Conventional Multiplex Pcr
Mostrando 1-12 de 60 artigos, teses e dissertações.
-
1. Evaluation of a rapid detection method of Salmonella in comparison with the culture method and microbiological quality in fish from the Brazilian Amazon
Abstract Microbiological safety of fish is a concern of consumers, industries and regulatory agencies worldwide. Among the pathogenic microorganisms, Salmonella spp. is one of the main agents of foodborne diseases and should be absent in animal products. Rapid and accurate identification of pathogens in the supply chain is important for both quality assuranc
Food Sci. Technol. Publicado em: 2021-03
-
2. Field performance of hermaphrodite papaya plants obtained through molecular selection and micropropagation
Abstract Most commercial papaya varieties segregate hermaphrodite and female plants. Growers normally select hermaphrodite plants in the field, due to market preferences. This requires planting multiple plants per site and later thinning of the females, distinguished by flower bud inspection. Micropropagation or the use of molecular markers are two possibili
Crop Breed. Appl. Biotechnol.. Publicado em: 05/12/2019
-
3. Multiplex TaqMan qPCR assay for specific identification of encapsulated Trichinella species prevalent in North America
BACKGROUND Human trichinellosis is a foodborne parasitic zoonotic disease caused by ingestion of raw or undercooked meat infected with nematode larvae of the genus Trichinella. In the USA, sporadic cases and outbreaks caused by the consumption of wild game meat infected with Trichinella have been reported. The current methods for diagnosis such as serology a
Mem. Inst. Oswaldo Cruz. Publicado em: 29/10/2018
-
4. PCR-RFLP assay as an option for primary HPV test
Persistent human papillomavirus (HPV) infection is an essential factor of cervical cancer. This study evaluated the analytical performance of restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP) assay compared to PapilloCheck® microarray to identify human papilloma virus (HPV) in cervical cells. Three hundred and twenty-five women w
Braz J Med Biol Res. Publicado em: 26/03/2018
-
5. Prevalence and serotype distribution of Listeria monocytogenes isolated from foods in Montevideo-Uruguay
ABSTRACT The aim of this work was to study the prevalence of Listeria monocytogenes in foods obtained in retail shops and food industries located in Montevideo-Uruguay, and to identify the serogroups of the obtained isolates. Three-thousand one-hundred and seventy-five food samples (frozen, deli meats, ready-to-eat and cheese) were analyzed. The obtained iso
Braz. J. Microbiol.. Publicado em: 2017-12
-
6. A novel multiplex PCR for the simultaneous detection of Salmonella enterica and Shigella species
Salmonella enterica and Shigella species are commonly associated with food and water borne infections leading to gastrointestinal diseases. The present work was undertaken to develop a sensitive and reliable PCR based detection system for simultaneous detection of Salmonella enterica and Shigella at species level. For this the conserved regions of specific g
Braz. J. Microbiol.. Publicado em: 25/07/2014
-
7. The performance of four molecular methods for the laboratory diagnosis of congenital toxoplasmosis in amniotic fluid samples
Introduction Toxoplasmosis may be life-threatening in fetuses and in immune-deficient patients. Conventional laboratory diagnosis of toxoplasmosis is based on the presence of IgM and IgG anti-Toxoplasma gondii antibodies; however, molecular techniques have emerged as alternative tools due to their increased sensitivity. The aim of this study was to compare
Rev. Soc. Bras. Med. Trop.. Publicado em: 15/10/2013
-
8. Rapid detection and differentiation of mycobacterial species using a multiplex PCR system
Introduction The early diagnosis of mycobacterial infections is a critical step for initiating treatment and curing the patient. Molecular analytical methods have led to considerable improvements in the speed and accuracy of mycobacteria detection. Methods The purpose of this study was to evaluate a multiplex polymerase chain reaction system using mycobac
Rev. Soc. Bras. Med. Trop.. Publicado em: 2013-07
-
9. Multiplex polymerase chain reaction to identify and determine the toxigenicity of Corynebacterium spp with zoonotic potential and an overview of human and animal infections
Corynebacterium diphtheriae, Corynebacterium ulcerans and Corynebacterium pseudotuberculosis constitute a group of potentially toxigenic microorganisms that are related to different infectious processes in animal and human hosts. Currently, there is a lack of information on the prevalence of disease caused by these pathogens, which is partially due to a red
Mem. Inst. Oswaldo Cruz. Publicado em: 2013-05
-
10. Incidence and multiplex PCR based detection of trichothecene chemotypes of Fusarium culmorum isolates collected from freshly harvested Maize kernels in Southern India
Hundred Fusarium culmorum strains, isolated from freshly harvested maize grain samples from Southern parts of India, were incubated in czapek-dox medium and analyzed for trichothecene (DON/NIV) production. The mPCR assay was standardized targeting trichothecene metabolic pathway genes viz., Tri6, Tri7, Tri13 for detection of trichothecene (DON/NIV) chemotype
Braz. J. Microbiol.. Publicado em: 2013
-
11. Application of the mammalian glyceraldehyde-3-phosphate dehydrogenase gene for sample quality control in multiplex PCR for diagnosis of leishmaniasis
Leishmaniasis is a neglected disease endemic in five continents. It is a severe disease that may lead to death, and its early detection is important to avoid severe damage to affected individuals. Molecular methods to detect Leishmania are considered alternatives to overcome the limitations presented by conventional methods. The aim of this study was to deve
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2012
-
12. Avaliação microbiana das manchas dentárias extrínsecas negras em pacientes submetidos a tratamento ortodôntico / Bacterial analysis of black tooth stains in patients on orthodontic treatment
Black extrinsic tooth stains appear on the smooth surfaces of teeth, parallel to the marginal gum and are considered a specific form of biofilm due to the high content of calcium, phosphate and insoluble iron salt. This study evaluated the profile of bacterial biofilms in patients with black extrinsic tooth stain with or without fixed metal braces installed.
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 04/07/2011