Dna Physical Mapping
Mostrando 1-12 de 375 artigos, teses e dissertações.
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1. Human molecular cytogenetics: from cells to nucleotides
The field of cytogenetics has focused on studying the number, structure, function and origin of chromosomal abnormalities and the evolution of chromosomes. The development of fluorescent molecules that either directly or via an intermediate molecule bind to DNA has led to the development of fluorescent in situ hybridization (FISH), a technology linking cytog
Genet. Mol. Biol.. Publicado em: 2014
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2. Scattered organization of the histone multigene family and transposable elements in Synbranchus
The fish species Synbranchus marmoratus is widely distributed throughout the Neotropical region and exhibits a significant karyotype differentiation. However, data concerning the organization and location of the repetitive DNA sequences in the genomes of these karyomorphs are still lacking. In this study we made a physical mapping of the H3 and H4 histone mu
Genet. Mol. Biol.. Publicado em: 2014
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3. Mining plant genome browsers as a means for efficient connection of physical, genetic and cytogenetic mapping: an example using soybean
Physical maps are important tools to uncover general chromosome structure as well as to compare different plant lineages and species, helping to elucidate genome structure, evolution and possibilities regarding synteny and colinearity. The increasing production of sequence data has opened an opportunity to link information from mapping studies to the underly
Genetics and Molecular Biology. Publicado em: 2012
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4. Karyotypic conservatism in samples of Characidium cf. zebra (Teleostei, Characiformes, Crenuchidae): physical mapping of ribosomal genes and natural triploidy
Basic and molecular cytogenetic analyses were performed in specimens of Characidium cf. zebra from five collection sites located throughout the Tietê, Paranapanema and Paraguay river basins. The diploid number in specimens from all samples was 2n = 50 with a karyotype composed of 32 metacentric and 18 submetacentric chromosomes in both males and females. Co
Genetics and Molecular Biology. Publicado em: 25/03/2011
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5. Resistência à ferrugem do cafeeiro: mapeamento genético, físico e análise da expressão gênica em resposta a infecção de H. vastatrix / Coffea leaf rust: genetic, physical mapping and analysis of the gene expression in response to infection the H. vastatrix
The coffee leaf rust it is caused by the biothrofic fungus Hemileia vastatrix is one diseases that more it causes economical damages. The coffee breeding for obtaining for resistance plants it is slow and the moleculars studies of identification for resistance genes to H. vastatrix are still few informative. This study had as objective to obtain information
Publicado em: 2009
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6. Physical mapping of the 18S and 5S ribosomal genes in nine Characidae species (Teleostei, Characiformes)
Characidae is one of the largest fish families of the Neotropical region, and presenting a pronounced morphological variability, certainly does not constitute a monophyletic group. The cytogenetical data also show a large chromosomal variation and can provide important information for a better understanding of the relationships between the species of this gr
Genetics and Molecular Biology. Publicado em: 2008
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7. Karyotype evolution of Crotalaria (L.) species of the Hedriocarpae section, Macrostachyae subsection (Leguminosae-Papilionoideae) / Evolução cromossômica de espécies de Crotalaria (L.) da seção Hedriocarpae, subseção Macrostachyae (Leguminosae-Papilionoideae)
Approximately 600 species of the Genus Crotalaria have been described, the majority is located in tropical and subtropical regions. These species are classified into eight botanical sections and nine subsections according to floral characteristics. The botanical sections can be divided into two major groups: one with species presenting flower specialization
Publicado em: 2008
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8. Analysis and chromosomal mapping of Leishmania (Leishmania) amazonensis amastigote expressed sequence tags
The characterization of expressed sequence tags (ESTs) generated from a cDNA library of Leishmania (Leishmania) amazonensis amastigotes is described. The sequencing of 93 clones generated new L. (L.) amazonensis ESTs from which 32% are not related to any other sequences in database and 68% presented significant similarities to known genes. The chromosome loc
Memórias do Instituto Oswaldo Cruz. Publicado em: 05/07/2007
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9. O problema dos uns consecutivos utilizando arquiteturas reconfiguráveis
Reconfigurable architectures enable the hardware function to be implemented by the user. Due to its characteristics, these architectures have been used in many areas, including Bioinformatics. Many problems in Bioinformatics can be represented by mathematical models that, in turn, can be solved by computational methods. The consecutive ones problems is an ex
Publicado em: 2007
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10. Karyotype characterization of Crotalaria juncea (L.) by chromosome banding and physical mapping of 18S-5.8S-26S and 5S rRNA gene sites
The chromosomes of Crotalaria juncea, a legume of agronomic interest with a 2n = 16 karyotype composed of metacentric chromosomes, were analyzed using several cytogenetic techniques. C-banding revealed heterochromatic regions around the centromeres in all chromosomes and adjacent to the secondary constriction on the chromosome 1 short arm. Fluorescent staini
Genetics and Molecular Biology. Publicado em: 2007
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11. Origem e diferenciação do sistema de cromossomos sexuais ZZ/ZW em Triportheus (Characiformes, Characidae): citogenética, mapeamento dos genes ribossomais e microdissecção cromossômica
Chromosomes of distinct species of Triportheus (Characiformes, Characidae) from different hydrographic basins in Brazil, as well as in Argentina, were analyzed in the present work. Triportheus nematurus, from Piracicaba River (São Paulo State, Brazil), was analyzed for the first time, using conventional cytogenetic techniques, base-specific fluorochromes an
Publicado em: 2007
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12. Karyotype differentiation of four Cestrum species (Solanaceae) based on the physical mapping of repetitive DNA
We studied the karyotypes of four Brazilian Cestrum species (C. amictum, C. intermedium, C. sendtnerianum and C. strigilatum) using conventional Feulgen staining, C-Giemsa and C-CMA3/DAPI banding, induction of cold-sensitive regions (CSRs) and fluorescent in situ hybridization (FISH) with rDNA probes. We found that the karyotypes of all four species was 2n =
Genetics and Molecular Biology. Publicado em: 2006