Dna Amounts
Mostrando 1-12 de 2333 artigos, teses e dissertações.
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1. DNA repair genes in astrocytoma tumorigenesis, progression and therapy resistance
Abstract Glioblastoma (GBM) is the most common and malignant type of primary brain tumor, showing rapid development and resistance to therapies. On average, patients survive 14.6 months after diagnosis and less than 5% survive five years or more. Several pieces of evidence have suggested that the DNA damage signaling and repair activities are directly correl
Genet. Mol. Biol.. Publicado em: 13/12/2019
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2. Preanalytical conditions can interfere with M. tuberculosis detection by PCR in respiratory samples
OBJECTIVES: Tuberculosis is one of the most prevalent infections in humans. Although culture is the reference for diagnosis, its sensitivity is compromised, especially in paucibacillary samples. Because polymerase chain reaction (PCR) amplifies mycobacterial DNA, it is more sensitive than culture for the diagnosis of Mycobacterium tuberculosis (Mtb). Howeve
Clinics. Publicado em: 29/11/2018
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3. Decellularization of placentas: establishing a protocol
Biological biomaterials for tissue engineering purposes can be produced through tissue and/or organ decellularization. The remaining extracellular matrix (ECM) must be acellular and preserve its proteins and physical features. Placentas are organs of great interest because they are discarded after birth and present large amounts of ECM. Protocols for decellu
Braz J Med Biol Res. Publicado em: 17/11/2017
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4. The agricultural impact of pesticides on Physalaemus cuvieri tadpoles (Amphibia: Anura) ascertained by comet assay
ABSTRACT Amphibians inhabiting agricultural areas are constantly exposed to large amounts of chemicals, which reach the aquatic environment during the rainy season through runoff, drainage, and leaching. We performed a comet assay on the erythrocytes of tadpoles found in the surroundings of agricultural fields (soybean and corn crops), where there is an inte
Zoologia (Curitiba). Publicado em: 23/10/2017
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5. Assessment of Rice Associated Bacterial Ability to Enhance Rice Seed Germination and Rice Growth Promotion
ABSTRACT The application of beneficial bacteria has recently been used for sustainable agriculture. In current research, 71 bacterial isolates were obtained from rice plant and the rhizosphere soil of different paddy fields in Guilan province, Iran. After primitive investigation, 40 bacteria with typical predominant characteristics were selected. By PCR-RFLP
Braz. arch. biol. technol.. Publicado em: 17/08/2017
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6. Evaluation of the association between the TAS1R2 and TAS1R3 variants and food intake and nutritional status in children
Abstract Taste perception plays a key role in determining individual food preferences and dietary habits and may influence nutritional status. This study aimed to investigate the association of TAS1R2 (Ile191Val - rs35874116) and TAS1R3 (-1266 C/T - rs35744813) variants with food intake and nutritional status in children followed from birth until 7.7 years o
Genet. Mol. Biol.. Publicado em: 11/05/2017
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7. Genetic Variability and Symbiotic Efficiency of Erythrina velutina Willd. Root Nodule Bacteria from the Semi-Arid Region in Northeastern Brazil
ABSTRACT Legume-rhizobia symbiosis is a cross-kingdom association that results in large amounts of nitrogen incorporated in food webs. For the Brazilian semi-arid region, data on genetic variability and symbiotic efficiency of Papilionoidae rhizobial communities are very scarce. The aim of this study was to evaluate the genetic variability and the symbiotic
Rev. Bras. Ciênc. Solo. Publicado em: 06/02/2017
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8. Sugarcane trash levels in soil affects the fungi but not bacteria in a short-term field experiment
Abstract The sugarcane in Brazil is passing through a management transition that is leading to the abolition of pre-harvest burning. Without burning, large amounts of sugarcane trash is generated, and there is a discussion regarding the utilization of this biomass in the industry versus keeping it in the field to improve soil quality. To study the effects of
Braz. J. Microbiol.. Publicado em: 2016-06
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9. Genetic identification of bucktooth parrotfish Sparisoma radians (Valenciennes, 1840) (Labridae, Scarinae) by chromosomal and molecular markers
Parrotfishes (Labridae, Scarinae) comprise a large marine fish group of difficult identification, particularly during juvenile phase when the typical morphology and coloration of adults are absent. Therefore, the goal of this study was to test cytogenetic markers and DNA barcoding in the identification of bucktooth parrtotfish Sparisoma radians from the nort
Genet. Mol. Biol.. Publicado em: 14/11/2014
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10. Development of a Taqman real-time PCR assay for detection of Leifsonia xyli subsp xyli
Leifsonia xyli subsp xyli (Lxx) is the etiological agent of ratoon-stunting disease (RSD), one of the most important diseases that affects productivity of sugarcane crops. The lack of defined external symptoms in most plants is a challenge for the diagnosis of RSD facilitating its spread in production fields. In this study a Taqman® real-time polymerase cha
Trop. plant pathol.. Publicado em: 09/07/2013
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11. Plasmid-based controls to detect rpoB mutations in Mycobacterium tuberculosis by quantitative polymerase chain reaction-high-resolution melting
Quantitative polymerase chain reaction-high-resolution melting (qPCR-HRM) analysis was used to screen for mutations related to drug resistance in Mycobacterium tuberculosis. We detected the C526T and C531T mutations in the rifampicin resistance-determining region (RRDR) of the rpoB gene with qPCR-HRM using plasmid-based controls. A segment of the RRDR region
Mem. Inst. Oswaldo Cruz. Publicado em: 2013-02
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12. Desenvolvimento de processo microfluídico para incorporação de DNA em lipossomas catiônicos destinados a terapia e vacinação gênica = : Development of microfluidic process for DNA incorporation into cationic liposomes for gene therapy and vaccination / Development of microfluidic process for DNA incorporation into cationic liposomes for gene therapy and vaccination
This research aimed at the technological development of microfluidic process for nonviral carriers production based on the electrostatic complexation between cationic liposomes (CL) and plasmidal DNA (pDNA) for gene and vaccine therapy applications. The development of this process was compared to the conventional bulk process, in which the solutions are mixe
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 22/07/2012