Cotransformation
Mostrando 1-12 de 106 artigos, teses e dissertações.
-
1. Plant tissue culture and regeneration of transgenic plants from embryogenic callus and immature leaves of sugarcane / Cultura de tecidos e regeneração de plantas transgênicas a partir de calos embriogênicos e de folhas imaturas de cana-de-açúcar
Sugarcane is a polyploidy monocot and allogamous species that has low reproductive rate due to the difficulty of flowering. Because of these genetic and physiological characteristics breeding program takes long time and demand hard labor. Alternatively, modern biotechnology approaches contribute to the development of new cultivars. In this work we studied th
Publicado em: 2010
-
2. Genetic transformation of sugarcane by biolistic and Agrobacterium tumefaciens to study the mechanism of programmed cell death / Transformação genética de cana-de-açúcar por biolística e Agrobacterium tumefaciens visando estudar o mecanismo de morte celular programada
Sugarcane is one of the main crops planted in Brazil and presents significant socioeconomic and agribusiness importance to the country. The world scene is quite favorable as regards the marketing of its two main products, sugar and alcohol, driving the development of the national sugar-alcohol sector. Therefore, the sugarcane genetic breeding appears as the
Publicado em: 2009
-
3. Resistance to Anticarsia gemmatalis Hübner (Lepidoptera, Noctuidae) in transgenic soybean (Glycine max (L.) Merrill Fabales, Fabaceae) cultivar IAS5 expressing a modified Cry1Ac endotoxin
Somatic embryos of the commercial soybean (Glycine max) cultivar IAS5 were co-transformed using particle bombardment with a synthetic form of the Bacillus thuringiensis delta-endotoxin crystal protein gene cry1Ac, the beta-glucuronidase reporter gene gusA and the hygromycin resistance gene hpt. Hygromycin-resistant tissues were proliferated individually to g
Genetics and Molecular Biology. Publicado em: 2008
-
4. Obtenção e caracterização de linhagens recombinantes de Penicillium griseoroseum com alta produção de pectina liase e poligalacturonase / Obtainment and characterizing of recombinant strains of Penicillium griseoroseum with pectin lyase and polygalacturonase overproduction
Penicillium griseoroseum has been described as a promising species for polygalacturonase (PG) and pectin lyase (PL) production. However, the genes encoding these enzymes in this organism require induction by pectin and are repressed by glucose. One strategy to increase the expression of these genes is the replacement of original promoter by a strong constitu
Publicado em: 2007
-
5. Co-transformation of a tropical maize endophytic isolate of Fusarium verticillioides (synonym F. moniliforme) with gusA and nia genes
A tropical endophytic isolate of the fungus Fusarium verticillioides (synonym Fusarium moniliforme) obtained from Zea mays was co-transformed with plasmid pNH24 containing the Fusarium oxysporum nitrate reductase nia gene and plasmid pNOM 102 carrying the Escherichia coli b-glucuronidase gusA gene. Transformation frequency for the nia marker was 75 transform
Genetics and Molecular Biology. Publicado em: 2004
-
6. Efficient nuclear localization and immortalizing ability, two functions dependent on the adenovirus type 5 (Ad5) E1A second exon, are necessary for cotransformation with Ad5 E1B but not with T24ras.
Expression of adenovirus type 5 E1A 12S is sufficient to immortalize primary baby rat kidney cells, but another viral or cellular oncogene, such as E1B or T24ras, is necessary for complete transformation. The regions of 12S sufficient for T24ras cotransformation have been well characterized and are located in the first exon. The second exon is dispensable fo
-
7. Cotransformation of a serum resistance phenotype with genes for arginine biosynthesis in Neisseria gonorrhoeae.
Two genes (arg-3 and arp-6) marked by arginine auxotrophy and a gene (sac-2) marked by resistance to serum killing were found to be linked by cotransformation. Evidence derived from the linkage of these markers to spc (spectinomycin resistance) suggests that sac-2 may be genetically distinct from a sac gene previously described.
-
8. Cooperative transformation studies with temperature-sensitive mutants of Rous sarcoma virus.
Stocks of Rous sarcoma virus Bryan strain were mutagenized using a bromodeoxyuridine treatment immediately after infection. Thirty temperature-sensitive (ts) mutants defective in transformation (td) were isolated by a replica plating technique. Twenty of these mutants were preliminarily characterized and found to be defective in late functions related to tra
-
9. Cotransformation and gene targeting in mouse embryonic stem cells.
We have investigated cotransformation in mammalian cells and its potential for identifying cells that have been modified by gene targeting. Selectable genes on separate DNA fragments were simultaneously introduced into cells by coelectroporation. When the introduced fragments were scored for random integration, 75% of the transformed cells integrated both fr
-
10. Cotransformation and Targeted Gene Inactivation in the Maize Anthracnose Fungus, Glomerella graminicola†
Cotransformation of Glomerella graminicola was achieved with the G. graminicola genes TUB1R1 (encoding a β-tubulin which confers resistance to the fungicide benomyl) and PYR1 (encoding orotate phosphoribosyl transferase, which confers pyrimidine prototrophy). The cotransformation frequency was about 30% when selection was for pyrimidine prototrophy (Pyr+) a
-
11. Congression of Unlinked Markers and Genetic Mapping in the Transformation of BACILLUS SUBTILIS 168
A thorough examination of cotransformation of two unlinked genetic markers in Bacillus subtilis 168 shows that the two recombinational events do not occur randomly. The cotransformation frequency is dependent on the distance between the two markers as well as on the order in which they replicate in the competent cell. These results indicate that uptake and/o
-
12. Homologous recombination and the repair of double-strand breaks during cotransformation of Dictyostelium discoideum.
We examined the ability of unlinked nonreplicating plasmid molecules to undergo homologous recombination during cotransformation of Dictyostelium amoebae. The transformation vector B10S confers resistance to the antibiotic G418 and was always presented to amoebae as a closed circle. Cotransforming DNA, containing a slime mold cDNA and sequences homologous to