Dna Quantification
Mostrando 1-12 de 405 artigos, teses e dissertações.
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1. Quantification of cell-free circulating mitochondrial DNA copy number variation in hepatocellular carcinoma
SUMMARY OBJECTIVE: Hepatocellular carcinoma is the most common primary malignant liver tumor. Mitochondrial DNA copy number has been shown to be associated with various malignancies. However, there has not been any study on the absolute quantification of mtDNA copy number in hepatocellular carcinoma. The aim of this study was to develop a new method for abs
Revista da Associação Médica Brasileira. Publicado em: 2022
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2. Quantification of mitochondrial DNA damage and copy number in circulating blood of patients with systemic sclerosis by a qPCR-based assay,
Abstract Background: Although not fully understood, oxidative stress has been implicated in the pathogenesis of different autoimmune diseases such as systemic sclerosis. Accumulating evidence indicates that oxidative stress can induce mitochondrial DNA (mtDNA) damage and variations in mtDNA copy number (mtDNAcn). Objective: The aim of this study was to exp
An. Bras. Dermatol.. Publicado em: 2020-06
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3. Bubaline Diaphragm Matrix: Development and Clinical Assessment into Cattle Abdominal Hernia Repair
Abstract The purpose of the study was to develop a xenogenic bubaline diaphragm matrix (BDM) for abdominal hernia repair. A fresh diaphragm was decellularized using aqueous sodium dodecyl sulfate (SDS) solutions (0.5-4% w/v) over a period. Acellularity was confirmed histologically and characterized by Masson’s trichrome staining, scanning electron microsc
Braz. arch. biol. technol.. Publicado em: 25/11/2019
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4. Validation of reference genes for gene expression studies in bovine oocytes and cumulus cells derived from in vitro maturation
Abstract Quantitative real-time PCR (qPCR) is a valuable tool for gene expression studies and it is necessary to choose an ideal endogenous reference gene for data normalization. This work studied a set of reference genes in oocytes and cumulus cells of COCs (Cumulus-Oocyte Complexes) that are suitable for relative gene expression analyses after in vitro mat
Anim. Reprod.. Publicado em: 25/11/2019
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5. Protective effects of bark ethanolic extract from Spondias dulcis Forst F. against DNA damage induced by benzo[a]pyrene and cyclophosphamide
Abstract This study evaluated the genotoxicity, mutagenicity, antigenotoxicity, and antimutagenicity effects on biochemical parameters of oxidative stress of the Spondias dulcis bark ethanolic extract on mice. The extract was evaluated in the doses of 500, 1000, and 1500 mg/kg bw via gavage. To evaluate the protective effects of the extract, benzo[a]pyrene (
Genet. Mol. Biol.. Publicado em: 14/11/2019
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6. DNA Damage by Endogenous and Exogenous Aldehydes
The present overview describes the formation of deoxyribonucleic acid (DNA) adducts from endogenous and exogenous aldehydes, such as acetaldehyde, acrolein, crotonaldehyde, malonaldehyde, 4-hydroxy-2-nonenal and 2,4-decadienal. Malonaldehyde reacts with 2’-deoxyguanosine, 2’-deoxyadenosine, and 2’-deoxycytidine, yielding cyclic pyrimidopurinone and acy
J. Braz. Chem. Soc.. Publicado em: 21/10/2019
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7. Microbial Profile and Endotoxin Levels in Primary Periodontal Lesions with Secondary Endodontic Involvement
Resumo Este estudo foi realizado para investigar o perfil microbiano e os níveis de endotoxina de lesões endoperiodontais de origem periodontal. Amostras periodontais e endodônticas foram obtidas de bolsas periodontais e canais radiculares necróticos de 10 dentes com lesões endoperiodontais. A investigação de 40 espécies bacterianas diferentes foram
Braz. Dent. J.. Publicado em: 22/07/2019
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8. Temporal variation of bacterial population and response to physical and chemical parameters along a petrochemical industry wastewater treatment plant
Abstract: The petrochemical industry has played a considerable role in generation and release of waste in the environment. Activated sludge and facultative lagoons are commonly used for domestic and industrial wastewater treatment due to their low-cost and minimal need for operational requirements. Microorganisms present in wastewater treatment plant (WWTP)
An. Acad. Bras. Ciênc.. Publicado em: 01/07/2019
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9. Characterization of Decellularized Human Pericardium for Tissue Engineering and Regenerative Medicine Applications
Resumo Fundameto: O enxerto de pericárdio pode ser usado em muitos procedimentos de correção cirúrgica. Uma das estratégias da engenharia tecidual é o processo de descelularização. No entanto, embora esse processo diminua a resposta imunogênica, a descelularização pode modificar tanto o comportamento como a composição da matriz extracelular nat
Arq. Bras. Cardiol.. Publicado em: 27/06/2019
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10. Feline herpesvirus 1 viral load related to environmental factors in sheltered cats
RESUMO: No ambiente do abrigo encontram-se fatores que geram estresse nos animais que ali residem. Esses fatores acabam por provocar a reativação do FHV-1. No entanto, comparações entre carga viral conjuntival e fatores ambientais não foram ainda avaliadas. Objetivo deste estudo foi correlacionar a carga viral de FHV-1 em felinos domésticos com e sem
Cienc. Rural. Publicado em: 13/06/2019
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11. Duplex Real-Time PCR Using Sybr Green I for Quantification and Differential Diagnosis between Salmonella Enteritidis and Salmonella Typhimurium
ABSTRACT The incidence of foodborne diseases caused by the genus Salmonella spp. in industrialized countries is often high in epidemiological surveys. Obtaining a rapid diagnostic test for identification of bacteria is crucial in order to rapidly implement control measures to contain bacterial spread, to reduce losses in animal production and to avoid risks
Braz. J. Poult. Sci.. Publicado em: 09/05/2019
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12. NFAT5 is differentially expressed in Sprague-Dawley rat tissues in response to high salt and high fructose diets
Abstract Current diets contain an increasing amount of salt and high fructose corn syrup, but it remains unclear as to how dietary salt and fructose affect organ function at the molecular level. This study aimed to test the hypothesis that consumption of high salt and fructose diets would increase tissue-specific expression of two critical osmotically-regula
Genet. Mol. Biol.. Publicado em: 28/02/2019