Dimethyl Formamide
Mostrando 13-21 de 21 artigos, teses e dissertações.
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13. Effects of aggregation and solvent on the toxicity of amphotericin B to human erythrocytes.
In aqueous suspensions of amphotericin B (AmB), a polyene antibiotic and antifungal agent, three forms of AmB coexist: monomers, water-soluble oligomers, and non-water-soluble aggregates. The toxicity of the water-soluble self-associated form of AmB compared with that of the non-water-soluble self-associated form was tested by measuring induction of K+ leaka
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14. Helix stability and the mechanism of cruciform extrusion in supercoiled DNA molecules.
The kinetic properties of cruciform extrusion in supercoiled DNA molecules fall into two main classes. C-type cruciforms extrude in the absence of added salt, at relatively low temperatures, with large activation energies, while S-type cruciforms exhibit no extrusion in the absence of salt, and maximal rates at 50 mM NaCl, with activation energies about one
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15. Capsule of Escherichia coli K29: ultrastructural preservation and immunoelectron microscopy.
The polysaccharide capsule of Escherichia coli K29 fully surrounds the microorganism and thus occupies an extracellular space ca. 20 times larger in volume than that of the decapsulated cell. Since more than 95% of the capsule consists of water, dehydration for electron microscopy causes the material to collapse. We describe here a method for embedding the c
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16. Optimizing spotting solutions for increased reproducibility of cDNA microarrays
The ability to extract meaningful information from transcriptome technologies such as cDNA microarrays relies on the precision, sensitivity and reproducibility of the measured values for a given gene across multiple samples. Given the lack of a ‘gold standard’ for the production of microarrays using current technologies, there is a high degree of variati
Oxford University Press.
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17. Expression of the X-CGD gene during induced differentiation of myeloid leukemia cell line HL-60.
The expression of the X-CGD gene, which encodes the heavy-chain subunit of the phagocyte cytochrome b, was studied during induced myeloid differentiation of HL-60 cells. Incubation of the cells with a combined regimen of retinoic acid and dimethyl formamide resulted in granulocytic morphological differentiation and acquisition of nitroblue tetrazolium reduct
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18. Alteration of Acrylonitrile-Methylacrylate-Butadiene Terpolymer by Nocardia rhodochrous and Penicillium notatum†
[14C]Barex-210, a terpolymer of acrylonitrile, methylacrylate, and butadiene, was tested for bioconversion. Powdered samples of polymer, each specifically 14C labeled at different carbon atoms of the polymer, were incubated with either Nocardia rhodochrous or Penicillium notatum in an enriched growth medium for various periods of time. After 6 months of incu
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19. Solubilization of the Cytoplasmic Membrane of Escherichia coli by Triton X-100
Treatment of a partially purified preparation of cell walls of Escherichia coli with Triton X-100 at 23 C resulted in a solubilization of 15 to 25% of the protein. Examination of the Triton-insoluble material by electron microscopy indicated that the characteristic morphology of the cell wall was not affected by the Triton extraction. Contaminating fragments
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20. Monoclonal antibodies to human estrogen receptor.
Extranuclear estrogen receptor protein (estrophilin) of MCF-7 human breast cancer cells was purified by passage of the cytosol fraction of a cell homogenate through an affinity column of estradiol linked to Sepharose by a substituted di-n-propyl sulfide bridge in the 17 alpha position. Elution with 50 micro M [3H]estradiol in 10% (vol/vol) dimethyl formamide
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21. Identification of a population of bipotent stem cells in the HL60 human promyelocytic leukemia cell line
HL60, the human promyelocytic leukemia cell line, consists of cells that resemble promyelocytes but are transformed into more mature myeloid forms (polymorphonuclear leukocytes) that express most of the functional characteristics of terminally differentiated myeloid forms when incubated in the presence of dimethyl formamide (HCONMe2). When HL60 cells are exp