Clostridium Tyrobutyricum
Mostrando 1-10 de 10 artigos, teses e dissertações.
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1. Atividade inibitória de bovicina HC5 sobre bactérias deterioradoras de polpa de manga / Inhibitory activity of bovicin HC5 against spoilage bacteria from mango pulp
Bacteriocins from lactic acid bacteria have been suggested as an alternative to traditional food preservation methods, such as heat treatment, that interfere with natural characteristics of the food. Nisin is the bacteriocin that has been most used in foods. Recently, a new bacteriocin, bovicin HC5, produced by Streptococcus bovis HC5, was characterized. Pre
Publicado em: 2006
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2. Isolation, characterization and fermentative activity of mango pulp spoilage bacteria / Isolamento, caracterização e atividade fermentativa de bactérias deterioradoras de polpa de manga
The mango (Mangifera indica Linn.) is one of the most important tropical fruits. It is very appreciated due to its flavor and nutritional characteristics. The fruits are easily spoiled because of intrinsic factors, which makes difficult its commercialization in natura. An alternative to reduce the losts is the conservation of the mango as pulp, juice and nec
Publicado em: 2006
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3. A direct PCR detection method for Clostridium tyrobutyricum spores in up to 100 milliliters of raw milk.
A direct detection method for Clostridium tyrobutyricum spores in up to 100 ml of raw milk is presented. The bacterial spores are concentrated by centrifugation after chemical extraction of the milk components. The vegetative cells are selectively lysed, and their DNA is digested and washed away. Afterwards, the DNA is liberated from the spores by microwave
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4. Identification of Clostridium tyrobutyricum as the causative agent of late blowing in cheese by species-specific PCR amplification.
Butyric acid fermentation, the late-blowing defect in cheese, caused by the outgrowth of clostridial spores present in raw milk, can create considerable loss of product, especially in the production of semihard cheeses like Gouda cheese, but also in grana and Gruyère cheeses. To demonstrate the causative relationship between Clostridium tyrobutyricum and la
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5. Action of egg white lysozyme on Clostridium tyrobutyricum.
A 500-U ml-1 portion of egg white lysozyme was able to kill 99% of 5 X 10(5) resting vegetative cells of Clostridium tyrobutyricum within 24 h of incubation at 25 degrees C. Spores were completely resistant to lysozyme. Proliferating vegetative cells were severely inhibited, although lysozyme-resistant cells developed in growing cultures in the presence of l
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6. Development and Validation of PCR Primers To Assess the Diversity of Clostridium spp. in Cheese by Temporal Temperature Gradient Gel Electrophoresis
A nested-PCR temporal temperature gradient gel electrophoresis (TTGE) approach was developed for the detection of bacteria belonging to phylogenetic cluster I of the genus Clostridium (the largest clostridial group, which represents 25% of the currently cultured clostridial species) in cheese suspected of late blowing. Primers were designed based on the 16S
American Society for Microbiology.
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7. Identification and Characterization of Two Novel Clostridial Bacteriocins, Circularin A and Closticin 574
Two novel antibacterial peptides of clostridial species were purified, N-terminally sequenced, and characterized. Moreover, their structural genes were identified. Closticin 574 is an 82-amino-acid bacteriocin produced by Clostridium tyrobutyricum ADRIAT 932. The supernatant of the producing strain showed a high level of activity against the indicator strain
American Society for Microbiology.
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8. Gnotobiotic Silage
Selected strains of lactic acid bacteria isolated from grass silage were found to flourish when inoculated into irradiation-sterilized forage under gnotobiotic conditions. The acid content and pH of these silages resembled naturally fermented silage. Inoculation of gnotobiotic silage with Clostridium sporogenes and C. tyrobutyricum failed to cause any notice
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9. Antimicrobial activity of lysozyme against bacteria involved in food spoilage and food-borne disease.
Egg white lysozyme was demonstrated to have antibacterial activity against organisms of concern in food safety, including Listeria monocytogenes and certain strains of Clostridium botulinum. We also found that the food spoilage thermophile Clostridium thermosaccharolyticum was highly susceptible to lysozyme and confirmed that the spoilage organisms Bacillus
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10. Macedocin, a Food-Grade Lantibiotic Produced by Streptococcus macedonicus ACA-DC 198
Streptococcus macedonicus ACA-DC 198, a strain isolated from Greek Kasseri cheese, produces a food-grade lantibiotic named macedocin. Macedocin has a molecular mass of 2,794.76 ± 0.42 Da, as determined by electrospray mass spectrometry. Partial N-terminal sequence analysis revealed 22 amino acid residues that correspond with the amino acid sequence of the l
American Society for Microbiology.