Dihydrorhodamine
Mostrando 1-12 de 24 artigos, teses e dissertações.
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1. Endothelial Progenitor Cells and NADPH Oxidase Enzyme Activity in the Development of an Aortic Aneurysm
Abstract Introduction: Endothelial progenitor cells (EPCs) and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase enzyme activity may affect the vessel wall and have a role in development of aortic aneurysms. EPCs originate from hematopoietic stem cells and can be enumerated from peripheral blood samples by flow cytometry. In this study, we aimed t
Brazilian Journal of Cardiovascular Surgery. Publicado em: 2022
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2. Danos oxidativos promovidos por espécies de Mn(III) sobre biomoléculas e células em situação de estresse / Oxidative damage induced by Mn(III) species over biomolecules and stressed cells
Manganese is an essential trace element, however there is considerable concern regarding its neurological effects when in excess, giving rise to a condition termed manganism which is characterized by Parkinson disease-like symptoms. Most evaluations of manganese toxicity use poorly defined Mn(II) species, although Mn(III) is known to accumulate preferentiall
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 08/03/2012
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3. Dihydrorhodamine immobilization onto gold subtrates and its potential application in free radical detection / Imobilização de Dihidrorodamina 1,2,3 em superfície de ouro, com potencial aplicação na detecção de radicais livres
Sensores fluoresecentes são frequentemente usados na detecção de radicais livres. Entre eles, a dihidrorodamina 1,2,3 ( DHR 1,2,3), é um dos mais usados. Neste trabalho, é proposta a imobilização da DHR 1,2,3 em substratos de ouro e sua caracterização como sensor eletroquímico para detecção e quantificação de radicais livres. Os eletrodos foram
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 10/12/2009
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4. Chemiluminescent and flow cytometric analysis of gamma interferon preincubation on neonatal and adult rat polymorphonuclear leukocytes.
Gamma interferon (IFN-gamma) has multiple immunomodulating effects and has been postulated as a possible immunopotentiating agent for the prevention or treatment of neonatal infections. This report describes the effect of rat recombinant IFN-gamma on the oxidative burst activity and CD11b expression of neonatal and adult rat polymorphonuclear leukocytes (PMN
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5. Direct evidence for tumor necrosis factor-induced mitochondrial reactive oxygen intermediates and their involvement in cytotoxicity.
Tumor necrosis factor (TNF) is selectively cytotoxic to some types of tumor cells in vitro and exerts antitumor activity in vivo. Reactive oxygen intermediates (ROIs) have been implicated in the direct cytotoxic activity of TNF. By using confocal microscopy, flow cytometry, and the ROI-specific probe dihydrorhodamine 123, we directly demonstrate that intrace
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6. Memory landscapes of single-enzyme molecules
Immobilized single horseradish peroxidase enzymes were observed by confocal fluorescence spectroscopy during catalysis of the oxidation reaction of the nonfluorescent dihydrorhodamine 6G substrate into the highly fluorescent product rhodamine 6G. By extracting only the non-Markovian behavior of the spectroscopic two-state process of enzyme-product complex fo
The National Academy of Sciences.
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7. Uptake of fluorescent dyes associated with the functional expression of the cystic fibrosis transmembrane conductance regulator in epithelial cells.
Specific mutations in the cystic fibrosis transmembrane conductance regulator (CFTR), the most common autosomal recessive fatal genetic disease of Caucasians, result in the loss of epithelial cell adenosine 3',5'-cyclic-monophosphate (cAMP)-stimulated Cl- conductance. We show that the influx of a fluorescent dye, dihydrorhodamine 6G (dR6G), is increased in c
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8. Susceptibility of melanized and nonmelanized Cryptococcus neoformans to the melanin-binding compounds trifluoperazine and chloroquine.
Cryptococcus neoformans is an opportunistic fungal pathogen which becomes heavily melanized in the presence of phenolic substrates such as L-dopa. Various drugs are known to bind to melanin with high affinity, including the antipsychotic agent trifluoperazine and the antimalarial agent chloroquine. We hypothesized that drugs which bind melanin may have diffe
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9. High-dose catecholamine treatment decreases polymorphonuclear leukocyte phagocytic capacity and reactive oxygen production.
Flow cytometry was used to study phagocytic function (uptake of fluorescein isothiocyanate-labeled bacteria) and release of reactive oxygen products (dihydrorhodamine 123 converted to rhodamine 123) following phagocytosis by neutrophil granulocytes of heparinized whole blood treated with adrenaline, noradrenaline, dopamine, dobutamine, or orciprenaline. Redu
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10. Cytofluorometric analysis of chondrotoxicity of fluoroquinolone antimicrobial agents.
To better understand quinolone-related arthropathy, we conceived an experimental ex vivo model using cell cultures of articular chondrocytes issued from pretreated New Zealand White rabbits (NZW). Juvenile (4- to 5-week-old) NZW were orally dosed with ofloxacin or pefloxacin (300 mg/kg of body weight for 1 day) or with pefloxacin (300 mg/kg for 7 days). Adul
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11. Resistance to peroxynitrite in Neisseria gonorrhoeae
Neisseria gonorrhoeae encodes a number of important genes that aid in survival during times of oxidative stress. The same immune cells capable of oxygen-dependent killing mechanisms also have the capacity to generate reactive nitrogen species (RNS) that may function antimicrobially. F62 and eight additional gonococcal strains displayed a high level of resist
Society for General Microbiology.
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12. Effect of cefodizime and ceftriaxone on phagocytic function in patients with severe infections.
Thirty patients with severe bacterial infections were treated with 50 mg of cefodizime per kg of body weight once daily or 50 mg of ceftriaxone per kg once daily for 10 +/- 3 days. The effect of cefodizime and ceftriaxone on the phagocytic capacity and generation of reactive oxygen intermediates after phagocytosis by granulocytes was assessed prior to, durin