Embryo Cryopreserved
Mostrando 1-8 de 8 artigos, teses e dissertações.
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1. Association of L-arginine with heparin on the sperm capacitation improves in vitro embryo production in bovine
Abstract We aimed to evaluate the effects of L-arginine (L-arg) in the quality of in vitro heparin-induced capacitation of cryopreserved bovine spermatozoa and its effects on IVP. The experimental groups were: Control 0 hour without pre-capacitation, and groups of sperm capacitated for 30 min in the absence of COC with heparin (Control 30 min), with 1 mM L-a
Anim. Reprod.. Publicado em: 25/11/2019
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2. Use of conjugated linoleic acid (trans 10, cis 12) to cultivate bovine embryos: effect on cryoresistance and lipid content
ABSTRACT This study evaluated the effect of the addition of conjugated linoleic acid (CLA) to in vitro culture on viability, lipid content, and cryoresistance of bovine embryos at different in vitro culture times. Cumulus oocyte complexes (N = 974) were maturated in vitro for 22 h. In vitro fecundation ensued for 18 h. Viable zygotes were cultivated in vitro
R. Bras. Zootec.. Publicado em: 30/09/2019
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3. Phospholipid composition and resistance to vitrification of in vivo blastocyst of a Brazilian naturalized porcine breed
RESUMO Blastocistos de suínos foram submetidos ao MALDI-TOF para se identificarem os principais fosfolipídios (PL). Depois, parte destes embriões (D6) foram vitrificados (n=52), ou permaneceram frescos (grupo controle, n=42). Após o aquecimento, os blastocistos foram cultivados in vitro para se avaliar a reexpansão e a eclosão (BE) às 24 e 48 horas. F
Arq. Bras. Med. Vet. Zootec.. Publicado em: 14/06/2019
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4. Proteção da vida humana embrionária e repercussão no campo jurídico e ambiental
O avanço da ciência, em especial na área da reprodução humana, permitiu a ocorrência de concepção humana de forma extracorpórea através do processo de fecundação in vitro a ser feita em laboratório especializado em reprodução humana assistida. Os embriões não implantados em útero materno são criopreservados. Este trabalho busca trazer os q
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 29/04/2011
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5. Evaluation of different cryoprotectant and Forskolin in the culture medium for improving the efficacy of vitrification of Bos indicus in vitro derived embryos / Uso de propanediol ou DMSO na vitrificação de embriões produzidos in vitro, cultivados ou não na presença de forskolin
Considering the in vitro method of embryo production, field results have shown a lower resistance to cryopreservation for B. indicus, when compared to B. taurus embryos. A possible explanation for this is a great concentration of lipid droplets in the cytoplasm of cells from B. indicus embryos. The objective of this study was to compare two cryoprotectants (
Publicado em: 2009
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6. Criopreservação oocitária: desempenho reprodutivo na fertilização assistida.
The advance of in vitro fertilization (IVF) caused the emergence of a few dilemmas that forcibly led to deep reflections, standing out among these the questioning about the destination of excess embryos originated from this technology. In view of the controversy observed in several cultures as to ethic, moral, religious, political and scientific issues relat
Publicado em: 2006
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7. Effect of external cryoprotectants on the in vitro viability of frozen spermatozoa of SWISS-ALBINA and BALB/c mice (Mus musculus). / Efeito de crioprotetores externos sobre a viabilidade in vitro de espermatozóides congelados de camundongos (Musmusculus) das linhagens SWISSALBINA e BALB/c.
The genetic progress obtained with trangenesis required the development and consolidation of reproductive techniques such as in vitro embryo production and gamete cryopreservation. With the diversity of new models and genetic lines, it became critical to optimize the use of experimental techniques of cryopreservation of semen in different animal species. The
Publicado em: 2006
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8. Long-term effects of embryo freezing in mice.
Embryo cryopreservation does not induce clear-cut anomalies at detectable rates, but several mechanisms exist for nonlethal damage during the freeze-thaw process, and the risk of moderate or delayed consequences has not been extensively investigated. In a long-term study including senescence, we compared cryopreserved and control mice for several quantitativ