Embryo Cultures
Mostrando 13-24 de 421 artigos, teses e dissertações.
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13. Analise do efeito da radiação de microondas sobre Anagasta Kuhniella Zeller (1879) (Lepidoptera: Pyralidae) e seus dois agentes de controle biologico, Bracon hebertor (Say, 1836) (Hymenoptera: Braconidae) e Bacillus thuringiensis var. kurstaki (H:3a3b)
The application of Integrated Pest Management (IPM) means the utilization of efficient control methods which are ecologically safe and economicaly viable. Therefore, it is extremaly important to gather more information about the possible interactions between the different control agents, aimimg to optimize such a method. Microwaves (300 a 300.000 MHz), as we
Publicado em: 1998
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14. Cultura de tecidos de Phaseolus vulgaris L
Tissues of 36 cultivars of Phaseolus vulgaris L. wee established in vitro aiming toward the development of a protocol for plant differentiation from bean tissues in order to apply the in vitro techniques for the improvement of this species. Callus cultures of Phaseolus vulgaris cv. Moruna were established in the presence of MS medium supplemented with KIN an
Publicado em: 1982
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15. Transformation of Rat and Hamster Embryo Cells by Extracts of City Smog
Extracts of particulate matter from condensates of city air were tested for their ability to transform rat or hamster cell cultures. Uninfected rat embryo cultures were not transformed, but cultures chronically infected with Rauscher leukemia virus were transformed by benzpyrene or by extracts of city smog. The smog extracts were 600 times more active than p
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16. Comparative Immunogenicities of Chikungunya Vaccines Propagated in Monkey Kidney Monolayers and Chick Embryo Suspension Cultures
A comparative study was made of Formalin-inactivated Chikungunya vaccines prepared from the virus propagated in African green monkey kidney monolayers and concentrated chick embryo suspension cultures. The vaccine prepared in the chick embryo suspension cultures was significantly more protective to mice against a live homologous virus challenge and stimulate
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17. Production of zebrafish germ-line chimeras from embryo cell cultures
Although the zebrafish possesses many characteristics that make it a valuable model for genetic studies of vertebrate development, one deficiency of this model system is the absence of methods for cell-mediated gene transfer and targeted gene inactivation. In mice, embryonic stem cell cultures are routinely used for gene transfer and provide the advanta
The National Academy of Sciences.
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18. Activation of Nonexpressed Endogenous Ecotropic Murine Leukemia Virus by Transfection of Genomic DNA into Embryo Cells
We studied the infectivity of endogenous ecotropic murine leukemia virus genomes contained in high-molecular-weight DNA prepared from virus-free cells of the AKR-2B line, and from RF, BALB/c, B6, and (BALB/c × B6)F1 mouse embryo cells. When DNA prepared from virus-free AKR-2B cells was transfected into NIH-3T3 cells, no virus-positive cultures were observed
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19. Crystallin gene expression and lentoid body formation in quail embryo neuroretina cultures transformed by the oncogenic retrovirus Mill Hill 2 or Rous sarcoma virus.
The lens-specific proteins alpha and delta crystallins and lentoid bodies, structures that follow a differentiation pathway similar to that of the lens, regularly appear after 4 to 5 weeks in quail embryo neuroretina monolayer cultures. We have investigated the effects of the avian oncogenic retroviruses Mill Hill 2 and Rous sarcoma virus on this process. Qu
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20. Comparison of three rocky mountain spotted fever vaccines.
Growth of Rocky Mountain spotted fever (RMSF) rickettsiae in duck embryo cell (DEC) cultures and chicken embryo cell (CEC) cultures was evaluated. Experimental lots of duck embryo cell- and chicken embryo cell-grown Rocky Mountain spotted fever vaccines and a commercial lot of yolk sac-grown vaccine were compared for protective efficacy in rhesus monkeys. In
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21. Use of guinea pig embryo cell cultures for isolation and propagation of group A coxsackieviruses.
The isolation of group A coxsackieviruses from clinical specimens generally requires the use of suckling mice. By using guinea pig embryo cells, the following coxsackieviruses were isolated from throat swabs and stool samples obtained from patients with a variety of illnesses: two of type A2, one each of types A6 and A8, and four of type 10. Distinct cytopat
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22. Use of a Clonal Line of Porcine Kidney Cell Cultures for Primary Isolation and Vaccine Studies with Adenoviruses
The clonal line (Y15) of porcine kidney stable cells provided a recovery system for adenovirus T4 from specimens from adults with respiratory illnesses that was as sensitive as human embryo kidney cultures. Adenoviruses T7 from adults, and T1, 2, 3, and 5 from children could be readily isolated in porcine kidney cell cultures. The latter were useful for aden
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23. Effect of Interferon on Deoxyribonuclease Induction in Chick Fibroblast Cultures Infected with Cowpox Virus
An exonuclease which degrades native deoxyribonucleic acid at pH 9.2 was induced in chick embryo fibroblast cultures and in human amnion cells by infection with cowpox virus. Highly purified chick embryo interferon suppressed the induction of the enzyme in the homologous cell system but not in the human amnion cell cultures. “Mock” interferon prepared fr
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24. EFFECT OF 6-AMINONICOTINAMIDE ON CHICK-EMBRYO TISSUE CULTURES INFECTED WITH VACCINIA VIRUS
Lee, Spencer H. S. (Dalhousie University, Halifax, Nova Scotia). Effect of 6-aminonicotinamide on chick-embryo tissue cultures infected with vaccinia virus. J. Bacteriol. 88:885–892. 1964.—6-Aminonicotinamide (6-AN) was shown to render chick-embryo fibroblast (CEF) tissue cultures refractory to infection with vaccinia virus. Pretreatment of CEF cultures