Eco Formation
Mostrando 25-36 de 126 artigos, teses e dissertações.
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25. João Batista Vilanova Artigas, arquiteto (1934 1941): a gênese de uma obra / João Batista Vilanova Artigas, arquiteto (1934 1941): a gênese de uma obra
Na História da Arquitetura Brasileira, o arquiteto João Batista Vilanova Artigas (1915-1985) foi o criador da escola paulista inserida no Movimento Modernista brasileiro. Nascido em meio à segunda década do século XX, toda a sua formação básica, intelectual e profissional se fez entre um tempo final da Primeira República e uma parte da República No
Publicado em: 2006
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26. Formacao universitaria de professores para o ensino da linguagem escrita: um estudo a partir dos discursos didatico-formadores
The study aimed at analyzing the theoretical-methodological treatment given to the teaching of Written Language in a process of education teachers-literacy teachers , taking into account the nature of that professional role in the triad didactic: teacher/ student/ object- writing. The initial idea was that the knowledge of writing is enrolled in the story of
Publicado em: 2006
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27. Modificação de poliamida 6 com elastomeros de epicloridrina
Blends of polyamide 6 (PA 6) and epichlorohydrin elastomers, poliepichlorohydrin (PEPI) and poli(epichlorohydrin-co-ethylene oxide), ECO, with dífferent compositions were prepared by mechanical mixture using a Banbury type mixer. The characterization of the resulting materiaIs was made by Differential Scanning Calorimetry (DSC), Dynamic Mechanical Analysis
Publicado em: 1997
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28. Inducible expression and cytogenetic effects of the EcoRI restriction endonuclease in Chinese hamster ovary cells.
The cytogenetic endpoints sister chromatid exchange (SCE) and chromosome aberrations are widely used as indicators of DNA damage induced by mutagenic carcinogens. Chromosome aberrations appear to result directly from DNA double-strand breaks, but the lesion(s) giving rise to SCE formation remains unknown. Most compounds that induce SCEs induce a spectrum of
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29. Site-specific inhibition of EcoRI restriction/modification enzymes by a DNA triple helix.
The ability of oligopyrimidines to inhibit, through triple helix formation, the specific protein-DNA interactions of the EcoRI restriction and modification enzymes (EcoRI and MEcoRI) with their recognition sequence (GAATTC) was studied. The oligonucleotides (CTT)4 and (CTT)8 formed triplexes in plasmids at (GAA)n repeats containing EcoRI sites. Cleavage and
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30. Molecular cloning of DNA from F sex factor of Escherichia coli K-12.
We describe the molecular cloning of various DNA segments generated by partial EcoRI endonuclease digestion of the sex factor F. These segments have been analyzed by agarose gel electrophoresis of EcoRI digests and were arranged in a series of overlapping fragments using the EcoRI fragment map of F established by H. Ohtsubo and E. Ohtsubo. The clones isolate
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31. On the Deletion of Inverted Repeated DNA in Escherichia Coli: Effects of Length, Thermal Stability, and Cruciform Formation in Vivo
We have studied the deletion of inverted repeats cloned into the EcoRI site within the CAT gene of plasmid pBR325. A cloned inverted repeat constitutes a palindrome that includes both EcoRI sites flanking the insert. In addition, the two EcoRI sites represent direct repeats flanking a region of palindromic symmetry. A current model for deletion between direc
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32. Transcription of the Genome of Bacteriophage φ29: Isolation and Mapping of the Major Early mRNA Synthesized In Vivo and In Vitro
The φ29 early mRNA's synthesized in infected Bacillus subtilis were studied by using sedimentation velocity analysis, polyacrylamide gel electrophoresis, and hybridization of φ29 DNA fragments generated by the restriction endonuclease Eco RI. Viral RNAs synthesized in vivo in the resence of chloramphenicol were found to hybridize to Eco RI-A, -C, and -D fr
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33. Cleavage of Replicating Forms of Mitochondrial DNA by EcoRI Endonuclease
Digestion of mouse L cell mitochondrial DNA with EcoRI restriction endonuclease produces two linear duplex fragments comprising 86.3 ± 2.0% and 14.2 ± 1.0% of the circular genome length (16,000 ± 470 nucleotide pairs). Digestion of human HeLa cell mitochondrial DNA with EcoRI produces three linear duplex fragments comprising 49.2 ± 1.0%, 44.4 ± 0.9%, an
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34. Interaction of EcoRII restriction and modification enzymes with synthetic DNA fragments. VI. The binding and cleavage of substrates containing nucleotide analogs.
The present study deals with the binding and cleavage by EcoRII endonuclease of concatemer DNA duplexes containing EcoRII recognition sites (formula; see text) in which dT is replaced by dU or 5-bromodeoxyuridine, or 5'-terminal dC in the dT-containing strand is methylated at position 5. The enzyme molecule is found to interact with the methyl group of the d
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35. Involvement of outside DNA sequences in the major kinetic path by which EcoRI endonuclease locates and leaves its recognition sequence.
We have examined the kinetics of the interaction between endodeoxyribonuclease EcoRI (EC 3.1.23.13) and nine linear DNA fragments that range in size between 34 and 6,200 base pairs and contain the EcoRI site of plasmid pBR322 in a central location. The kinetic parameters governing both formation and decay of specific endonuclease . DNA complexes increase 8-f
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36. Changes in solvation during DNA binding and cleavage are critical to altered specificity of the EcoRI endonuclease
Restriction endonucleases such as EcoRI bind and cleave DNA with great specificity and represent a paradigm for protein–DNA interactions and molecular recognition. Using osmotic pressure to induce water release, we demonstrate the participation of bound waters in the sequence discrimination of substrate DNA by EcoRI. Changes in solvation can play a critica
The National Academy of Sciences.