Compatible Solutes
Mostrando 13-24 de 62 artigos, teses e dissertações.
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13. Effects of Temperature, Salinity, and Medium Composition on Compatible Solute Accumulation by Thermococcus spp.
The effects of salinity and growth temperature on the accumulation of intracellular organic solutes were examined by nuclear magnetic resonance spectroscopy (NMR) in Thermococcus litoralis, Thermococcus celer, Thermococcus stetteri, and Thermococcus zilligii (strain AN1). In addition, the effects of growth stage and composition of the medium were studied in
American Society for Microbiology.
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14. Protection of Pyruvate,Pi Dikinase from Maize against Cold Lability by Compatible Solutes 1
Most C4 species are chilling sensitive and certain enzymes like pyruvate,Pi dikinase of the C4 pathway are also cold labile. The ability of cations and compatible solutes to protect maize (Zea mays) dikinase against cold lability was examined. The enzyme in desalted extracts at pH 8 from preilluminated leaves could be protected against cold lability (at 0°C
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15. Regulation of Transcription of Compatible Solute Transporters by the General Stress Sigma Factor, σB, in Listeria monocytogenes†
Listeria monocytogenes is well known for its durable physiological characteristics, which allow the organism to grow at low temperature and pH and high osmolarity. Growth under high osmolarity depends on the accumulation of compatible solutes, among which glycine betaine and carnitine are the preferred solutes for this organism. Three different transport sys
American Society for Microbiology.
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16. Compatible-Solute-Supported Periplasmic Expression of Functional Recombinant Proteins under Stress Conditions
The standard method of producing recombinant proteins such as immunotoxins (rITs) in large quantities is to transform gram-negative bacteria and subsequently recover the desired protein from inclusion bodies by intensive de- and renaturing procedures. The major disadvantage of this technique is the low yield of active protein. Here we report the development
American Society for Microbiology.
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17. LeProT1, a transporter for proline, glycine betaine, and gamma-amino butyric acid in tomato pollen.
During maturation, pollen undergoes a period of dehydration accompanied by the accumulation of compatible solutes. Solute import across the pollen plasma membrane, which occurs via proteinaceous transporters, is required to support pollen development and also for subsequent germination and pollen tube growth. Analysis of the free amino acid composition of va
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18. A prominent role for glucosylglycerol in the adaptation of Pseudomonas mendocina SKB70 to osmotic stress.
The mechanism of osmoadaptation in a salt-tolerant (1.2 M NaCl) bacterial isolate identified as Pseudomonas mendocina (N. J. Palleroni, M. Doudoroff, R. Y. Stanier, R. E. Solanes, and R. Mandel, J. Gen. Microbiol. 60:215-231, 1970) was investigated. In response to osmotic stress, this species accumulated a number of compatible solutes, the intracellular leve
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19. Responses of Halophytes to High Salinities and Low Water Potentials 1
The effects of nonsaline polyethylene glycol (PEG)-6000 and saline seawater solutions of comparable osmotic potential on the concentrations of organic solutes and inorganic ions in the tissues of halophytes (Plantago maritima L., Triglochin maritima L., Limonium vulgare Mill., Halimione portulacoides (L.) Aell) have been investigated. Studies were made to de
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20. Expression of the Kdp ATPase Is Consistent with Regulation by Turgor Pressure
The kdpFABC operon of Escherichia coli encodes the four protein subunits of the Kdp K+ transport system. Kdp is expressed when growth is limited by the availability of K+. Expression of Kdp is dependent on the products of the adjacent kdpDE operon, which encodes a pair of two-component regulators. Studies with kdp-lac fusions led to the suggestion that chang
American Society for Microbiology.
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21. Glycine Betaine Transport in Lactococcus lactis Is Osmotically Regulated at the Level of Expression and Translocation Activity
Microorganisms react upon hyperosmotic stress by accumulating compatible solutes. Here we report that Lactococcus lactis uses a transport system for glycine betaine that, contrary to earlier observations (D. Molenaar et al., J. Bacteriol. 175:5438–5444, 1993), is osmotically regulated at the levels of both expression and transport activity.
American Society for Microbiology.
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22. The AtProT Family. Compatible Solute Transporters with Similar Substrate Specificity But Differential Expression Patterns1
Proline transporters (ProTs) mediate transport of the compatible solutes Pro, glycine betaine, and the stress-induced compound γ-aminobutyric acid. A new member of this gene family, AtProT3, was isolated from Arabidopsis (Arabidopsis thaliana), and its properties were compared to AtProT1 and AtProT2. Transient expression of fusions of AtProT and the green f
American Society of Plant Biologists.
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23. Protective Effect of Sucrose and Sodium Chloride for Lactococcus lactis during Sublethal and Lethal High-Pressure Treatments
The bactericidal effect of hydrostatic pressure is reduced when bacteria are suspended in media with high osmolarity. To elucidate mechanisms responsible for the baroprotective effect of ionic and nonionic solutes, Lactococcus lactis was treated with pressures ranging from 200 to 600 MPa in a low-osmolarity buffer or with buffer containing 0.5 M sucrose or 4
American Society for Microbiology.
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24. Role of water in some biological processes.
The state of intracellular water has been a matter of controversy for a long time for two reasons. First, experiments have often given conflicting results. Second, hitherto, there have been no plausible grounds for assuming that intracellular water should be significantly different from bulk water. A collective behavior of water molecules is suggested here a