Dna Biosensor
Mostrando 13-24 de 32 artigos, teses e dissertações.
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13. Desenvolvimento de biossensores eletroquimicos como ferramenta para avaliação da capacidade antioxidante de extratos vegetais / Development of electrochemical biosensors as a tool for evaluation of the antioxidant capacity of vegetables extracts
This work describes the development of two kind of electrochemical biosensors to evaluate the antioxidant capacity of vegetables extracts. The first biosensor developed was based on horseradish peroxidase immobilized on silica coated with titanium oxide and this material was employed to modify the carbon paste. In the optimized conditions, the biosensor resp
Publicado em: 2005
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14. A fiber optic biosensor for fluorimetric detection of triple-helical DNA.
A fiber optic biosensor was used for the fluorimetric detection of T/AT triple-helical DNA formation. The surfaces of two sets of fused silica optical fibers were functionalized with hexaethylene oxide linkers from which decaadenylic acid oligonucleotides were grown in the 3'to 5'and 5'to 3'direction, respectively, using a DNA synthesizer. Fluorescence studi
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15. Purification of a ligand for the EPH-like receptor HEK using a biosensor-based affinity detection approach.
Advances in screening technologies allowing the identification of growth factor receptors solely by virtue of DNA or protein sequence comparison call for novel methods to isolate corresponding ligand growth factors. The EPH-like receptor tyrosine kinase (RTK) HEK (human EPH-like kinase) was identified previously as a membrane antigen on the LK63 human pre-B-
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16. Studies of temperature-dependent electronic transduction on DNA hairpin loop sensor
A self-assembly monolayer (SAM) of hairpin DNA can be formed on a gold substrate in order to make special biosensors. Labeling the hairpin loop probes with electroactive compositions rather than a fluorophore illustrates interesting profiles of redox current versus temperature. For a biosensor interacting with perfectly complementary targets, the profile sho
Oxford University Press.
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17. Detection of DNA damage by use of Escherichia coli carrying recA'::lux, uvrA'::lux, or alkA'::lux reporter plasmids.
Plasmids were constructed in which DNA damage-inducible promoters recA, uvrA, and alkA from Escherichia coli were fused to the Vibrio fischeri luxCDABE operon. Introduction of these plasmids into E. coli allowed the detection of a dose-dependent response to DNA-damaging agents, such as mitomycin and UV irradiation. Bioluminescence was measured in real time o
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18. Submicron patterning of DNA oligonucleotides on silicon
The covalent attachment of DNA oligonucleotides onto crystalline silicon (100) surfaces, in patterns with submicron features, in a straightforward, two-step process is presented. UV light exposure of a hydrogen-terminated silicon (100) surface coated with alkenes functionalized with N-hydroxysuccinimide ester groups resulted in the covalent attachment of the
Oxford University Press.
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19. Single-nucleotide polymorphism genotyping on optical thin-film biosensor chips
Single-nucleotide polymorphisms (SNPs) constitute the bulk of human genetic variation and provide excellent markers to identify genetic factors contributing to complex disease susceptibility. A rapid, sensitive, and inexpensive assay is important for large-scale SNP scoring. Here we report the development of a multiplex SNP detection system using silicon chi
National Academy of Sciences.
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20. Engineering a signal transduction mechanism for protein-based biosensors
Hybridization-induced conformational changes have been successfully used in biosensors for the transduction of DNA-binding events into readily observable optical or electronic signals. Similar signal transduction has not, however, proven of equal utility in proteinbased biosensors. The discrepancy arises because, unlike ssDNA, most proteins do not undergo si
National Academy of Sciences.
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21. Energetic basis for selective recognition of T·G mismatched base pairs in DNA by imidazole-rich polyamides
To complement available structure and binding results and to develop a detailed understanding of the basis for selective molecular recognition of T·G mismatches in DNA by imidazole containing polyamides, a full thermodynamic profile for formation of the T·G–polyamide complex has been determined. The amide-linked heterocycles f-ImImIm and f-PyImIm (where
Oxford University Press.
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22. Detection of methylation of human p16Ink4a gene 5′-CpG islands by electrochemical method coupled with linker-PCR
Aberrant DNA methylation of the CpG site is among the earliest and most frequent alterations in cancer. Detection of promoter hypermethylation of cancer-related genes may be useful for cancer diagnosis or the detection of recurrence. p16, an inhibitor of the cyclin D-dependent protein kinases, is a classical tumor suppressor gene, and its inactivation is clo
Oxford University Press.
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23. The salt dependence of DNA recognition by NF-kappaB p50: a detailed kinetic analysis of the effects on affinityand specificity.
The binding kinetics of NF-kappaB p50 to the Ig-kappaB site and to a DNA duplex with no specific binding site were determined under varying conditions of potassium chloride concentration using a surface plasmonresonance biosensor. Association and dissociation rate constants were measured enabling calculation of the dissociation constants. Under previously es
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24. Oligonucleotide hybridization studied by a surface plasmon diffraction sensor (SPDS)
A novel label-free biosensor concept based on surface plasmon-enhanced diffraction by micro- patterned interfaces was applied to the study of hybridization reactions of target DNA oligonucleotides (15mers and 75mers) from solution to probe DNA oligonucleotides attached via streptavidin to the sensor surface. The self-referencing and quadratic signal amplific
Oxford University Press.