D Fructofuranosidase
Mostrando 1-11 de 11 artigos, teses e dissertações.
-
1. Double mutation of Saccharomyces cerevisiae for enhanced β-D-fructofuranosidase fructohydrolase productivity and application of growth kinetics for parametric significance analysis
Abstract The kinetics of an extracellular β-D-fructofuranosidase fructohydrolase production by Saccharomyces cerevisiae in a chemically defined medium, i.e., sucrose peptone agar yeast extract at pH 6, was investigated. The wild-type was treated with a chemical mutagen, methyl methane sulfonate. Among the six mutants isolated, methyl methane sulfonate-V was
Braz. J. Microbiol.. Publicado em: 2016-03
-
2. Produção de invertases termoestáveis por Aspergillus caespitosus em fermentação submersa e em estado sólido usando resíduos agroindustriais como fonte de carbono
O fungo filamentoso Aspergillus caespitosus foi um bom produtor de invertases intracelular e extracelular em fermentação submersa (FSbm) ou em estado sólido (FES), usando resíduos agroindustriais como fonte de carbono, sendo que para ambas as condições de cultivo, a maior produtividade foi obtida empregandose farelo de trigo. A produção da forma extr
Brazilian Journal of Microbiology. Publicado em: 2009-09
-
3. ImobilizaÃÃo covalente de Ã-Dfrutofuranosidase (invertase) em suporte vÃtreo-cerÃmico
Ã-D-Fructofuranosidase (invertase, E.C. 3.2.1.26) of Saccharomyces cerevisiae was covalently immobilized at ceramic support surface synthesized from fly ashes of mineral coal added of glass beads (50-100μm), modified with ?- aminopropiltriethylxisilane (APTS), followed by glutaraldehyde which acts as agent bifuncional responsible for the cross-linked a
Publicado em: 2004
-
4. Estudo do Processo Continuo para ProduÃÃo de AÃucar invertido por via EnzimÃtica / Study of the Process I continue for Production of inverted sugar for saw Enzymatic
A enzima invertase (E.C.3.2.1.26-D-fructofuranosidase) catalisa a hidrÃlise da sacarose produzindo uma mistura equimolar de glicose e frutose denominada aÃÃcar invertido, que possui maior poder edulcorante, Ã mais facilmente adicionada em preparos industriais e possui maior valor agregado que a sacarose. O fungo Cladosporium cladosporioides possui invert
Publicado em: 2003
-
5. Neurospora Mutant Exhibiting Hyperproduction of Amylase and Invertase
A mutant strain of Neurospora crassa has been isolated which is derepressed for amylase and β-fructofuranosidase (invertase). Large amounts of the two enzymes were secreted into the culture medium upon depletion of exogenous carbon source. The resulting increases of the two extracellular enzymes were prevented by actinomycin D, cycloheximide, and glycerol.
-
6. Characterization of Invertase Activity from Cariogenic Streptococcus mutans
Invertase activity from Streptococcus mutans GS-5 has been partially purified and shown to possess β-fructofuranosidase specificity. The enzyme has a broad pH optimum between pH 5.5 and 7.5 and exhibits maximal activity at 37 C. Fructose, but not the glucose analogue α-methyl-d-glucoside, acts as a competitive inhibitor of the enzyme. None of the common gl
-
7. Purification and characterization of neutral and alkaline invertase from carrot.
Neutral and alkaline invertase were identified in cells of a suspension culture of carrot (Daucus carota L.) and purified to electrophoretic homogeneity. Neutral invertase is an octamer with a molecular mass of 456 kD and subunits of 57 kD, whereas alkaline invertase is a tetramer with a molecular mass of 504 kD and subunits of 126 kD. Both enzymes had sharp
-
8. Purification and characterization of three soluble invertases from barley (Hordeum vulgare L.) leaves.
Three soluble isoforms of invertase (beta-fructofuranosidase; EC 3.2.1.26) were purified from 7-d-old primary leaves of barley (Hordeum vulgare L.). Invertase I, a monomeric protein of 64 kD, was purified to apparent homogeneity as shown by sodium dodecylsulfate-polyacrylamide gel electrophoresis. Invertases IIA and IIB, multimeric proteins with molecular ma
-
9. Sucrolytic Enzyme Activities in Cotyledons of the Faba Bean (Developmental Changes and Purification of Alkaline Invertase).
In terms of maximum extractable catalytic activity, sucrose synthase is the predominant sucrolytic enzyme in developing cotyledons of faba bean (Vicia faba L.). Although acid invertase activity is extremely low, there is significant activity of alkaline invertase, the majority of which is extractable only with high concentrations of NaCl. Calculations of pot
-
10. Degradation of Sucrose by Whole Cells and Plaque of Actinomyces naeslundii
Whole cells of Actinomyces naeslundii ATCC 12104, either in a dispersed form or in the form of plaque, enzymatically degraded sucrose to glucose and fructose. Washed whole cells expressed β-fructofuranosidase specificity and hydrolyzed sucrose to essentially equimolar quantities of glucose and fructose. The cells readily hydrolyzed sucrose, raffinose, and A
-
11. Molecular characterization of a fructanase produced by Bacteroides fragilis BF-1.
The Bacteroides fragilis BF-1 fructanase-encoding gene (fruA) was cloned and expressed in Escherichia coli from the recombinant plasmid pBS100. The fruA gene consisted of 1,866 bp encoding a protein of 622 amino acids with a calculated M(r) of 70,286. The apparent M(r) of the fructanase, determined by in vitro cell-free transcription-translation and sodium d