Clostridium Botulinum C E D
Mostrando 13-24 de 41 artigos, teses e dissertações.
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13. Perfil sorológico das amostras de Clostridium botulinum tipos C e D utilizadas para produção de imunógenos no Brasil
Com o objetivo de avaliar o perfil sorológico de três amostras de Clostridium botulinum tipo C e três do tipo D utilizadas para produção de imunógenos no Brasil, determinou-se o índice de eficiência e o grau de homologia sorológica dentro de cada tipo. O índice de eficiência mostrou a mesma tendência para os dois tipos. Os consumos relativos de a
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Publicado em: 2000-04
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14. Distribuição de esporos de clostridium botulinum no solo em torno de cadáveres decompostos de bovinos vítimas de botulismo em pastagens no sul de Goiás
Foi avaliada a distribuição de esporos de Clostridium Botulinum em torno de 30 cadáveres decompostos de bovinos supostamente vítimas de botulismo, de 15 municípios no sul de Goiás. A partir do local em que o cadáver se decompôs e na direção dos quatro pontos cardeais, contaram-se 630 amostras de solo. A detecção de toxina botulínica dos filtrado
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 20/12/1985
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15. Identification of Clostridium botulinum, Clostridium argentinense, and related organisms by cellular fatty acid analysis.
On the basis of 686 analyses of 285 strains of Clostridium botulinum, Clostridium argentinense (formerly C. botulinum type G), and phenotypically related organisms, 14 cellular fatty acid (CFA) groups of toxic organisms and 6 CFA groups of nontoxic organisms were delineated. The CFA groups of toxic strains included two of type A, three of proteolytic strains
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16. Establishment of a monoclonal antibody recognizing an antigenic site common to Clostridium botulinum type B, C1, D, and E toxins and tetanus toxin.
The partial amino acid sequence of the light-chain (Lc) component of Clostridium botulinum type C1 toxin was determined. The sequence was quite similar to those of the other types of botulinum and tetanus toxins. Nine monoclonal antibodies against botulinum type E toxin were established by immunizing BALB/c mice with type E toxoid or its Lc component. Six an
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17. Acid precipitation of Clostridium botulinum type C and D toxins from whole culture by addition of ribonucleic acid as a precipitation aid.
The ratios of ribonucleic acid to protein contents of Clostridium botulinum type C, D, and E cultures were lower than those of type A, B, and F cultures. Addition of ribonucleic acid at 0.4 mg/ml to culture satisfactorily aided acid precipitation of type C and D toxins, but not that of type E toxin.
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18. Cloning of a DNA sequence unique to Clostridium botulinum group I by selective hybridization.
Nucleic acid sequences were isolated from a strain of Clostridium botulinum type A by a selective hybridization method known as deletion enrichment. Nontoxigenic C. sporogenes was used to produce a C. botulinum type A sequence-enriched library. A probe, pCBM44, which showed specific hybridization to a 4.0-kb HindIII fragment present in all of the C. botulinu
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19. Detection of Clostridium botulinum type A toxin by enzyme-linked immunosorbent assay with antibodies produced in immunologically tolerant animals.
Immunological tolerance is a state of unresponsiveness to foreign substances (antigens) which can develop in human and animal species as the result of continued exposure to antigens early in life. We utilized this principle for the preparation of antibodies against Clostridium botulinum type A toxin. By selective suppression of the immunological response of
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20. Clostridium botulinum in the Gulf of Thailand.
A survey was carried out to determine the incidence of Clostridium botulinum in samples of mud, sand, and fish from the Gulf of Thailand. Enrichment cultures from 762 samples of mud and sand from seven different areas around the Gulf were tested. C. botulinum type D was present in 10 samples, and type E was present in 2 samples taken from the west coast at H
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21. Production of C2 toxin by Clostridium botulinum types C and D as determined by its vascular permeability activity.
Vascular permeability (VP) activity was demonstrated by intradermal injection of culture supernatants of Clostridium botulinum types C and D and strains producing only C2 toxin. The activity was enhanced markedly by treatment with trypsin. It was abolished by antiserum against C2 toxin and by antisera specific for components I and II of C2 toxin, but not by
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22. Detection of Clostridium botulinum type G toxin by enzyme-linked immunosorbent assay.
Clostridium botulinum type G toxin was detected and quantified readily with the enzyme-linked immunosorbent assay. With the double-sandwich technique and alkaline phosphatase as the enzyme indicator, C. botulinum toxin type G was detected in quantities equaling those required for one mouse intraperitoneal median lethal dose. The time required for the procedu
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23. SPORULATION OF CLOSTRIDIUM BOTULINUM TYPES A, B, AND E, CLOSTRIDIUM PERFRINGENS, AND PUTREFACTIVE ANAEROBE 3679 IN DIALYSIS SACS1
Schneider, Morris D. (Quartermaster Food and Container Institute for the Armed Forces, U.S. Army, Chicago, Ill.), Nicholas Grecz, and Abe Anellis. Sporulation of Clostridium botulinum types A, B, and E, Clostridium perfringens, and Putrefactive Anaerobe 3679 in dialysis sacs. J. Bacteriol. 85:126–133. 1963.—Concentrated cultures of spores of Clostridium
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24. Recovery of spores of Clostridium botulinum in yeast extract agar and pork infusion agar after heat treatment.
Yeast extract agar, pork infusion agar, and modifications of these media were used to recover heated Clostridium botulinum spores. The D- and z-values were determined. Two type A strains and one type B strain of C. botulinum were studied. In all cases the D-values were largest when the spores were recovered in yeast extract agar, compared to the D-values for