Diafiltration
Mostrando 1-12 de 14 artigos, teses e dissertações.
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1. DEVELOPMENT OF A NEW PROCESS FOR PURIFICATION OF CAPSULAR POLYSACCHARIDE FROM Streptococcus pneumoniae SEROTYPE 14
Abstract The main virulence factor of Streptococcus pneumoniae is the capsular polysaccharide (PS), which is the antigen of all current vaccines that are prepared with PS purified from serotypes prevalent in the population. In this work, three purification strategies were evaluated and a new process was developed for purification of serotype 14 PS (PS14), re
Braz. J. Chem. Eng.. Publicado em: 2016-09
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2. Desenvolvimento de espessante alimentar para líquidos com valor nutricional agregado, destinados a indivíduos disfágicos / Development of food thickner for liquids with aggregated nutritional value intended for dysphagic individuals
A deglutição é um processo coordenado e extremamente complexo, envolvendo contração e inibição de músculos localizados entre a boca e o estômago. Alterações neste sistema podem gerar disfagia, sinal comum de diversas doenças orgânicas, alterações neurológicas ou doenças neuromusculares, produzindo no paciente dificuldade na mastigação e de
Publicado em: 2009
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3. Estudo do processo de limpeza de membrana ceramica e obtenção de lecitina de soja por ultrafiltração seguida de diafiltração / Cleaning process in ceramic membrane used to soybean oil degumming and lecithin production by diafiltration
One of the objectives of this work was to study the cleaning process of a 0.01mm pore size ceramic membrane. The operational conditions used at eigth ultrafiltration process realized were 40°C of temperature and 1.0bar and 3.4m/s of transmembrane pressure and cross flow velocity. After each ultrafiltration it have been studied the influence of transmembrane
Publicado em: 2007
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4. Reator enzimático de membrana para proteólise de soro de queijo, visando à produção de concentrado protéico com baixo teor de fenilalanina
Enzymatic hydrolyzed proteins present some advantages over the pool of amino acids obtained after a chemical hydrolysis: lower osmolality (easier absorption by the organism) and better sensorial characteristics. The removal of phenylalanine (Phe) from these hydrolysates allows their use in the diet of phenylktonuria (PKU) patients. This Thesis studies the se
Publicado em: 2007
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5. Propriedades imunestimulatorias e antitumoral de concentrados proteicos de soro de leite bovino, de caseina e de um isolado proteico de soja. / Antitumoral and imunostimulating properties of bovine milk whey proteics concentrates, of casein and a soy protein isolated.
The objective of the present work was the investigation of immunomodulation and anticancer properties presented by bovine milk proteins with special attention to the whey proteins, in the form of whey protein concentrate (WPC). For such a task, adequate methodology was established for the production of concentrates of caseins and whey proteins, which permitt
Publicado em: 2004
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6. Propriedades fisico-quimicas, nutritivas e funcionais da caseina de leite bovino obtida por diferentes processos
The bovine milk casein concentrates utilized in this work were obtained by different process: enzymatically coagulates casein (COC), casein micelles obtained by microfiltration/diafiltration (CM), and casein micelles obtained from reconstitutes powered milk (CMR). These preparations were compared to a commercial casein (CC) as to physicochemical characterist
Publicado em: 2002
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7. A influencia de ions calcio nas caracteristicas de solubilidade, textura e capacidade de retenção de agua de geis de concentrados e isolado proteicos de soro de leite
Whey protein concentrates (80% protein) and whey protein isolate (90% protein) manufactured by ultrafiltration and diafiltration were evaluated for their solubility properties, textural characteristics and electrophoretic pattem. Concentrates and isolate tested in this study showed high solubility at pHs 7,0 and 4,6 (solubility higher than 84%). Protein solu
Publicado em: 2002
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8. Production of inulinase: recovery and scale-up / Obtenção de inulinase: recuperação e ampliação de escala
It has been observed that the production of extracellular inulinase by Candida kefyr DSM 70106 was markedly affected by the rate of oxygen transfer to the medium. The highest inulinase activity (37,5 U.Ml-1) was attained at a KLa value (volumetric coefficient of oxygen transfer) equal to 43 h-1. As such, KLa was chosen as a criterion for scaling-up the biore
Publicado em: 1995
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9. Reversibility of Protein Binding of Penicillins: an In Vitro Study Employing a Rapid Diafiltration Process
The reversibility of human serum protein binding of oxacillin, nafcillin, and cephalothin was investigated by using a diafiltration technique. While the temperature, pH, and protein concentration were kept constant, 20% of the serum volume was exchanged every 4 to 5 min. The binding of all three drugs was rapidly reversible, and the percentage of protein bin
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10. Improved Method for the Isolation of Biosurfactant Glycolipids from Rhodococcus sp. Strain H13A
An improved method for the isolation of the biosurfactant glycolipids from Rhodococcus sp. strain H13A by using XM 50 diafiltration and isopropanol precipitation was devised. This procedure was advantageous since it removes protein coisolated when the glycolipids are obtained by organic extraction and silicic acid chromatography. The protein apparently does
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11. Reconstitution of D-glucose transport catalyzed by a protein fraction from human erythrocytes in sonicated liposomes.
A protein fraction was obtained from human erythrocyte ghosts by solubilization with Triton X-100 or octylglucoside. Triton X-100 was removed from the protein by Bio-Beads SM-2 and octylglucoside, by diafiltration. The solubilized protein fraction catalyzed D-glucose uptake when reconstituted in sonicated liposomes. The uptake was time dependent and inhibite
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12. Physicochemical Fractionation of Extracellular Cornea-Damaging Proteases of Pseudomonas aeruginosa
Fractionation of the culture supernatant fluids of a cornea-virulent strain of Pseudomonas aeruginosa by ammonium sulfate precipitation, diafiltration, isoelectric focusing, ion-exchange chromatography, gel filtration, and sucrose density gradient centrifugation failed to separate the rabbit cornea-damaging activity and the in vitro protease activity of the