Encephalitozoon Cuniculi
Mostrando 1-12 de 59 artigos, teses e dissertações.
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1. Técnicas de coloração para detecção de Encephalitozoon cuniculi em cortes histológicos
No presente trabalho, foram avaliadas diferentes técnicas de coloração aplicadas a cortes histológicos para a identificação de Encephalitozoon cuniculi. Foram utilizados fragmentos hepáticos de camundongos Balb-c, imunossuprimidos com ciclofosfamida e inoculados com esporos de Encephalitozoon cuniculi. Os cortes histológicos incluídos em parafina fo
Ciência Rural. Publicado em: 26/11/2010
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2. Draft genome sequence of the Daphnia pathogen Octosporea bayeri : insights into the gene content of a large microsporidian genome and a model for host-parasite interactions
Background: The highly compacted 2.9-Mb genome of Encephalitozoon cuniculi placed the microsporidia in the spotlight, encoding a mere 2,000 proteins and a highly reduced suite of biochemical pathways. This extreme level of reduction is not universal across the microsporidia, with genomes known to vary up to sixfold in size, suggesting that some genomes may h
Publicado em: 2010
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3. Meningoencefalomielite experimental por Encephalitozoon cuniculi em camundongos imunossuprimidos com ciclofosfamida
A encefalitozoonose constitui protozoose emergente em indivíduos imunocomprometidos. Este estudo visa examinar o desenvolvimento de infecção experimental por Encephalitozoon cuniculi no sistema nervoso central de camundongos imunossuprimidos. Camundongos Balb-C adultos foram inoculados intraperitonealmente com esporos de E. cuniculi, tratados com ciclofos
Arquivos de Neuro-Psiquiatria. Publicado em: 2005-06
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4. Infecção experimental pelo Encephalitozoon cuniculi em camundongos imunossuprimidos com dexametasona
OBJETIVO: O microsporídio Encephalitozoon cuniculi tem sido reconhecido como um patógeno oportunista em indivíduos imunossuprimidos, tais como pacientes com Aids. O objetivo do trabalho foi desenvolver animais farmacologicamente imunossuprimidos como modelo da infecção natural pelo E. cuniculi. MÉTODOS: Foram usados grupos distintos de camundongos Balb
Revista de Saúde Pública. Publicado em: 2002-10
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5. Enterocytozoon bieneusi (microsporidia) in faecal samples from domestic animals from Galicia, Spain
In this survey we examined 87 domestic animal stool samples in order to detect the possible presence of microsporidia in animals in close contact with humans in Galicia (NW, Spain). The detection of Enterocytozoon bieneusi spores was confirmed in faecal samples from two dogs and one goat by polymerase chain reaction. None of the positive samples for microspo
Memórias do Instituto Oswaldo Cruz. Publicado em: 2002-10
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6. Chitinolytic activity in viable spores of encephalitozoon species
By employing 4-methylumbelliferyl-beta-D-NN',N"-triacetylchitotriose substrate in a semi quantitative assay, chitinolytic activity in viable spores of Encephalitozoon cuniculi and E. intestinalis was detected and dependence on reaction time, spore concentration, concentration of substrate and temperature were demonstrated. It was possible to block the chitin
Memórias do Instituto Oswaldo Cruz. Publicado em: 2000-10
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7. In Vitro Susceptibilities of the Microsporidia Encephalitozoon cuniculi, Encephalitozoon hellem, and Encephalitozoon intestinalis to Albendazole and Its Sulfoxide and Sulfone Metabolites
In vitro comparisons demonstrated that the efficacy of albendazole, albendazole-sulfoxide, and albendazole-sulfone against pathogenic Encephalitozoon species was proportional to the degree of oxidation at a concentration of >10−3 μg/ml. Furthermore, at a concentration of <10−2 μg/ml, benzimidazoles were more effective against Encephalitozoon cuniculi a
American Society for Microbiology.
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8. Monoclonal Antibodies for Specific Detection of Encephalitozoon cuniculi
Seven species-specific monoclonal antibodies (MAbs) were produced against Encephalitozoon cuniculi and characterized. The MAbs were immunoglobulin G, and when used for indirect microimmunofluorescence microscopy and Western immunoblot assays, they detected E. cuniculi originating from clinical samples. They did not cross-react with other Encephalitozoon spec
American Society for Microbiology.
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9. Susceptibility of Encephalitozoon cuniculi to several drugs in vitro.
In the light of the increased incidence of human Encephalitozoon infections and the absence of an established treatment protocol, a simple in vitro testing method to compare activities of drugs against Encephalitozoon cuniculi was developed. With this in vitro method, the 50% inhibitory concentrations of fumagillin, thiabendazole, albendazole, oxibendazole,
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10. A microsporidian isolated from an AIDS patient corresponds to Encephalitozoon cuniculi III, originally isolated from domestic dogs.
The ribosomal DNA internal transcribed spacer (ITS) region of a recently cultured human Encephalitozoon cuniculi isolate was analyzed by gene amplification and DNA sequencing. Restriction endonuclease digestion (FokI) and double-stranded DNA heteroduplex mobility shift analysis were performed to determine their utility for strain differentiation. The human E
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11. Detection of microsporidian spores in clinical samples by indirect fluorescent-antibody assay using whole-cell antisera to Encephalitozoon cuniculi and Encephalitozoon hellem.
Three polyclonal mouse antisera, to Encephalitozoon cuniculi, Nosema algerae, and Nosema corneum, and two polyclonal rabbit antisera, to E. cuniculi and Encephalitozoon hellem, were used in an indirect fluorescent-antibody assay (IFA) with Enterocytozoon bieneusi, E. cuniculi, and Encephalitgozoon. hellem spores (spores of the last two were taken from cultur
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12. In Vitro Effects of Resveratrol on the Viability and Infectivity of the Microsporidian Encephalitozoon cuniculi
Microsporidians of the genus Encephalitozoon are an important cause of disease in immunocompromised patients, and there are currently no completely effective treatments. The present study investigated the viability and infectivity of spores of Encephalitozoon cuniculi that had been exposed to resveratrol (RESV), a natural phytoalexin found in grapes and red
American Society for Microbiology.