Diagnosis Of Legionnaires Disease
Mostrando 1-12 de 18 artigos, teses e dissertações.
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1. Incidência de infecção por Legionella pneumophila em pacientes que internaram no HCPA com pneumonia adquirida na comunidade
Introdução: O diagnóstico microbiológico da infecção por Legionella é complexo, pois a bactéria não é visualizada à coloração de Gram no escarro, e sua cultura não é realizada na maioria dos laboratórios clínicos. A imunofluorescência direta nas secreções respiratórias tem baixa sensibilidade, em torno de 40% e a técnica da “PCR” n�
Publicado em: 2007
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2. Diagnosis of Legionnaires' Disease by Radioimmunoassay of Legionella Antigen in Pleural Fluid
We describe a case of culture-proven Legionnaires' disease (serogroup 1) in which a rapid diagnosis was made by detection of Legionella antigen in pleural fluid by use of the Binax radioimmunoassay for urine.
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3. Immunoglobulin M antibody titers in the diagnosis of Legionnaires disease.
The purpose of this study was to determine whether measurement of immunoglobulin M (IgM) antibodies against Legionella pneumophila serogroup 1 can aid in the diagnosis of Legionnaires disease. On the basis of measurements of antibody levels in 1,942 control sera, we used an IgM titer of 1:256, observed in 2.3% of the controls, as presumptive evidence of Legi
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4. Rapid diagnosis of an outbreak of Legionnaires' disease at Glasgow Royal Infirmary.
In the last three months of 1985 there was an outbreak of legionnaires' disease at Glasgow Royal Infirmary affecting 15 patients and one surgeon; five patients died. Legionnaires' disease was first suspected when a second case of severe nosocomial pneumonia occurred in a high dependency unit. The application of the direct fluorescent antibody test to specime
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5. Rapid Diagnosis of Legionnaires' Disease Using an Immunochromatographic Assay for Legionella pneumophila Serogroup 1 Antigen in Urine during an Outbreak in The Netherlands
A new immunochromatographic assay for rapid qualitative detection of Legionella pneumophila serogroup 1 antigen in urine specimens was used during an outbreak of legionellosis in The Netherlands. The assay seems of the utmost value in providing a rapid diagnosis of Legionnaires' disease in patients with severe community-acquired pneumonia in an outbreak sett
American Society for Microbiology.
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6. Clinical Utility of Urinary Antigen Detection for Diagnosis of Community-Acquired, Travel-Associated, and Nosocomial Legionnaires' Disease
The clinical utility of Legionella urinary antigen assays for the diagnosis of Legionnaires' disease was assessed by using samples from 317 culture-proven cases. The sensitivities of the Binax enzyme immunoassay (EIA) and Biotest EIA were found to be 93.7 and 94.4% for travel-associated infection and 86.5 and 76.0% for community-acquired infection but only 4
American Society for Microbiology.
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7. Rapid presumptive bacteriological diagnosis of Legionnaires disease.
A simple, relatively rapid silver impregnation stain has been found to stain Legionella pneumophila effectively in paraffin-embedded tissue sections while permitting visualization of histological detail. It may also be used to stain the organism in body fluids. The stain is not specific and thus must be confirmed by direct fluorescent-antibody technique or c
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8. Serology of Legionnaires disease: comparison of indirect fluorescent antibody, immune adherence hemagglutination, and indirect hemagglutination tests.
An immune adherence hemagglutination (IAHA) test for the measurement of antibodies to Legionella pneumophila was developed and evaluated for the diagnosis of Legionnaires disease. Its sensitivity was compared to that of the indirect fluorescent antibody (IFA) test and a recently developed indirect hemagglutination (IHA) test. The sensitivity of the three tes
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9. Detection of mip gene by PCR for diagnosis of Legionnaires' disease.
The mip gene of Legionella pneumophila was demonstrated by PCR and probing in paired acute-phase and convalescent-phase sera from five patients with Legionnaires' disease but not in the acute-phase sera of 100 patients with pneumonia that showed no serological evidence of Legionella infection. PCR may help in cases presenting diagnostic difficulty.
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10. Cefamandole-susceptible strains of Legionella pneumophila serogroup 1: implications for diagnosis and utility as an epidemiological marker.
The standard selective Legionella medium that contains cefamandole failed to grow legionella pneumophila serogroup 1, subtype Bellingham, from a sputum sample from a patient with nosocomial Legionnaires' disease; the isolate did grow on a similar selective medium that substitutes vancomycin for cefamandole. Two Bellingham isolates from this patient's hospita
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11. Bronchial lavage and transbronchial lung biopsy in the diagnosis of Legionnaires' disease.
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12. Formalised yolk sac antigen in early diagnosis of Legionnaires' disease caused by Legionella pneumophila serogroup 1.