Dispersed Generation
Mostrando 13-24 de 37 artigos, teses e dissertações.
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13. Intracellular Pasteurella pseudotuberculosis: Multiplication in Cultured Spleen and Kidney Cells 1
Pasteurella pseudotuberculosis multiplied within rabbit cells in primary culture. Spleen cells from rabbits were either dispersed mechanically, exposed to the organism immediately after cell preparation, and grown as a pellet or the cells were dispersed enzymatically and grown as a monolayer for 4 to 6 days before the bacteria were introduced. Intracellular
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14. A Quantitative Analysis of the Genetics of Resting Blood Lactic Acid Levels in Mice
Resting blood lactate levels were measured in inbred mouse strains, their F1, and several of their segregating generations to determine whether the level of lactic acid is influenced by genetic factors. The inbred strains in each of the two sets used differed significantly from one another for this character. Only one strain showed a significant sex differen
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15. NUTRITIONAL REQUIREMENTS FOR VEGETATIVE GROWTH OF MYXOCOCCUS XANTHUS
Dworkin, Martin (Indiana University Medical Center, Indianapolis, Ind.). Nutritional requirements for vegetative growth of Myxococcus xanthus. J. Bacteriol. 84:250–257. 1962.—This investigation was part of a program to clarify the environmental regulation of fruiting-body formation in a fruiting myxobacterium. By use of a dispersed-growing strain of Myxo
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16. Specific inhibition by prostaglandins E2 and I2 of histamine-stimulated [14C]aminopyrine accumulation and cyclic adenosine monophosphate generation by isolated canine parietal cells.
The effects of prostaglandins E2 and I2 on accumulation of [14C]aminopyrine and the generation of cyclic AMP by fractions of dispersed canine gastric mucosal cells, enriched in their content of parietal cells, have been studied. The parietal cell content of the fractions was enriched to between 43 and 70% using an elutriator rotor. The accumulation of [14C]a
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17. Effects of temperature, Mg2+ concentration and mismatches on triplet-repeat expansion during DNA replication in vitro.
The human genome contains many simple tandem repeats that are widely dispersed and highly polymorphic. At least one group of simple tandem repeats, the DNA trinucleotide repeats, can dramaticallyexpand in size during transmission from one generation to the next to cause disease by a process known as dynamic mutation. We investigated the ability of trinucleot
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18. An H1 histone gene-specific 5' element and evolution of H1 and H5 genes.
In previous studies we have shown that the H5 gene is not closely linked to the dispersed clusters of core and H1 histone genes. Here we emphasise features of H1 and H5 genes relevant to their expression in the chicken genome. Of particular note is an H1 gene-specific 5' element, 5' AAACACA 3' found upstream of all H1 genes studied to date. This "H1-box" is
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19. The var genes of Plasmodium falciparum are located in the subtelomeric region of most chromosomes.
PfEMP1, a Plasmodium falciparum-encoded protein on the surface of infected erythrocytes is a ligand that mediates binding to receptors on endothelial cells. The PfEMP1 protein, which is encoded by the large var gene family, shows antigenic variation and changes in binding phenotype associated with alterations in antigenicity. We have constructed a yeast arti
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20. A Chlamydomonas Genomic Library in Yeast Artificial Chromosomes
We have constructed and characterized a Chlamydomonas reinhardtii total genomic library in yeast artificial chromosomes (YACs). The library contains 7500 clones with inserts ranging in size from 100-200 kb. The representation of the library was assessed by screening one-third of it with a probe derived from the dispersed repeat, Gulliver, which occurs ~13 ti
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21. Hydrogen peroxide generation by polymorphonuclear leukocytes exposed to peritoneal dialysis effluent.
In the presence of peritoneal dialysis effluent (PDE), human polymorphonuclear leukocytes (PMN) showed reduced production of hydrogen peroxide and hypochlorous acid (H2O2 and HOCl, respectively) when at rest and when stimulated with both soluble (formylmethionyl-leucyl-phenylalanine and phorbol myristate acetate) and particulate (Staphylococcus epidermidis)
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22. Hyperactive recombination in the mitochondrial DNA of the natural death nuclear mutant of Neurospora crassa.
In Neurospora crassa, a recessive mutant allele of a nuclear gene, nd (natural death), causes rapid degeneration of the mitochondrial DNA, a process that is manifested phenotypically as an accelerated form of senescence in growing and stationary mycelia. To examine the mechanisms that are involved in the degradation of the mitochondrial chromosome, several m
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23. Combinatorial generation of variable fusion proteins in the Ewing family of tumours.
Balanced translocations involving band q12 of human chromosome 22 are the most frequent recurrent translocations observed in human solid tumours. It has been shown recently that this region encodes EWS, a protein with an RNA binding homologous domain. In Ewing's sarcoma and malignant melanoma of soft parts, translocations of band 22q12 to chromosome 11 and 1
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24. Characterization of Bacteria by Particle Beam Mass Spectrometry
A technique is described for detecting and characterizing bacteria on a single-particle basis by mass spectrometry. The method involves generation of a particle beam of single whole cells which are rapidly volatilized and ionized in vacuum in the ion source of a quadrupole mass spectrometer. The particle beam can be generated, with minimal sample handling, f