Coccidia
Mostrando 25-36 de 41 artigos, teses e dissertações.
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25. Coccidia in Subepithelial Infections of the Intestines of Birds
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26. Identification of bovine Neospora parasites by PCR amplification and specific small-subunit rRNA sequence probe hybridization.
Neospora is a newly recognized genus of pathogenic coccidia, closely related to Toxoplasma gondii, that can cause abortion or congenital disease in a variety of domestic animal hosts. On the basis of the small-subunit rRNA gene sequences of Neospora spp. and other apicomplexa coccidia, oligonucleotide primers COC-1 and COC-2 were used for PCR amplification o
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27. A condition dependent link between testosterone and disease resistance in the house finch.
Testosterone has recently been proposed as a link between male quality and health and the expression of sexual traits. We investigated the relationship between testosterone and measures of the individual condition and health of males in a natural population of house finches (Carpodacus mexicanus). We also conducted a captive experiment in order to test for t
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28. Cross-protection against four species of chicken coccidia with a single recombinant antigen.
A cDNA clone, SO7', from an Eimeria tenella cDNA library was inserted into the high-expression vector pJC264 and was expressed in Escherichia coli as a fusion protein, CheY-SO7', with a molecular mass of approximately 36 kDa. By using the purified recombinant antigen to immunize young chicks, it was demonstrated that a single dose, without adjuvant, not only
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29. Strain-Dependent Differences in Murine Susceptibility to Coccidia †
Differences in susceptibility of strains of mice to Eimeria ferrisi were observed by infecting eight strains of mice with six infectious dose levels and comparing the mortality rate among the strains for a period of 12 days. Mice of the C57BL/6 and HA/ICR strains were susceptible, and those of A/He, AKR, BALB/c, CBA, C3H/Anf, and DBA/2 strains were resistant
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30. A new acid-fast trichrome stain for simultaneous detection of Cryptosporidium parvum and microsporidial species in stool specimens.
The detection in stool specimens of Cryptosporidium parvum and microsporidia, the most frequent parasitic pathogens causing diarrhea in AIDS patients, until now has depended on two different staining methods. However, since double infections occur and minimization of laboratory costs is mandatory, development of a method for simultaneous detection of these p
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31. In vivo expression of in vitro anticoccidial activity.
Large-scale screening has led to the identification of several experimental compounds that have very potent intrinsic activity against coccidia, but the lack of translation to in vivo efficacy has been a major hurdle in developing such leads into effective new drugs. We developed methods to explore the impact of oral availability and appropriate distribution
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32. Demonstration of serum antibodies to Cryptosporidium sp. in normal and immunodeficient humans with confirmed infections.
Antibodies to Cryptosporidium sp. were detected in sera from 12 immunocompetent individuals recovered from cryptosporidiosis and from 5 subjects with an acquired immunodeficiency syndrome and persistent cryptosporidiosis by an indirect immunofluorescent (IIF) test. Marked seroconversion accompanied recovery from infection in immunocompetent individuals, and
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33. RUPTURE OF BACTERIA BY EXPLOSIVE DECOMPRESSION
Foster, John W. (University of Georgia, Athens), Robert M. Cowan, and Ted A. Maag. Rupture of bacteria by explosive decompression. J. Bacteriol. 83:330–334. 1962.—A device is described for instantaneously rupturing bacteria and other cells in a closed system under controlled conditions by explosive decompression. With this device, 31 to 59% of Serratia m
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34. Prevalence of and Risk Factors for Feline Tritrichomonas foetus and Giardia Infection
Data were gathered for 117 cats from 89 catteries at an international cat show to examine prevalence and risk factors for feline Tritrichomonas foetus and Giardia infection. Prevalence of T. foetus was 31% among cats (36 out of 117) and catteries (28 out of 89) based on results of fecal smear examination (5 out of 36), fecal culture in modified Diamond's med
American Society for Microbiology.
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35. Techniques for the recovery and identification of Cryptosporidium oocysts from stool specimens.
Due to increasing numbers of patients with documented infections with Cryptosporidium and other coccidia, it is important for the physician and clinical laboratory to be aware of the appropriate diagnostic techniques necessary for organism recovery and identification. Although Cryptosporidium is found in the gastrointestinal tract, tissue biopsies may be ins
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36. Immunization of chickens with live Escherichia coli expressing Eimeria acervulina merozoite recombinant antigen induces partial protection against coccidiosis.
Inoculation of chickens with live Escherichia coli N6405 transformants containing a plasmid which encodes ampicillin resistance and an immunodominant p250 surface antigen of Eimeria acervulina merozoites induced partial protection against challenge with live coccidia. The inoculation with E. coli transformants induced antigen-specific immunoglobulin and cell