Combinatorial Library
Mostrando 13-24 de 163 artigos, teses e dissertações.
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13. A síntese orgânica em fase sólida e seus suportes poliméricos mais empregados
In the last decade we have seen improved a powerfull tool to medicinal chemistry: the Solid Phase Organic Synthesis (SPOS). This metodology can be used to synthesize a large library of compounds in a short time by combinatorial chemistry, where simple chemical substances can be combinated one to each other building a library of complex compounds. In this wor
Química Nova. Publicado em: 2001-12
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14. PURIFICATION OF alfa-LACTALBUMIN USING PEPTIDES AS AFFINITY CHROMATOGRAPHY LIGANDS
A method for the purification of alfa-lactalbumin from whey protein isolate solutions using a hexapeptide ligand has been developed. The peptide WHWRKR was isolated from a peptide library generated by combinatorial chemistry, and displays affinity to alfa-lactalbumin. Other proteins from whey protein isolate bound nonspecifically to the resin used. Different
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 2000
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15. Combinatorial library diversity: probability assessment of library populations.
A method is described for measuring the diversity of combinatorial oligonucleotide libraries that entails extrapolating the base composition of a co-synthesized model library (dNC, N = A, C, G, T) to that of a multibase library template. The base composition of dNC was measured by HPLC. The ability of dNC to predict the base composition of a multibase librar
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16. Library of libraries: approach to synthetic combinatorial library design and screening of "pharmacophore" motifs.
Construction of synthetic combinatorial libraries is described that allows for the generation of a library of motifs rather than a library of compounds. Peptide libraries based on this strategy were synthesized and screened with model targets streptavidin and anti-beta-endorphin antibody. The screens resulted in observation of expected motifs providing evide
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17. In vitro selection and affinity maturation of antibodies from a naive combinatorial immunoglobulin library.
We have used a combinatorial immunoglobulin library approach to obtain monoclonal antibodies from nonimmune adult mice, thereby establishing the principles of (i) accessing naive combinatorial antibody libraries for predetermined specificities and (ii) increasing the affinity of the selected antibody binding sites by random mutagenesis. A combinatorial Fab l
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18. Combinatorial approaches: A new tool to search for highly structured β-hairpin peptides
Here we present a combinatorial approach to evolve a stable β-hairpin fold in a linear peptide. Starting with a de novo-designed linear peptide that shows a β-hairpin structure population of around 30%, we selected four positions to build up a combinatorial library of 204 sequences. Deconvolution of the library using circular dichroism reduced such a seque
The National Academy of Sciences.
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19. Recursive deconvolution of combinatorial chemical libraries.
A recursive strategy that solves for the active members of a chemical library is presented. A pentapeptide library with an alphabet of Gly, Leu, Phe, and Tyr (1024 members) was constructed on a solid support by the method of split synthesis. One member of this library (NH2-Tyr-Gly-Gly-Phe-Leu) is a native binder to a beta-endorphin antibody. A variation of t
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20. Mixture-Based Heterocyclic Combinatorial Positional Scanning Libraries: Discovery of Bicyclic Guanidines Having Potent Antifungal Activities against Candida albicans and Cryptococcus neoformans
A mixture-based synthetic combinatorial library of more than 100,000 bicyclic guanidines was generated in a positional scanning format and assayed for activity against Candida albicans. Potent individual bicyclic guanidines were directly identified following the screening of the library. Time-kill curve studies indicated bactericidal activities for the indiv
American Society for Microbiology.
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21. Virtual combinatorial libraries: Dynamic generation of molecular and supramolecular diversity by self-assembly
Molecular and supramolecular diversity may be generated, respectively, by reversible, covalent or noncovalent self-assembly of basic components whose various potential combinations in number and nature represent a virtual combinatorial library. This concept is applied to the induction of inhibitors of carbonic anhydrase (CA) by reversible recombination of al
The National Academy of Sciences of the USA.
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22. Combinatorial autoantibodies to dihydrolipoamide acetyltransferase, the major autoantigen of primary biliary cirrhosis.
mRNA from a regional lymph node of a patient with primary biliary cirrhosis (PBC) was used to construct a combinatorial immunoglobulin library in the lambda phage vector system. Six human monoclonal IgG Fab clones (LC1-LC6) specific for the major autoantigen of PBC--dihydrolipoamide acetyltransferase, the E2 subunit of the pyruvate dehydrogenase complex (PDC
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23. Dendrimer-supported combinatorial chemistry.
A new methodology for the construction of combinatorial libraries is described. The approach, termed dendrimer-supported combinatorial chemistry (DCC), centers on the use of dendrimers as soluble supports. Salient features of DCC include solution phase chemistry, homogeneous purification, routine characterization of intermediates, and high support loadings.
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24. Encoded combinatorial chemistry.
The diversity of chemical synthesis and the power of genetics are linked to provide a powerful, versatile method for drug screening. A process of alternating parallel combinatorial synthesis is used to encode individual members of a large library of chemicals with unique nucleotide sequences. After the chemical entity is bound to a target, the genetic tag ca