Denaturing Gradient Gel By Electrophoresis
Mostrando 1-12 de 325 artigos, teses e dissertações.
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1. Free-living diazotrophs drive castor bean nitrogen input in tropical semiarid soils
RESUMO: Existem poucos estudos sobre a diversidade microbiana em solos de mamona no ambiente semiárido tropical. Os produtos de mamona têm sido amplamente utilizados em todo o mundo e justificam a importância comercial da ricinocultura no semiárido nordestino brasileiro. Como não há qualquer tipo de fertilização, ou manutenção de resíduos da cult
Cienc. Rural. Publicado em: 02/12/2019
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2. Analysis of microbial diversity in Shenqu with different fermentation times by PCR-DGGE
Abstract Shenqu is a fermented product that is widely used in traditional Chinese medicine (TCM) to treat indigestion; however, the microbial strains in the fermentation process are still unknown. The aim of this study was to investigate microbial diversity in Shenqu using different fermentation time periods. DGGE (polymerase chain reaction-denaturing gradie
Braz. J. Microbiol.. Publicado em: 2017-06
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3. Detection and differentiation of Entamoeba histolytica and Entamoeba dispar in clinical samples through PCR-denaturing gradient gel electrophoresis
Amebiasis is one of the twenty major causes of disease in Mexico; however, the diagnosis is difficult due to limitations of conventional microscopy-based techniques. In this study, we analyzed stool samples using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) to differentiate between Entamoeba histolytica (pathogenic) and E. dis
Braz J Med Biol Res. Publicado em: 03/04/2017
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4. The effect of heavy metal contamination on the bacterial community structure at Jiaozhou Bay, China
Abstract In this study, determination of heavy metal parameters and microbiological characterization of marine sediments obtained from two heavily polluted sites and one low-grade contaminated reference station at Jiaozhou Bay in China were carried out. The microbial communities found in the sampled marine sediments were studied using PCR-DGGE (denaturing gr
Braz. J. Microbiol.. Publicado em: 2017-03
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5. Bacterial Diversity in the Digestive Tracts of Four Indian Air-Breathing Fish Species Investigated by PCR Based Denaturing Gradient Gel Electrophoresis
ABSTRACT An investigation was conducted to identify the allochthonous microbiota (entire intestine) and the autochthonous microbiota in proximal intestine (PI) and distal intestine (DI) of four species of Indian air-breathing fish (climbing perch; Anabas testudineus, murrel; Channa punctatus, walking catfish; Clarias batrachus and stinging catfish; Heteropne
Braz. arch. biol. technol.. Publicado em: 02/12/2016
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6. Evaluation of bacterial diversity recovered from petroleum samples using different physical matrices
ABSTRACT Unraveling the microbial diversity and its complexity in petroleum reservoir environments has been a challenge throughout the years. Despite the techniques developed in order to improve methodologies involving DNA extraction from crude oil, microbial enrichments using different culture conditions can be applied as a way to increase the recovery of D
Braz. J. Microbiol.. Publicado em: 2016-09
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7. Sugarcane trash levels in soil affects the fungi but not bacteria in a short-term field experiment
Abstract The sugarcane in Brazil is passing through a management transition that is leading to the abolition of pre-harvest burning. Without burning, large amounts of sugarcane trash is generated, and there is a discussion regarding the utilization of this biomass in the industry versus keeping it in the field to improve soil quality. To study the effects of
Braz. J. Microbiol.. Publicado em: 2016-06
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8. Bacaba beverage produced by Umutina Brazilian Amerindians: Microbiological and chemical characterization
Bacaba chicha is a beverage prepared by the indigenous Umutina people from the bacaba fruit (Oenocarpus bacaba), a purple berry that is rich in fat and carbohydrates, as well as a source of phenolic compounds. In this study, samples of bacaba chicha beverage were collected, and the microbial community was assessed using culture-dependent and -independent tec
Braz. J. Microbiol.. Publicado em: 27/10/2015
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9. Evaluation of the microbial diversity in sequencing batch reactor treating linear alkylbenzene sulfonate under denitrifying and mesophilic conditions using swine sludge as inoculum
The objective of this study was to evaluate the degradation of Linear Alkylbenzene Sulfonate (LAS) in anaerobic sequencing batch reactor (ASBR) under denitrifying conditions using swine sludge as inoculum. The reactor was operated for 104 days with synthetic substrate containing nitrate, and LAS was added later (22 mg/L). Considering the added mass of the LA
Braz. arch. biol. technol.. Publicado em: 06/03/2015
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10. Microbial dynamics during azo dye degradation in a UASB reactor supplied with yeast extract
The present work aimed to investigate the microbial dynamics during the anaerobic treatment of the azo dye blue HRFL in bench scale upflow anaerobic sludge bed (UASB) reactor operated at ambient temperature. Sludge samples were collected under distinct operational phases, when the reactor were stable (low variation of color removal), to assess the effect of
Braz. J. Microbiol.. Publicado em: 2014-12
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11. Spectrum of K ras mutations in Pakistani colorectal cancer patients
The incidence of colorectal cancer (CRC) is increasing daily worldwide. Although different aspects of CRC have been studied in other parts of the world, relatively little or almost no information is available in Pakistan about different aspects of this disease at the molecular level. The present study was aimed at determining the frequency and prevalence of
Braz J Med Biol Res. Publicado em: 29/11/2013
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12. Characterization of spoilage bacteria in pork sausage by PCR-DGGE analysis
To investigate microbial diversity and identify spoilage bacteria in fresh pork sausages during storage, twelve industrial pork sausages of different trademarks were stored at 4 ºC for 0, 14, 28 and 42 days, 80% relative humidity and packaged in sterile plastic bags. Microbiological analysis was performed. The pH and water activity (a w) were measured. The
Food Sci. Technol. Publicado em: 24/09/2013