Environmental Labeling
Mostrando 13-24 de 55 artigos, teses e dissertações.
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13. Evidence for Lack of Turnover of Ribulose 1,5-Diphosphate Carboxylase in Barley Leaves
Turnover of ribulose 1,5-diphosphate carboxylase in barley leaves (Hordeum vulgare L.) was followed over time in light and dark. The enzyme was degraded in prolonged darkness and was resynthesized after the plants were returned to light. Labeling with 14C showed that simultaneous synthesis and degradation (turnover) did not occur in light. In contrast, the r
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14. Differential modulation of the expression of axonal proteins by non-neuronal cells of the peripheral and central nervous system.
Axonal behavior during the formation of the neuronal network of the nervous system has been shown to be under environmental control. Hence, as a first step in a project aiming to elucidate the molecular basis of axonal functions, we have identified axonal proteins whose synthesis is subject to environmentally induced changes. Neurons from chicken embryonic d
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15. Effects of Ozone Exposure during Microarray Posthybridization Washes and Scanning
The increasing prevalence of array-based comparative genomic hybridization in the clinical laboratory necessitates the implementation of quality control measures to attain accurate results with a high level of confidence. Environmental ozone is present in all industrialized cities and has been found to be detrimental to array data even at levels considered a
American Society for Investigative Pathology.
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16. An infection-based model of neurodevelopmental damage
Perinatal exposure to infectious agents and toxins is linked to the pathogenesis of neuropsychiatric disorders, but the mechanisms by which environmental triggers interact with developing immune and neural elements to create neurodevelopmental disturbances are poorly understood. We describe a model for investigating disorders of central nervous system develo
The National Academy of Sciences.
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17. Environmental and Developmental Regulation of the Wound-Induced Cell Wall Protein WI12 in the Halophyte Ice Plant1
A wounded gene WI12 was used as a marker to examine the interaction between biotic stress (wounding) and abiotic stress (high salt) in the facultative halophyte ice plant (Mesembryanthemum crystallinum). The deduced WI12 amino acid sequence has 68% similarity to WUN1, a known potato (Solanum tuberosum) wound-induced protein. Wounding, methyl jasmonate, and p
American Society of Plant Physiologists.
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18. Reactivation of the Bacillus subtilis anti-sigma B antagonist, RsbV, by stress- or starvation-induced phosphatase activities.
sigma B is a secondary sigma factor that controls the general stress regulon in Bacillus subtilis. The regulon is activated when sigma B is released from a complex with an anti-sigma B protein (RsbW) and becomes free to associate with RNA polymerase. Two separate mechanisms cause sigma B release: an ATP-responsive mechanism that correlates with nutritional s
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19. A 90-kD Phospholipase D from Tobacco Binds to Microtubules and the Plasma Membrane
The organization of microtubule arrays in the plant cell cortex involves interactions with the plasma membrane, presumably through protein bridges. We have used immunochemistry and monoclonal antibody 6G5 against a candidate bridge protein, a 90-kD tubulin binding protein (p90) from tobacco BY-2 membranes, to characterize the protein and isolate the correspo
American Society of Plant Biologists.
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20. Altered Patterns of Sucrose Synthase Phosphorylation and Localization Precede Callose Induction and Root Tip Death in Anoxic Maize Seedlings12
Root extracts made from maize (Zea mays) seedlings submerged for 2 h showed an increased 32P-labeling of a 90-kD polypeptide in a Ca2+-dependent manner. This protein was identified as sucrose synthase (SS) by immunoprecipitation and mutant analysis. Metabolic labeling with 32Pi indicated that the aerobic levels of SS phosphorylation were maintained up
American Society of Plant Physiologists.
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21. Compartmentation of uracil in Euglena gracilis.
Compartmentation of uracil in the flagellate Euglena gracilis was studied by tracer-kinetic experiments. Lag times in the equilibration of exogenously given and intracellularly present uracil before linear labeling of catabolic and anabolic products was determined to estimate the size of its metabolically active pool. This pool operates in the incorporation
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22. Evidence for a Transient Association of New Proteins with the Spirulina maxima Phycobilisome in Relation to Light Intensity.
Environmental parameters are known to affect phycobilisomes. Variations of their structure and relative composition in phycobiliproteins have been observed. We studied the effect of irradiance variations on the phycobilisome structure in the cyanobacterium Spirulina maxima and discovered the appearance of new polypeptides associated with the phycobilisomes u
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23. Identification of point mutations in mixtures by capillary electrophoresis hybridization.
We have developed a rapid method for unambiguous identification and mutant fraction determination of individual mutants in mixtures of DNA sequence variants each differing by one or a few nucleotides. This method has applications to such diverse areas as interpretation of mutational spectra, screening of populations for polymorphisms and identification of sp
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24. Effect of Heat Shock and Mutations in ClpL and ClpP on Virulence Gene Expression in Streptococcus pneumoniae
Spread of Streptococcus pneumoniae from the nasopharynx to other host tissues would require the organism to adapt to a variety of environmental conditions. Since heat shock proteins are induced by environmental stresses, we investigated the effect of heat shock on ClpL and ClpP synthesis and the effect of clpL and clpP mutations on the expression of key pneu
American Society for Microbiology.