Dna Heteroduplex
Mostrando 1-12 de 557 artigos, teses e dissertações.
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1. Gene rearrangement study for minimal residual disease monitoring in children with acute lymphocytic leukemia
OBJECTIVE To detect markers for minimal residual disease monitoring based on conventional polymerase chain reaction for immunoglobulin, T-cell receptor rearrangements and the Sil-Tal1 deletion in patients with acute lymphocytic leukemia. METHODS Fifty-nine children with acute lymphocytic leukemia from three institutions in Minas Gerais, Brazil, were pros
Rev. Bras. Hematol. Hemoter.. Publicado em: 2013
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2. High frequency of potentially pathogenic yeast species in goat’s raw milk and creamed cheese in Southern Brazil = Alta frequência de leveduras potencialmente patogênicas no leite de cabra in natura e no queijo de cabra cremoso no Sul do Brasil / Alta frequência de leveduras potencialmente patogênicas no leite de cabra in natura e no queijo de cabra cremoso no Sul do Brasil
Existem poucos relatos sobre isolamento, contagem e identificação de leveduras no leite de cabra in natura e derivados. O objetivo desse estudo foi avaliar a diversidade de leveduras encontradas no leite de cabra cru e no queijo cremoso de cabra coletados na região metropolitana de Porto Alegre, Brasil. Foi desenvolvida a técnica de HMA (Heteroduplex Mob
Publicado em: 2011
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3. Analysis of the SLC4A1 gene in three Mexican patients with hereditary spherocytosis: report of a novel mutation
We analyzed the SLC4A1 gene in three Mexican patients with Hereditary Spherocytosis (HS). The promoter and all 20 exons were investigated through heteroduplex analysis and DNA sequencing. No DNA changes were detected in one of the three patients. Two well-known polymorphisms, Memphis I and the Diego-a blood group, were detected in another one. In the third,
Genetics and Molecular Biology. Publicado em: 18/12/2009
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4. INFLUÊNCIA DE BARREIRAS GEOGRÁFICAS NA ESTRUTURA GENÉTICA DE POPULAÇÕES DE Aegla uruguayana Schmitt, 1942 (Crustacea, Decapoda, Anomura) / INFLUENCE OF GEOGRAPHIC BARRIERS IN THE GENETIC STRUCTURE OF Aegla uruguayana Schmitt, 1942 (Crustacea, Decapoda, Anomura) POPULATIONS
Muitas espécies tiveram populações separadas por barreiras geográficas milhões de anos atrás durante a formação dos sistemas de drenagem dos Rios da América do Sul. Modificações no relevo levaram a atual formação das bacias hidrográficas do estado do Rio Grande do Sul. Como estas bacias não possuem ligação entre si, em espécies com distribu
Publicado em: 2007
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5. Serotyping HIV-1 with V3 peptides: detection of high avidity antibodies presenting clade-specific reactivity
The main objective of the present study was to assess the specificity and sensitivity of a modified assay using short synthetic peptides of the V3 region of HIV-1 gp120, which is the main target for neutralizing antibodies. Results from an enzyme immunoassay (EIA) employing a panel of synthetic peptides of HIV-1 subtypes and using urea washes to detect high
Brazilian Journal of Medical and Biological Research. Publicado em: 2002-03
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6. HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil
In order to assess the molecular epidemiology of HIV-1 in two neighboring cities located near the epicenter of the HIV-1 epidemics in Brazil (Santos and São Paulo), we investigated 83 HIV-1 strains obtained from samples collected in 1995 from intravenous drug users. The V3 through V5 region of the envelope of gp 120 was analyzed by heteroduplex mobility ana
Brazilian Journal of Medical and Biological Research. Publicado em: 2001-01
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7. Alterações proteicas da membrana eritrocitaria na eliptocitose hereditaria e "Eliptocitose Adquirida"
No presente trabalho foram analisados 10 pacientes com EIH e seus familiares através do estudo das proteínas totais em SDS-P AGE (Sistema Fairbanks 3,5-17% e Sistema Laemmli 12%), estudo da auto-associação de heterodímeros de espectrina e digestão tríptica da espectrina. Seis destas famílias apresentaram defeito na auto-associação dos heterodímero
Publicado em: 1994
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8. Heterology of mitochondrial DNA from mammals detected by electron microscopic heteroduplex analyses.
Heteroduplex analysis of mitochondrial DNA (mtDNA) from evolutionary closely related mammals (rat vs. mouse, man vs. monkey) are analyzed and compared to heteroduplex analysis of mt-DNA from more distantly related mammals (rat vs. man, rat vs. monkey, mouse vs. man, mouse vs. monkey and man vs. cow). Each analysis is transformed into a heteroduplex map and a
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9. Unrepaired Heteroduplex DNA in Saccharomyces Cerevisiae Is Decreased in Rad1 Rad52-Independent Recombination
A direct repeat recombination assay between SUP4 heteroalleles detects unrepaired heteroduplex DNA (hDNA) as sectored colonies. The frequency of unrepaired heteroduplex is dependent on the mismatch and is highest in a construct that generates C:C or G:G mispairs and lowest in one that generates T:G or C:A mispairs. In addition, unrepaired hDNA increases for
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10. Heteroduplex DNA Formation Is Associated with Replication and Recombination in Poxvirus-Infected Cells
Poxviruses are large DNA viruses that replicate in the cytoplasm of infected cells and recombine at high frequencies. Calcium phosphate precipitates were used to cotransfect Shope fibroma virus-infected cells with different DNA substrates and the recombinant products assayed by genetic and biochemical methods. We have shown previously that bacteriophage lamb
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11. Physical detection of heteroduplexes during meiotic recombination in the yeast Saccharomyces cerevisiae.
We describe a general physical method for detecting the heteroduplex DNA that is formed as an intermediate in meiotic recombination in the yeast Saccharomyces cerevisiae. We use this method to study the kinetic relationship between the formation of heteroduplex DNA and other meiotic events. We show that strains with the rad50, but not the rad52, mutation are
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12. Mismatch repair in heteroduplex DNA.
DNA with base pair mismatches was prepared by annealing mixtures of genetically marked DNA from bacteriophage lambda. This heteroduplex DNA was used to transfect bacteria under conditions minimizing recombination. Genetic analysis of the progeny phages indicates that: (i) Mismatch repair occurs, usually giving rise to a DNA molecule with one chain with the g