Dynamic Instability Frequency
Mostrando 13-24 de 25 artigos, teses e dissertações.
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13. Alteration of Microtubule Dynamic Instability during Preprophase Band Formation Revealed by Yellow Fluorescent Protein–CLIP170 Microtubule Plus-End LabelingW⃞
At the onset of mitosis, plant cells form a microtubular preprophase band that defines the plane of cell division, but the mechanism of its formation remains a mystery. Here, we describe the use of mammalian yellow fluorescent protein–tagged CLIP170 to visualize the dynamic plus ends of plant microtubules in transfected cowpea protoplasts and in stably tra
American Society of Plant Biologists.
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14. Nanomolar concentrations of nocodazole alter microtubule dynamic instability in vivo and in vitro.
Previous studies demonstrated that nanomolar concentrations of nocodazole can block cells in mitosis without net microtubule disassembly and resulted in the hypothesis that this block was due to a nocodazole-induced stabilization of microtubules. We tested this hypothesis by examining the effects of nanomolar concentrations of nocodazole on microtubule dynam
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15. Regulation of Microtubule Dynamic Instability in Vitro by Differentially Phosphorylated Stathmin*
Stathmin is an important regulator of microtubule polymerization and dynamics. When unphosphorylated it destabilizes microtubules in two ways, by reducing the microtubule polymer mass through sequestration of soluble tubulin into an assembly-incompetent T2S complex (two α:β tubulin dimers per molecule of stathmin), and by increasing the switching frequency
American Society for Biochemistry and Molecular Biology.
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16. Microtubule Dynamics in Living Root Hairs: Transient Slowing by Lipochitin Oligosaccharide Nodulation SignalsW⃞
The incorporation of a fusion of green fluorescent protein and tubulin-α 6 from Arabidopsis thaliana in root hairs of Lotus japonicus has allowed us to visualize and quantify the dynamic parameters of the cortical microtubules in living root hairs. Analysis of individual microtubule turnover in real time showed that only plus polymer ends contributed to ove
American Society of Plant Biologists.
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17. Vinblastine suppresses dynamics of individual microtubules in living interphase cells.
We have characterized the effects of vinblastine on the dynamic instability behavior of individual microtubules in living BS-C-1 cells microinjected with rhodamine-labeled tubulin and have found that at low concentrations (3-64 nM), vinblastine potently suppresses dynamic instability without causing net microtubule depolymerization. Vinblastine suppressed th
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18. Cell Cycle-Dependent Changes in Microtubule Dynamics in Living Cells Expressing Green Fluorescent Protein-α TubulinV⃞
LLCPK-1 cells were transfected with a green fluorescent protein (GFP)-α tubulin construct and a cell line permanently expressing GFP-α tubulin was established (LLCPK-1α). The mitotic index and doubling time for LLCPK-1α were not significantly different from parental cells. Quantitative immunoblotting showed that 17% of the tubulin in LLCPK-1α cells
The American Society for Cell Biology.
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19. Genetic instability in human ovarian cancer cell lines.
We have analyzed the stability of microsatellites in cell lines derived from human ovarian cancers and found that 5 out of 10 of the ovarian tumor cell lines are genetically unstable at the majority of the loci analyzed. In clones and subclones derived serially from one of these cell lines (2774; serous cystadenocarcinoma), a very high proportion of microsat
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20. The Microtubule-destabilizing Kinesin XKCM1 Regulates Microtubule Dynamic Instability in Cells
The dynamic activities of cellular microtubules (MTs) are tightly regulated by a balance between MT-stabilizing and -destabilizing proteins. Studies in Xenopus egg extracts have shown that the major MT destabilizer during interphase and mitosis is the kinesin-related protein XKCM1, which depolymerizes MT ends in an ATP-dependent manner. Herein, we examine th
The American Society for Cell Biology.
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21. Instability in human forearm movements studied with feed-back-controlled muscle vibration.
1. Frequency-modulated vibration was applied to the elbow flexor and extensor tendons to produce reflex movements of the forearm in normal subjects. The modulating (command) signal caused equal and opposite deviations from the 40 Hz carrier frequency so that when flexor vibratory frequency increased, extensor frequency decreased, and vice versa. 2. It is arg
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22. GTP Is Required for the Microtubule Catastrophe-Inducing Activity of MAP200, a Tobacco Homolog of XMAP2151[W]
Widely conserved among eukaryotes, the microtubule-associated protein 215 (MAP215) family enhances microtubule dynamic instability. The family member studied most extensively, Xenopus laevis XMAP215, has been reported to enhance both assembly and disassembly parameters, although the mechanism whereby one protein can exert these apparently contradictory effec
American Society of Plant Biologists.
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23. Physical basis of two-tone interference in hearing
The cochlea uses active amplification to capture faint sounds. It has been proposed that the amplifier comprises a set of self-tuned critical oscillators: each hair cell contains a force-generating dynamical system that is maintained at the threshold of an oscillatory instability, or Hopf bifurcation. While the active response to a pure tone provides frequen
The National Academy of Sciences.
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24. Chromosome instability associated with human alphoid DNA transfected into the Chinese hamster genome.
Repetitive DNA sequences have been implicated in the mediation of DNA rearrangement in mammalian cells. We have tested this hypothesis by using a dihydrofolate reductase (DHFR) expression vector into which candidate sequences were inserted. DHFR- Chinese hamster ovary (CHO) cells were transfected with this vector, the amplification of which was then selected