Complementary Dna
Mostrando 13-24 de 2644 artigos, teses e dissertações.
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13. Arachnids of medical importance in Brazil: main active compounds present in scorpion and spider venoms and tick saliva
Arachnida is the largest class among the arthropods, constituting over 60,000 described species (spiders, mites, ticks, scorpions, palpigrades, pseudoscorpions, solpugids and harvestmen). Many accidents are caused by arachnids, especially spiders and scorpions, while some diseases can be transmitted by mites and ticks. These animals are widely dispersed in u
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 29/09/2015
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14. Characterisation of divergent flavivirus NS3 and NS5 protein sequences detected in Rhipicephalus microplus ticks from Brazil
Transcripts similar to those that encode the nonstructural (NS) proteins NS3 and NS5 from flaviviruses were found in a salivary gland (SG) complementary DNA (cDNA) library from the cattle tick Rhipicephalus microplus.Tick extracts were cultured with cells to enable the isolation of viruses capable of replicating in cultured invertebrate and vertebrate cells.
Mem. Inst. Oswaldo Cruz. Publicado em: 10/10/2013
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15. In vitro detection of pathogenic Listeria monocytogenes from food sources by conventional, molecular and cell culture method
Among current in vitro methods for identification of pathogenic Listeria monocytogenes (L. monocytogenes) rely on growth in culture media, followed by isolation, and biochemical and serological identification. Now PCR (Polymerase Chain Reaction) has been used for the rapid, sensitive and specific detection of pathogenic L. monocytogenes. The pathogenicity of
Braz. J. Microbiol.. Publicado em: 2013-09
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16. Genetic architecture of purple pigmentation and tagging of some loci to SSR markers in pearl millet, Pennisetum glaucum (L.) R. Br.
This report describes the construction of integrated genetic maps in pearl millet involving certain purple phenotype and simple sequence repeat (SSR) markers. These maps provide a direct means of implementing DNA marker-assisted selection and of facilitating "map-based cloning" for engineering novel traits. The purple pigmentation of leaf sheath, midrib and
Genetics and Molecular Biology. Publicado em: 16/02/2012
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17. Caracterização de determinantes genéticos envolvidos na qualidade industrial e nutricional do fruto de tomate / Characterization of genetic determinants involved in industrial and nutritional quality of tomato fruit
This thesis addresses tomato fruit metabolism analysis by using three complementary approaches: i) the identification of candidate genes co-located with QTL previously described from metabolic profiles of S. lycopersicum (cv. M82) and S. pennellii introgressed lines, associated with industrial and nutritional quality traits; ii) the structural analyses of th
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 11/08/2011
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18. Differential gene expression, induced by salicylic acid and Fusarium oxysporum f. sp. lycopersici infection, in tomato.
The objective of this work was to determine the transcript profile of tomato plants (Lycopersicon esculentum Mill.), during Fusarium oxysporum f. sp. lycopersici infection and after foliar application of salicylic acid. The suppression subtractive hybridization (SSH) technique was used to generate a cDNA library enriched for transcripts differentially expr
Pesquisa Agropecuária Brasileira. Publicado em: 2011
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19. Exploitation of mitochondrial nad6 as a complementary marker for studying population variability in Lepidoptera
The applicability of mitochondrial nad6 sequences to studies of DNA and population variability in Lepidoptera was tested in four species of economically important moths and one of wild butterflies. The genetic information so obtained was compared to that of cox1 sequences for two species of Lepidoptera. nad6 primers appropriately amplified all the tested DNA
Genetics and Molecular Biology. Publicado em: 2011
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20. Non-homologous DNA end joining in normal and cancer cells and its dependence on break structures
DNA double-strand breaks (DSBs) are a serious threat to the cell, for if not or miss-repaired, they can lead to chromosomal aberration, mutation and cancer. DSBs in human cells are repaired via non-homologous DNA end joining (NHEJ) and homologous recombination repair pathways. In the former process, the structure of DNA termini plays an important role, as do
Genetics and Molecular Biology. Publicado em: 12/03/2010
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21. cDNA and deduced primary structure of basic phospholipase A2 with neurotoxic activity from the venom secretion of the Crotalus durissus collilineatus rattlesnake
To illustrate the construction of precursor complementary DNAs, we isolated mRNAs from whole venom samples. After reverse transcription polymerase chain reaction (RT-PCR), we amplified the cDNA coding for a neurotoxic protein, phospholipase A2 D49 (PLA2 D49), from the venom of Crotalus durissus collilineatus (Cdc PLA2). The cDNA encoding Cdc PLA2 from whole
Brazilian Journal of Medical and Biological Research. Publicado em: 2010-03
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22. Genetic signatures of parental contribution in black and white populations in Brazil
Two hundred and three individuals classified as white were tested for 11 single nucleotide polymorphisms plus two insertion/deletions in their Y-chromosomes. A subset of these individuals (n = 172) was also screened for sequences in the first hypervariable segment of their mitochondrial DNA (mtDNA). In addition, complementary studies were done for 11 of the
Publicado em: 2010
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23. Application of broad-range bacterial PCR amplification and direct sequencing on the diagnosis of neonatal sepsis
A broad-range bacterial PCR target to conserved regions of the 23S rDNA was applied to 306 blood culture samples from 295 infants (up to one year of age) admitted to a neonatal intensive care unit. Classic blood culture results were compared to DNA sequencing analysis of the PCR amplification products. Culture results were in agreement to DNA sequencing in 9
Publicado em: 2010
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24. Modelo de sistema de comunicações digital para o mecanismo de importação de proteinas mitocondriais atraves de codigos corretores de erros / Digital communication system model for mitochondrial protein import by use of error-correcting codes
One of the puzzling problems in mathematical biology is to show the existence of any form of error-correcting code in the DNA structure. Using information theory considerations we propose a model for the biological coding system similar to that of a digital communication system. This model consists of a mapper (transformations from the set of nucleotides eit
Publicado em: 2010