis, we found that M. ulcerans multiplies rapidly in association with XTC-2 cells. Transmission electron microscopy
American Society for Microbiology.
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22. Influence of Environmental Gradients on the Abundance and Distribution of Mycobacterium spp. in a Coastal Lagoon Estuary▿
Environmental mycobacteria are of increasing concern in terms of the diseases they cause in both humans and animals. Although they are considered to be ubiquitous in aquatic environments, few studies have examined their ecology, and no ecological studies of coastal marine systems have been conducted. This study uses indirect gradient analysis to illustrate t
American Society for Microbiology (ASM).
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23. Antibodies to mycobacterial peptidoglycolipid and to crude protein antigens in sera from different categories of human subjects.
Sera from patients with disease caused by the Mycobacterium avium complex (M. avium and M. intracellulare), M. kansasii, or M. tuberculosis and from subjects who did not have a mycobacterial disease were tested by enzyme-linked immunosorbent assay against peptidoglycolipid antigens representing each of the 15 most common serovars of the M. avium complex and
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24. Development of a Single-Tube, Cell Lysis-Based, Genus-Specific PCR Method for Rapid Identification of Mycobacteria: Optimization of Cell Lysis, PCR Primers and Conditions, and Restriction Pattern Analysis
A single-tube PCR method was developed for efficient identification of nontuberculous mycobacteria (NTM) and their environmental isolates in about 3 h without conventional DNA isolation. The following three steps were optimized or developed: (i) a simple, 6-min direct cell lysis protocol as a PCR prestep for generation of DNA-template, (ii) an improved Mycob
American Society for Microbiology.