Dmpo
Mostrando 1-12 de 15 artigos, teses e dissertações.
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1. Antioxidant properties of plant extracts: an EPR and DFT comparative study of the reaction with DPPH, TEMPOL and spin trap DMPO
Este trabalho apresenta um estudo comparativo da atividade antioxidante dos extratos de nove espécies de plantas pertencentes à flora brasileira - Swartzia langsdorffii, Machaerium villosum, Pterogyne nitens, Pera glabrata, Aegiphyla sellowiana, Copaifera langsdorffii, Chrysophyllum inornatum, Iryanthera juruensis, Didymopanax venosum - neutralizando os ra
Journal of the Brazilian Chemical Society. Publicado em: 2009
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2. Mecanismos da ação antioxidante dos ácidos caféico e tânico em sistemas contendo íons ferro
Este trabalho foi dividido em três capítulos distintos. No primeiro capítulo, foi feita uma reavaliação da metodologia da degradação oxidativa da 2-desoxirribose (2-DR) em sistemas geradores de radicais livres mediados por íons ferrosos. Este ensaio é amplamente utilizado para avaliar a atividade pró/antioxidante de moléculas isoladas ou extratos
Publicado em: 2009
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3. Estudo da ação antioxidante in vitro dos extratos alcoólicos das folhas e das flores da capuchinha (Tropaeolum majus L.)
Nasturtium (Tropaeolum majus L.) é uma planta ornamental de crescimento rápido nativa do México e Peru. As suas folhas e flores são utilizadas na culinária, como enfeite e devido às qualidades de sabor e pelos seus efeitos medicinais. A extração das folhas e flores frescas foi feita por maceração durante 15 dias em etanol (70%), depois os extratos
Publicado em: 2008
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4. Antioxidant Activity of Plant Extracts from Brazilian Flora: Study of Electron Paramagnetic Resonance (EPR) and Density Functional Theory (DFT). / Atividade antioxidante de extratos vegetais da flora brasileira: estudo com ressonância paramagnética eletrônica (RPE) e teoria do funcional da densidade (TFD)
There is, in Brazil, a great diversity of vegetable species, and a popular knowledge of several medicinal properties of the some of them. In studies carried out with plants? extracts, there is special interest in antioxidant activities. This work, focused in antioxidant activity, is divided in two parts: the first uses EPR technique to study the antioxidant
Publicado em: 2006
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5. Reexamination of the mechanism of hydroxyl radical adducts formed from the reaction between familial amyotrophic lateral sclerosis-associated Cu,Zn superoxide dismutase mutants and H2O2
Amyotrophic lateral sclerosis (ALS) involves the progressive degeneration of motor neurons in the spinal cord and motor cortex. Mutations to Cu,Zn superoxide dismutase (SOD) linked with familial ALS are reported to increase hydroxyl radical adduct formation from hydrogen peroxide as measured by spin trapping with 5,5′-dimethyl-1-pyrrolline N-oxide (DM
The National Academy of Sciences.
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6. Copper, zinc superoxide dismutase catalyzes hydroxyl radical production from hydrogen peroxide.
Cu,Zn superoxide dismutase (Cu,Zn-SOD; EC 1.15.1.1) is known to be inhibited slowly by H2O2. Using EPR and the spin traps 5,5-dimethyl-1-pyrroline 1-oxide (DMPO) and N-tert-butyl-alpha-phenylnitrone (PBN), we have shown that Cu,Zn-SOD catalyzes the formation of "free" .OH radicals from H2O2 in pH 7.6 bicarbonate buffer. Supporting evidence includes the follo
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7. The Radical Trap 5,5-Dimethyl-1-Pyrroline N-Oxide Exerts Dose-Dependent Protection against Myocardial Ischemia-Reperfusion Injury through Preservation of Mitochondrial Electron Transport
Free radicals are important mediators of myocardial ischemia-reperfusion injury. Nitrone spin traps have been shown to scavenge free radicals. The cardioprotective effect of the spin trap, 5,5-dimethyl-1-pyrroline N-oxide (DMPO), was investigated in an isolated heart model of global ischemia and reperfusion. Rat hearts were perfused and subjected to glob
American Society for Pharmacology and Experimental Therapeutics.
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8. Hydroxyl radical generation by polymorphonuclear leukocytes measured by electron spin resonance spectroscopy.
Electron spin resonance spectroscopy using the spin trap 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) was employed to detect the formation of hydroxyl radicals (OH.) by phagocytosing polymorphonuclear leukocytes (PMN). An electron spin resonance signal with the identical g value and splitting characteristics of the DMPO/OH). adduct was detected on incubation of n
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9. Endogenous intracellular glutathionyl radicals are generated in neuroblastoma cells under hydrogen peroxide oxidative stress.
We report the detection of endogenous intracellular glutathionyl (GS.) radicals in the intact neuroblastoma cell line NCB-20 under oxidative stress. Spin-trapping and electron paramagnetic resonance (EPR) spectroscopic methods were used for monitoring the radicals. The cells incubated with the spin trap 5,5-dimethyl-1-pyrroline 1-oxide (DMPO) were challenged
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10. Hyperoxic sheep pulmonary microvascular endothelial cells generate free radicals via mitochondrial electron transport.
Free radical generation by hyperoxic endothelial cells was studied using electron paramagnetic resonance (EPR) spectroscopy and the spin trap 5,5-dimethyl-1-pyrroline-N-oxide (DMPO). Studies were performed to determine the radical species produced, whether mitochondrial electron transport was involved, and the effect of the radical generation on cell mortali
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11. Manganese(II)-bicarbonate-mediated catalytic activity for hydrogen peroxide dismutation and amino acid oxidation: detection of free radical intermediates.
To examine the structural identities of reactive free radicals and the mechanism of the oxidative modification of proteins, we used EPR and spin-trapping methods to investigate the oxidation of amino acids by H2O2 as well as the decomposition of H2O2 itself catalyzed by Mn(II) ions. Superoxide and hydroxyl radicals (O2-. and OH.) were trapped by a spin trap,
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12. Direct measurement of nitroxide pharmacokinetics in isolated hearts situated in a low-frequency electron spin resonance spectrometer: implications for spin trapping and in vivo oxymetry.
The pharmacokinetics of two nitroxides were investigated in isolated rat hearts situated in a low-frequency electron spin resonance spectrometer. The spin labels 2,2,3,3,5,5-hexamethyl-1-pyrrolidinyloxy and 3-carboxy-2,2,5,5-tetramethyl-1-pyrrolidinyloxy were chosen for their physiochemical analogy to the spin trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO) and