Dna Solution
Mostrando 13-24 de 1170 artigos, teses e dissertações.
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13. Polymerase chain reaction (PCR) identification of terverticillate Penicillium species isolated from agricultural soils in eskişehir province
In the present study, nine terverticillate Penicillium isolates (P. griseofulfum, P. puberulum, P. crustosum, P. aurantiogriseum, P. chrysogenum, P. primulinum, P. expansum, P. viridicatum, Eupenicillium egyptiacum) from 56 soil samples were characterized genetically by a PCR method. The DNAs of the strains were isolated using the glass beads and vortexing e
Braz. arch. biol. technol.. Publicado em: 25/10/2013
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14. An easy and efficient strategy for KEL genotyping in a multiethnic population
BACKGROUND: The Kell blood group system expresses high and low frequency antigens with the most important in relation to transfusion including the antithetic KEL1 and KEL2; KEL3 and KEL4; KEL6 and KEL7 antigens. Kell is a clinically relevant system, as it is highly immunogenic and anti-KEL antibodies are associated with hemolytic transfusion reactions and he
Rev. Bras. Hematol. Hemoter.. Publicado em: 2013
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15. Estudo dos produtos químicos originados a partir da degradação do gel de clorexidina a 2%, por meio da Cromatografia Gasosa acoplada à Espectrometria de Massas / Study of chemicals derived from the degradation of 2 % chlorhexidine gel, by means gas chromatography coupled to mass spectrometry
The aim of this study was to determine, by means of gas chromatography coupled to mass spectrometry (GC-MS), products originated from the degradation of 2 % chlorhexidine digluconate (CHX) gel. Three gels were selected: commercial Gel_Chlorhexidina® (Maquira), 2 % CHX gel UNIARARAS (handled at the Pharmacy Education UNIARARAS) and 2% CHX gel prepared in lab
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 23/01/2012
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16. Buccal cells DNA extraction to obtain high quality human genomic DNA suitable for polymorphism genotyping by PCR-RFLP and Real-Time PCR
OBJECTIVE: The aim of this study was to evaluate, by PCR-RFLP and real-time PCR, the yield and quality of genomic DNA collected from buccal cells by mouthwash after different storage times at room temperature. MATERIAL AND METHODS: A group of volunteers was recruited to collect buccal cells using a mouthwash solution. The collected solution was divided into
J. Appl. Oral Sci.. Publicado em: 2012-08
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17. Integrated experimental biophysics and molecular dynamics simulations of biomolecules in solution - the interaction of nuclear receptors with DNA response elements and the inter-domain dynamics of Cellobiohydrolase I / Estudos por modelagem e dinâmica molecular integradas a técnicas físicas para biomoléculas em solução - interação de receptores nucleares a elementos responsivos no DNA e dinâmica inter-domínios da celobiohidrolase I
Collective motions play a fundamental role in solution biomolecule dynamics and energetics. These movements can couple very distant regions in the protein structures affection, for instance, allosteric mechanisms, the establishment of macromolecular complexes, and on the integrated function of multidomain proteins as molecullar machines. In this thesis, we p
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 26/09/2011
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18. Estudos funcionais da proteína reguladora humana Ki-1/57 e seus parceiros de interação / Functional studies of the human regulatory protein Ki-1/57 and its interaction partners
The protein Ki-1/57 was discovered through cross reactivity of the monoclonal antibody Ki-1 in cells of Hodgkin lymphoma. Previously studies demonstrated that Ki-1/57 interacts with RACK-1 protein, undergoes phosphorylation by PKCs and methylation by PRMT1, an arginine methyltransferase that modulates several RNA binding proteins. Studies have shown that Ki-
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 12/07/2011
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19. Estudos estruturais e funcionais das proteínas cinases humanas Nek1 e Nek6 / Structural and functional studies of Nek1 Nek6 protein kinases
NIMA was identified and functionally characterized in Aspergillus nidulans as a critical Ser/Thr kinase for cell cycle progression. The mammalian Neks (NIMA-related kinases) represent an evolutionarily conserved family of 11 serine/threonine kinases that share 40-45% identity with NIMA N-terminal domain. Neks are associated to cell cyclerelated functions and
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 04/03/2011
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20. Studying nanotoxic effects of CdTe quantum dots in Trypanosoma cruzi
Semiconductor nanoparticles, such as quantum dots (QDs), were used to carry out experiments in vivo and ex vivo with Trypanosoma cruzi. However, questions have been raised regarding the nanotoxicity of QDs in living cells, microorganisms, tissues and whole animals. The objective of this paper was to conduct a QD nanotoxicity study on living T. cruzi protozoa
Memórias do Instituto Oswaldo Cruz. Publicado em: 2011-03
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21. Assessment of the genetic risks of a metallic alloy used in medical implants
The use of artificial implants provides a palliative or permanent solution for individuals who have lost some bodily function through disease, an accident or natural wear. This functional loss can be compensated for by the use of medical devices produced from special biomaterials. Titanium alloy (Ti-6Al-4V) is a well-established primary metallic biomaterial
Genetics and Molecular Biology. Publicado em: 07/01/2011
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22. Aluminum-induced genes in grass species.
This study aimed to identify genes associated with Al tolerance mechanisms in grasses, using cDNA and subtracitve libraries derived from roots of maize, rice, sorghum, oat, barley, wheat and brachiaria tolerant genotypes submitted to critical levels of Al in nutrient solution.
INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY. Publicado em: 2011
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23. Analise de "RFLP" da tolerancia a toxidez do aluminio no cromossomo 2 do milho.
O presente trabalho objetivou, via tecnica de RFLP, localizar, no cromossomo 2 do milho, regioes genomicas relacionadas com a resistencia ao aluminio toxico, o qual encontra-se presente em cerca de 60% do territorio brasileiro. Linhagens de milho tolerantes (15, 16, 699 e 1327) e suscetiveis (19, 53 e 57) ao aluminio, foram utilizadas nos cruzamentos 57 x 1.
Pesquisa Agropecuaria Brasileira. Publicado em: 2011
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24. HISTOLOGIA, FUNÇÃO COCLEAR E GENOTOXICIDADE EM COBAIAS TRATADAS COM CISPLATINA / HISTOLOGY, COCHLEAR FUNCTION AND GENOTOXICITY IN GUINEA PIG TREATED WITH CISPLATIN
The present work aims to investigate the cisplatin influence on the cochlea and the deoxiribonucleic acid (DNA) of guinea pigs. An experimental study was executed with 12 guinea pigs (Cavia porcellus). The inclusion criterion for guinea pigs in the sample was the presence of Preyer s reflex (contraction of the pinna when facing sound stimulus) and distortion
Publicado em: 2011