Dna Hybridization Probes
Mostrando 1-12 de 1710 artigos, teses e dissertações.
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1. [PROVISIONAL] Low cytomolecular diversification in the genus Stylosanthes Sw. (Papilionoideae, Leguminosae)
Abstract Stylosanthes (Papilionoideae, Leguminosae) is a predominantly neotropical genus with ~ 48 species that includes worldwide important forage species. This study presents the chromosome number and morphology of eight species of the genus Stylosanthes (S. acuminata, S. gracilis, S. grandifolia, S. guianensis, S. hippocampoides, S. pilosa, S. macrocephal
Genet. Mol. Biol.. Publicado em: 19/08/2019
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2. Validation of chromogenic in situ hybridization reactions for DNA and RNA detection in formalin-fixed paraffin-embedded tissue
RESUMO Introdução: A hibridização in situ cromogênica (CISH) é uma alternativa aos métodos tradicionais imuno-histoquímicospara diagnóstico de doenças infecciosas em amostras fixadas em formalina e incluídas em parafina, visto que apresenta grande sensibilidade e especificidade. Esse tipo de amostra sofre diversas modificações químicas durante
J. Bras. Patol. Med. Lab.. Publicado em: 09/05/2019
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3. Comparative Cytogenetics Among Three Sympatric Hypostomus Species (Siluriformes: Loricariidae): An Evolutionary Analysis in a High Endemic Region
ABSTRACT The karyotypes of three armored catfish species (Loricariidae) from the Iguaçu river, southern of the Brazil, were compared using different techniques: C-banding, Ag-NOR and fluorescence in situ hybridization (FISH), which used 5S and 18S rDNAs and total Cot-1 fraction as probes. Hypostomus commersoni and Hypostomus derbyi presented 2n = 68 chromos
Braz. arch. biol. technol.. Publicado em: 14/11/2018
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4. Karyotype and genome size comparative analyses among six species of the oilseed-bearing genus Jatropha (Euphorbiaceae)
Abstract Jatropha is an important genus of Euphorbiaceae, with species largely used for various purposes, including the manufacturing of soaps and pharmaceutical products and applications in the bioenergetic industry. Although there have been several studies focusing J. curcas in various aspects, the karyotype features of Jatropha species are poorly known. T
Genet. Mol. Biol.. Publicado em: 14/05/2018
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5. Comparative cytogenetic analysis in Erythrolamprus snakes (Serpentes: Dipsadidae) from Argentina
ABSTRACT We described the karyotypes of five snake taxa from Argentina: Erythrolamprus almadensis, E. ceii, E. poecilogyrus caesius, E. p. schotti and E. p. sublineatus, and also intergrading individuals between the last two subspecies by conventional staining, chromosome bandings and fluorescent in situ hybridization (FISH) with 28S ribosomal DNA probes. Er
An. Acad. Bras. Ciênc.. Publicado em: 2018-04
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6. Evolution of satellite DNA sequences in two tribes of Bovidae: A cautionary tale
Abstract Two clones, Bt1 from Bos taurus and Om1 from Ovis orientalis musimon, were used as probes for hybridization on genomic DNA and on metaphase chromosomes in members of Bovini and Caprini tribes. Bt1 and Om1 are sequences respectively belonging to the 1.715 and 1.714 DNA satellite I families. Southern blots and fluorescence in situ hybridization experi
Genet. Mol. Biol.. Publicado em: 24/11/2015
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7. Differential detection of transposable elements between Saccharum species
Cultivars of sugarcane (Saccharum) are hybrids between species S. officinarum (x = 10, 2n = 8x = 80) and S. spontaneum (x=8,2n=5-16x =40-128). These accessions have 100 to 130 chromosomes, 80-85% of which are derived from S. officinarum, 10-15% from S. spontaneum, and 5-10% are possible recombinants between the two genomes. The aim of this study was to analy
Genet. Mol. Biol.. Publicado em: 16/08/2013
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8. Karyotypic conservatism in five species of Prochilodus (Characiformes, Prochilodontidae) disclosed by cytogenetic markers
The family Prochilodontidae is considered a group with well conserved chromosomes characterized by their number, morphology and banding patterns. Thence, our study aimed at accomplishing a cytogenetic analysis with conventional methods (Giemsa staining, silver staining of the nucleolus organizer regions-AgNOR, and C-banding) and fluorescence in situ hybridiz
Genet. Mol. Biol.. Publicado em: 2013
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9. Citogenética comparativa em espécies de Hypostomus (Siluriformes, Loricariidae, Hypostominae) : contribuição da fração repetitiva do genoma para a diversidade cromossômica do grupo
Hypostomus is the widest genus of the armored catfishes in South America. This group presents a great diversity in color pattern and morphology, and shows not conservative chromosomal features. Different classes of repetitive DNAs have distinct evolutionary dynamics in the genome of eukaryotes. Therefore, analyses and distribution of these sequences in chrom
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 14/11/2012
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10. Impacto da presenÃa de atrazina na comunidade bacteriana do solo / Impact of atrazine on bacteriological soil community
Chemical contamination removal in soil and water depends on microbiological community that is able to degrade these compounds. There is a great evolutionary interest on studying microorganisms that metabolize the xenobiotic ones, since they have relatively been seen as new in the last five decades. Little is known about structure variation of microbiological
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 13/02/2012
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11. Mining plant genome browsers as a means for efficient connection of physical, genetic and cytogenetic mapping: an example using soybean
Physical maps are important tools to uncover general chromosome structure as well as to compare different plant lineages and species, helping to elucidate genome structure, evolution and possibilities regarding synteny and colinearity. The increasing production of sequence data has opened an opportunity to link information from mapping studies to the underly
Genetics and Molecular Biology. Publicado em: 2012
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12. Rapid identification of Mycobacterium tuberculosis complex by a novel hybridization signal amplification method based on self-assembly of DNA-streptavidin nanoparticles
Rapid detection of Mycobacterium tuberculosis complex (MTBC) is a critical step in controlling tuberculosis (TB). In this study, we used IS6110 as the specific identification target to develop a novel hybridization signal amplification method (HSAM) for the rapid and direct detection of MTBC from clinical sputum specimens. This system consists of magnetic be
Brazilian Journal of Microbiology. Publicado em: 2011-09