Ddrt Pcr
Mostrando 13-24 de 26 artigos, teses e dissertações.
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13. Expressão genica diferencial em reticulocitos de pacientes com doença da hemoglobina H / Differential gene expression in reticulocytes of Hemoglobin H disease patients
Hb H disease results from the inactivation of three of the four alfa -globin genes normally present on diploid genome and it is characterized by a moderate to severe chronic hemolytic anemia. Patients usually present microcytosis, hypochromia and poikilocytosis, with 25 to 30% of Hb Bart?s (?4) at birth and 5 to 30% of Hb H (?4) in adult life. Although the m
Publicado em: 2007
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14. Differentially displayed expressed sequence tags in Melipona scutellaris (Hymenoptera, Apidae, Meliponini) development
Nesse estudo nós usamos a técnica de Differential Display Reverse Transcriptase - Polymerase Chain Reaction (DDRT-PCR) para comparamos o perfil de mRNA em Melipona scutellaris durante o desenvolvimento ontogenético pós-embrionário e em operárias adultas, rainha natural e induzida pelo Hormônio Juvenil III. Fragmentos diferencialmente expressos foram d
Anais da Academia Brasileira de Ciências. Publicado em: 2006-03
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15. DetecÃÃo de genes diferencialmente expressos na formiga urbana Camponotus vittatus (Hymenoptera, Formicinae)
Social insects inspire great curiosity, interest and a wish to know the social life molecular basis. Differentially expressed genes identification has been used with the objective to understand either the gene function and the molecular mechanisms of different biological processes. In this study, two protocols for the total RNA extraction from Camponotus vit
Publicado em: 2006
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16. Detecção de genes diferencialmente expressos na formiga urbana Camponotus vittatus (Hymenoptera, Formicinae)
Insetos sociais despertam grande curiosidade, interesse e uma vontade de se compreender as bases moleculares da vida social. A identificação de genes diferencialmente expressos tem sido usada para o entendimento não só da função gênica, mas também dos mecanismos moleculares de diferentes processos biológicos. Nesse estudo, foram testados dois protoc
Publicado em: 2006
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17. Identificação de transcritos de Ceriporiopsis subvermispora expressos nas fases iniciais da biodegradação da madeira / Identification of transcripts of Ceriporiosis subvermispora expressed during initial phases of wood biodegradation
Lignin is a heterogeneous and highly cross-linked macromolecule that is particularly resistant to biological degradation but some specialized fungi developed the ability to degrade this complex molecule. Most of these fungi belong to the basidiomycetes and are responsible for the white-rot decay processo One of them, Ceriporiopsis subvermispora, has been ext
Publicado em: 2006
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18. Identification of differentially expressed genes in queen and worker castes of Melipona quadrifasciata / Identificação de genes diferencialmente expressos nas castas da rainha e operaria de Melipona quadrifasciata
Caste polyphenism is a multifaceted phenomenon, most evident in the marked differences in reproductive capacity and longevity between queens and workers. In the present study, the stingless bee Melipona quadrifasciata anthidioides was chosen to investigate differences in gene expression between newly emerged adult queens and workers. This study has focused o
Publicado em: 2005
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19. Identificação de marcadores moleculares para o câncer de tireóide por cDNA microarrays / Identification of molecular markers for thyroid cancer by cDNA microarrays
Doenças tireoideanas são bastante comuns, sendo sua maioria benigna. A relação entre os diversos tipos de doenças tireoideanas, bem como seus aspectos moleculares, são pouco conhecidos. O bócio (hiperplasia), por exemplo, é descrito por alguns como relacionado com carcinoma (tumor maligno) papilífero, enquanto que outros afirmam não haver relação
Publicado em: 2003
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20. Applications of Differential-Display Reverse Transcription-PCR to Molecular Pathogenesis and Medical Mycology
The host-fungus interaction is characterized by changes in gene expression in both host and pathogen. Differential-display reverse transcription PCR (DDRT-PCR) is a PCR-based method that allows extensive analysis of gene expression among several cell populations. Several limitations and drawbacks to this procedure have now been addressed, including the large
American Society for Microbiology.
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21. Efficient reamplification of differential display products by transient ligation and thermal asymmetric PCR.
A new method for specific reamplification of DDRT-PCR products is presented. After transient ligation of the primary DDRT-PCR fragments into a T-vector, the cDNAs of interest were reamplified by hemi-nested PCR and thermally asymmetric cycles. In contrast to the originally described protocol, this method of reamplification is specific, sensitive, reproducibl
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22. Identification of differentially expressed mRNA species by an improved display technique (DDRT-PCR).
We have significantly improved a method originally developed by Liang and Pardee [Science 257 (1992) 967-971] to display a broad spectrum of expressed genes and to detect differences in expression between different cell types. We have analysed various aspects of the technique and have modified it for both, the application to fast and efficient identification
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23. Expression of a novel mRNA in human head and neck squamous cell carcinoma cells.
AIMS: The differential display reverse transcription polymerase chain reaction (DDRT-PCR) technique was used to search for differences between the mRNA expression profiles of squamous cell carcinoma (SCC) cell lines established from head and neck tumours and normal keratinocytes from the mucosa of the upper aerodigestive tract. METHODS: Total RNA prepared fr
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24. Identification of differentially expressed mRNAs in human fetal liver across gestation.
Differential gene expression, with its precise start and stop times, is believed to be critical for the programmed development of new cells and tissues. Within the developing fetus, one tissue of particular interest is fetal liver. This organ undergoes rapid changes in the pathway toward liver development in utero since it is also the major site of hematopoi