Dna Quantification
Mostrando 13-24 de 405 artigos, teses e dissertações.
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13. Successful and failed mini-implants: microbiological evaluation and quantification of bacterial endotoxin
Abstract Objectives Using two groups of mini-implants (successful and failed) the objectives of this in vivo study were: to evaluate the microbial contamination by the checkerboard DNA-DNA hybridization technique and to quantify the bacterial endotoxin by the limulus amebocyte lysate assay. Material and Methods The 15 successful and 10 failed mini-implan
J. Appl. Oral Sci.. Publicado em: 10/07/2018
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14. Polyphenols From Cutch Tree ( Acacia catechu Willd.): Normalize In Vitro Oxidative Stress and Exerts Antiproliferative Activity
ABSTRACT Oxidative stress, being the main cause of most of the human diseases, has always been the highlight of research worldwide. This stress can be overcome by administration of natural polyphenols. The Acacia catechu Willd. has many refrences available in Ayurveda as important disease curative plant. Its leaves are investigated for ameliorating oxidative
Braz. arch. biol. technol.. Publicado em: 25/06/2018
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15. Circulating cell-free DNA as a biomarker in the diagnosis and prognosis of colorectal cancer
Abstract Colorectal cancer (CRC) is a disease without evident clinical symptoms in early stages, leading to late diagnosis and disease management. Current diagnostic and prognostic tools require invasive procedures and circulating molecular biomarkers fail to have optimal sensitivity and specificity. Circulating biomarkers with high clinical performance may
Braz. J. Pharm. Sci.. Publicado em: 07/06/2018
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16. Unconventional vegetables collected in Brazil: chromosome number and description of nuclear DNA content
Abstract The aim of this study was to perform chromosome counts and nuclear DNA quantification of the unconventional vegetables species: bertalha (Basella alba L.), vinagreira verde (Hibiscus sabdariffa L.), azedinha (Rumex acetosa L.), peixinho (Stachys byzantina K. Koch), and capuchinha (Tropaeolum majus L.). Metaphase chromosomes were obtained from the pr
Crop Breed. Appl. Biotechnol.. Publicado em: 2017-12
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17. Topical application of bFGF on acid-conditioned and non-conditioned dentin: effect on cell proliferation and gene expression in cells relevant for periodontal regeneration
Abstract Periodontal regeneration is still a challenge in terms of predictability and magnitude of effect. In this study we assess the biological effects of combining chemical root conditioning and biological mediators on three relevant cell types for periodontal regeneration. Material and Methods: Bovine dentin slices were conditioned with 25% citric acid
J. Appl. Oral Sci.. Publicado em: 2017-12
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18. Decellularization of placentas: establishing a protocol
Biological biomaterials for tissue engineering purposes can be produced through tissue and/or organ decellularization. The remaining extracellular matrix (ECM) must be acellular and preserve its proteins and physical features. Placentas are organs of great interest because they are discarded after birth and present large amounts of ECM. Protocols for decellu
Braz J Med Biol Res. Publicado em: 17/11/2017
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19. Polyphenols From Cutch Tree (Acacia catechu Willd.): Normalize In Vitro Oxidative Stress and Exerts Antiproliferative Activity
ABSTRACT Oxidative stress, being the main cause of most of the human diseases, has always been the highlight of research worldwide. This stress can be overcome by administration of natural polyphenols. The Acacia catechu Willd. has many refrences available in Ayurveda as important disease curative plant. Its leaves are investigated for ameliorating oxidative
Braz. arch. biol. technol.. Publicado em: 05/10/2017
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20. Ultrastructural analysis and residual DNA evaluation of rabbit vein scaffold
Abstract Purpose: To investigate the ultrastructural characteristics and analysis of residual DNA in scaffold models, produced with decellularized vena cava in an experimental model with rabbits. Methods: Three groups were created for ultrastructural and residual DNA analysis: group 1 - control, consisting of samples of vena cava in natura; group 2 - SD,
Acta Cir. Bras.. Publicado em: 2017-09
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21. Improved NADH Electroanalysis on Nickel(II) Phthalocyanine Tetrasulfonic Acid/Calf Thymus Deoxyribonucleic Acid/Reduced Graphene Oxide Composite
This work describes the electrochemical performance of a novel composite based on nickel tetrasulfonated phthalocyanine (NiTsPc), deoxyribonucleic acid from calf thymus (CT-DNA) and reduced graphene oxide (rGO) for the electroanalysis of reduced β-nicotinamide adenine dinucleotide (NADH), through electrocatalytic oxidation. The modified electrode was denote
J. Braz. Chem. Soc.. Publicado em: 2017-09
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22. Euphorbia umbellata bark extracts – an in vitro cytotoxic study
ABSTRACT Euphorbia umbellata (Pax) Bruyns, Euphorbiaceae, is commonly used in folk medicine of southern Brazil to treat several kinds of cancer. The latex (part of the plant used for this purpose) is mixed with water and taken as treatment; but this matrix contains toxic potential related to the presence of some phorbol type diterpenes. So the aim of this st
Rev. bras. farmacogn.. Publicado em: 2017-04
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23. Genome-wide analysis of EgEVE_1, a transcriptionally active endogenous viral element associated to small RNAs in Eucalyptus genomes
Abstract Endogenous viral elements (EVEs) are the result of heritable horizontal gene transfer from viruses to hosts. In the last years, several EVE integration events were reported in plants by the exponential availability of sequenced genomes. Eucalyptus grandis is a forest tree species with a sequenced genome that is poorly studied in terms of evolution a
Genet. Mol. Biol.. Publicado em: 23/02/2017
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24. Diagnosis of human herpes virus 1 and 2 (HHV-1 and HHV-2): use of a synthetic standard curve for absolute quantification by real time polymerase chain reaction
The use of quantitative real time polymerase chain reaction (qPCR) for herpesvirus detection has improved the sensitivity and specificity of diagnosis, as it is able to detect shedding episodes in the absence of clinical lesions and diagnose clinical specimens that have low viral loads. With an aim to improve the detection and quantification of herpesvirus b
Mem. Inst. Oswaldo Cruz. Publicado em: 16/02/2017