Cysteine Protease Inhibitor
Mostrando 1-12 de 109 artigos, teses e dissertações.
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1. OTIMIZAÇÃO E VALIDAÇÃO DE MÉTODO ENZIMÁTICO SEMIQUANTITATIVO SIMPLES E DE BAIXO CUSTO PARA A BUSCA DE INIBIDORES DE CISTEINO PROTEASES
Cysteine proteases are an important class of enzymes involved in several essential biological processes. For this reason, these enzymes are important therapeutic targets, and therefore can be used in enzymatic assays for the search of hits. The most widely used methods in the search of enzyme inhibitors are spectrophotometric, however they have relatively hi
Quím. Nova. Publicado em: 2021-04
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2. New Synthetic Quinolines as Cathepsin K Inhibitors
Cathepsin K is a papain-like cysteine protease and is responsible for collagen degradation in bone tissue and thus represents an important target for the development of new therapies for treating diseases such as osteoporosis. Quinolines are an important class of heterocyclic molecular leads with a great pharmacological potential and represent a relevant sca
J. Braz. Chem. Soc.. Publicado em: 2020-08
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3. Odanacatib Inhibits Resistin-induced Hypertrophic H9c2 Cardiomyoblast Cells Through LKB1/AMPK Pathway
ABSTRACT Odanacatib (ODN) is a selective inhibitor of cathepsin K. The cysteine protease cathepsin K has been implicated in cardiac hypertrophy. Resistine is an adipokine which is identified to promote cardiac hypertrophy. Here, we hypothesize that ODN mitigates resistin-induced myocyte hypertrophy. Cell surface area and protein synthesis were measured after
Braz. arch. biol. technol.. Publicado em: 17/08/2017
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4. Transcriptome analysis of resistant soybean roots infected by Meloidogyne javanica
Soybean is an important crop for Brazilian agribusiness. However, many factors can limit its production, especially root-knot nematode infection. Studies on the mechanisms employed by the resistant soybean genotypes to prevent infection by these nematodes are of great interest for breeders. For these reasons, the aim of this work is to characterize the trans
Genet. Mol. Biol.. Publicado em: 2012
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5. Theobroma cacao cystatins impair Moniliophthora perniciosa mycelial growth and are involved in postponing cell death symptoms.
Three cystatin open reading frames named TcCys1, TcCys2 and TcCys3 were identified in cDNA libraries from compatible interactions between Theobroma cacao (cacao) and Moniliophthora perniciosa. In addition, an ORF named TcCys4 was identified in the cDNA library of the incompatible interaction. The cDNAs encoded conceptual proteins with 209, 127, 124, and 205
Planta. Publicado em: 2011
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6. Partial purification and characterization of serine and cysteine proteases of Sitophilus zeamais (Coleoptera: Curculionidae) / Purificação e caracterização parciais de serino e cisteíno proteases de Sitophilus zeamais (Coleoptera: Curculionidae)
Serine and cysteine proteases of a susceptible and two resistant strain (one with a cost associated with resistance and other without cost) of the maize weevil Sitophilus zeamais were partially purified using an affinity column of aprotinin-agarose . The highest purification factor was obtained for the resistant no-cost strain, 51.20x, with a yield of 14.8%
Publicado em: 2009
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7. Propriedades bioquímicas e cinético-enzimáticas de cisteíno- proteases do intestino médio da lagarta da soja / Biochemistry and kinectics-enzymatics properties of cysteine-proteases of the velvetbean caterpillar midgut
Protease inhibitors that react with insect specific proteases are candidates to have their genetic code inserted in genetically modified plants. The first step to achieve this is the characterization of proteolytic enzymes of these insects intestines. Cysteine-protease of the soluble and insoluble fractions from the midgut of Anticarsia gemmatalis were chara
Publicado em: 2008
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8. Expressão recombinante da canacistatina em células de inseto.
Due to the great economic importance of sugarcane crop in Brazil, the occurrence of infections by phytopathogens leading to decreased productivity and quality remains a serious problem. As the plants have natural mechanisms of defense against the attack of fungi and insects, amongst which the inhibitors of proteases are distinguished, the use of these substa
Publicado em: 2007
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9. Isolamento e caracterização de um novo conjunto de serinoproteases com atividade trombina-like e de L-aminoacido oxidase do veneno de Crotalus durissus cascavella / Isolation and characterization of a new serine proteases group with thrombinin-like and L-amino acid oxidase activity from Crotalus durissus cascavella
The use of the isolated toxins from poisons as molecular tools in the understanding of diverse physiological and pathological events has been proved by some works in literature. The serpent Crotalus durissus cascavella is found in the areas of Caatinga Northeast of Brazil, since Maranhão until North of the state of Minas Gerais, and in spite of it hasn?t be
Publicado em: 2006
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10. Bmcystatin, cysteine protease inhibitor present in fat body of Boophilus microplus tick: Cloning, expression, purification and characterization. / BmCistatina, Inibidor de cisteinoproteases presente em corpo gorduroso de carrapato Boophilus microplus: Clonagem, expressão, purificação e caracterização.
A importância econômica dos carrapatos está relacionada a seus hábitos hematofágicos. Entre as espécies que pertencem à família Ixodidae, o carrapato Boophilus microplus tem grande importância veterinária por ser vetor de patógenos, atuando como ectoparasito em animais domésticos e silvestres, transmitindo a doença conhecida como o babesiosis e
Publicado em: 2006
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11. Endogenous inhibitor for calcium-dependent cysteine protease contains four internal repeats that could be responsible for its multiple reactive sites.
A cDNA encoding an endogenous inhibitor, termed calpastatin, for calcium-dependent cysteine protease (calpain, EC 3.4.22.17) was cloned by screening rabbit cDNA libraries with a synthetic oligodeoxynucleotide probe based on the partial amino acid sequence of the purified protein. The deduced amino acid sequence contains 718 amino acid residues (Mr, 76,964),
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12. The involvement of cysteine proteases and protease inhibitor genes in the regulation of programmed cell death in plants.
Programmed cell death (PCD) is a process by which cells in many organisms die. The basic morphological and biochemical features of PCD are conserved between the animal and plant kingdoms. Cysteine proteases have emerged as key enzymes in the regulation of animal PCD. Here, we show that in soybean cells, PCD-activating oxidative stress induced a set of cystei