Dna Topoisomerase Type Ii
Mostrando 1-12 de 146 artigos, teses e dissertações.
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1. Cinética celular na endometriose profunda infiltrativa de reto-sigmoide: estudo anátomo-clínico / Cell kinetics in deep infiltrating endometriosis of rectosigmoid: an anatomoclinical study
INTRODUÇÃO: A endometriose, uma doença benigna, tem características invasivas com potencial proliferativo. O desenvolvimento das lesões pode ocorrer em decorrência de crescimento celular glandular e/ou estromal ou de alterações na cinética celular. Cinética celular refere-se ao equilíbrio entre a morte celular, ou apoptose, e a proliferação celu
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 13/09/2011
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2. Caracterização genômica da bactéria endossimbiótica do tripanosomatídeo Crithidia deanei e de suas DNA topoisomerases / Genomics characterization of the endosymbiont bacterium of the tripanosomatídeo Crithidia deanei and of its DNA topoisomerases
The present work is focused in the endosymbiont type II topoisomerases, due to their essential roles not only in the replication and transcription processes but also at the recombination and chromosome segregation processes. There are two Type II topoisomerases in bacteria: DNA gyrase and DNA Topoisomerase IV. DNA gyrase is the only topoisomerase capable of
Publicado em: 2006
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3. DNA topoisomerase inhibitors: biflavonoids from Ouratea species
Topoisomerase inhibitors are agents with anticancer activity. 7"-O-Methyl-agathisflavone (I) and amentoflavone (II) are biflavonoids and were isolated from the Brazilian plants Ouratea hexasperma and O. semiserrata, respectively. These biflavonoids and the acetyl derivative of II (IIa) are inhibitors of human DNA topoisomerases I at 200 µM, as demonstrated
Brazilian Journal of Medical and Biological Research. Publicado em: 2002-07
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4. Topoisomerase II cleavage of herpes simplex virus type 1 DNA in vivo is replication dependent.
The genome of herpes simplex virus type 1 contains a large number of recognition sites for eucaryotic DNA type II topoisomerase. Topoisomerase II sites were identified by means of the consensus sequence described previously (J.R. Spitzner and M.T. Muller, Nucleic Acids Res. 16:5553-5556, 1988) and then confirmed by sequencing DNA cleavages introduced by puri
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5. Effects of VM26, a specific inhibitor of type II DNA topoisomerase, on SV40 chromatin replication in vitro.
We have examined the influence of VM26 (teniposide), a specific inhibitor of mammalian type II DNA topoisomerase, on the replication of SV40 minichromosomes in vitro. The replication system we used consists of replicative intermediate SV40 chromatin as substrate which is converted to mature SV40 chromatin in the presence of ATP, deoxynucleotides and a protei
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6. Association between the p170 form of human topoisomerase II and progeny viral DNA in cells infected with herpes simplex virus type 1.
Endogenous host topoisomerase II acts upon herpes simplex virus type 1 (HSV-1) DNA in infected cells (S.N. Ebert, S.S. Shtrom, and M.T. Muller, J. Virol. 56:4059-4066, 1990), and cleavage is directed exclusively at progeny viral DNA while parental DNA is resistant. To evaluate the possibility that HSV-1 induces topoisomerase II activity which could account f
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7. Importance of the Fourth Alpha-Helix within the CAP Homology Domain of Type II Topoisomerase for DNA Cleavage Site Recognition and Quinolone Action
We report that point mutations causing alteration of the fourth alpha-helix (α4-helix) of the CAP homology domain of eukaryotic (Saccharomyces cerevisiae) type II topoisomerases (Ser740Trp, Gln743Pro, and Thr744Pro) change the selection of type II topoisomerase-mediated DNA cleavage sites promoted by Ca2+ or produced by etoposide, the fluoroquinolone CP-115
American Society for Microbiology.
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8. DNA topoisomerase II activity in nonreplicating, transcriptionally inactive, chicken late spermatids.
To study a possible differential involvement of type I and type II DNA topoisomerases in the functional and structural changes that chromatin undergoes during spermatogenesis, we have determined both enzymatic activities in chicken testis cell nuclei at successive stages of differentiation. Whereas DNA topoisomerase I varies in parallel with transcriptional
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9. Inhibition of calf thymus type II DNA topoisomerase by poly(ADP-ribosylation).
The effect of poly(ADP-ribosylation) on calf thymus topoisomerase type II reactions has been investigated. Unknotting of phage P4 head DNA, and relaxation and catenation of supercoiled PM2 DNA are inhibited. We conclude that the inhibition results from poly(ADP-ribosylation) on the following grounds. Firstly, the enzyme poly(ADP-ribose) (PADPR) synthetase an
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10. Inhibitory Activities of Gatifloxacin (AM-1155), a Newly Developed Fluoroquinolone, against Bacterial and Mammalian Type II Topoisomerases
We determined the inhibitory activities of gatifloxacin against Staphylococcus aureus topoisomerase IV, Escherichia coli DNA gyrase, and HeLa cell topoisomerase II and compared them with those of several quinolones. The inhibitory activities of quinolones against these type II topoisomerases significantly correlated with their antibacterial activities or cyt
American Society for Microbiology.
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11. Yeast recombination pathways triggered by topoisomerase II-mediated DNA breaks
Topoisomerase II is a ubiquitous enzyme that removes knots and tangles from the genetic material by generating transient double-strand DNA breaks. While the enzyme cannot perform its essential cellular functions without cleaving DNA, this scission activity is inherently dangerous to chromosomal integrity. In fact, etoposide and other clinically important ant
Oxford University Press.
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12. Antibacterial Activities and Inhibitory Effects of Sitafloxacin (DU-6859a) and Its Optical Isomers against Type II Topoisomerases
The in vitro inhibitory effects of sitafloxacin (DU-6859a) and its three stereoisomers on bacterial DNA gyrase from Escherichia coli, topoisomerase IV from Staphylococcus aureus, and topoisomerase II from human placenta were compared. No correlation was observed between the inhibitory activities of quinolones against bacterial type II topoisomerases and thos
American Society for Microbiology.