Cloned
Mostrando 1-12 de 16770 artigos, teses e dissertações.
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1. Design and evaluation of an AFLP molecular marker for the detection of Coccidioides spp. in biological samples
ABSTRACT At present, there is no standardized marker that is routinely used in clinical laboratories to diagnose coccidioidomycosis. Thus, the goals of this study were to obtain a sequence characterized amplified region (SCAR) marker for the identification of Coccidioides spp., evaluate its specificity and sensitivity in fungal DNA-spiked blood and sputum sa
Braz J Infect Dis. Publicado em: 25/11/2019
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2. Clinical and blood gas analysis of calves conceived by artificial insemination, in vitro fertilization and animal cloning
RESUMO: Para que ocorra adaptação extra-uterina bem sucedida é necessário que o neonato consiga estabelecer suas funções respiratórias de maneira eficaz, garantindo oxigenação eficiente e boa vitalidade. Distúrbios respiratórios são os maiores causadores de óbito durante o período neonatal em bovinos, e essa mortalidade é ainda mais expressiva
Pesq. Vet. Bras.. Publicado em: 30/09/2019
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3. Identification and Characterisation of Alternative Splice Variants of Hoxb9 and Their Correlation with Melanogenesis in the Black-Boned Chicken
ABSTRACT Homeobox B9 (HOXB9) gene has been demonstrated to be associated with melanogenesis in chicken plumage by high-throughput RNA sequencing. In this study, we cloned and characterised HOXB9 in black-boned chickens. Two alternative splice variants (HOXB9-1 and HOXB9-2) were identified in chicken feather bulbs. Expression analysis of HOXB9 in 11 differen
Braz. J. Poult. Sci.. Publicado em: 19/08/2019
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4. Isolation and characterization of a human cementocyte-like cell line, HCY-23
Abstract Cementum is the mineralized tissue covering the tooth root that functions in tooth attachment and post-eruptive adjustment of tooth position. It has been reported to be highly similar to bone in several respects but remains poorly understood in terms of development and regeneration. Here, we investigate whether cementocytes, the residing cells in ce
Braz. oral res.. Publicado em: 15/08/2019
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5. A remark on quantum cloning
Abstract Physical entities are not truly teleported in so-called quantum teleportation, only their states are. Similarly, physical entities are not truly cloned in so-called quantum cloning, only their states are. The celebrated no-cloning theorem tells us that a universal cloning machine, able to perfectly clone arbitrary states, cannot be built. However, t
Rev. Bras. Ensino Fís.. Publicado em: 18/04/2019
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6. Advances in the spread of vegetative blueberry
Resumo A falta de método eficiente de propagação em algumas cultivares dificulta a expansão da cultura do mirtileiro. No Brasil ainda não se produz muda com qualidade e quantidades necessárias para alavancar o setor. A clonagem do material selecionado através de minipropagação e micropropagação é uma alternativa para aumentar a capacidade de mul
Rev. Bras. Frutic.. Publicado em: 14/03/2019
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7. Expression and Localization of Olfactory Receptor AcerOr1 from Chinese Honey Bee, Apis cerana cerana (Hymenoptera, Apidae)
ABSTRACT Olfactory receptors are essential for recognition and detection of odor in honeybees. Although we have cloned and characterized the sequence of olfactory receptor AcerOr1 before, the tissue distribution and location of this gene in the nurse and the forager worker Apis cerana cerana were not very clear. To further investigate this information of Ace
Braz. arch. biol. technol.. Publicado em: 29/11/2018
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8. Phytoene Synthase Gene ( PSY ) from Sweet Potato ( Ipomoea batatas Lam . ) Enhances Tolerance to Abiotic Stress
ABSTRACT Phytoene synthase (PSY) is the rate-limiting enzyme for carotenoid biosynthesis. To date, several studies focused on PSY genes in the context of abiotic stress responses. In this study, two phytoene synthase encoding genes, IbPSY1 and IbPSY2, were identified from a published transcriptome and bioinformatic analysis showed that they shared conserved
Braz. arch. biol. technol.. Publicado em: 14/11/2018
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9. Cloning and expression of an endoglucanase gene from the thermotolerant fungus Aspergillus fumigatus DBiNU-1 in Kluyveromyces lactis
Abstract An intronless endoglucanase from thermotolerant Aspergillus fumigatus DBINU-1 was cloned, characterized and expressed in the yeast Kluyveromyces lactis. The full-length open reading frame of the endoglucanase gene from A. fumigatus DBiNU-1, designated Cel7, was 1383 nucleotides in length and encoded a protein of 460 amino acid residues. The predicte
Braz. J. Microbiol.. Publicado em: 2018-09
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10. CspB of an arctic bacterium, Polaribacter irgensii KOPRI 22228, confers extraordinary freeze-tolerance
ABSTRACT Freezing temperatures are a major challenge for life at the poles. Decreased membrane fluidity, uninvited secondary structure formation in nucleic acids, and protein cold-denaturation all occur at cold temperatures. Organisms adapted to polar regions possess distinct mechanisms that enable them to survive in extremely cold environments. Among the co
Braz. J. Microbiol.. Publicado em: 2018-03
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11. Molecular Cloning and Mrna Expression Analysis of Sichuan White Goose (Anser Cygnoides) Chrebp Gene
ABSTRACT The carbohydrate response element-binding protein (ChREBP) is an important nuclear factor that regulates glycolysis and de novo lipogenesis. However, the role of ChREBP in fatty liver development in geese remains unclear. In order to understand the function of ChREBP in lipid metabolism of geese, we first cloned the complete cDNA of the ChREBP of th
Rev. Bras. Cienc. Avic.. Publicado em: 2017-12
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12. Expression, Purification and Functional Assessment of Smallest Isoform of Human Interleukin-24 in Escherichia coli
ABSTRACT Interleukin-24 (IL-24) is a novel tumor-suppressor gene that has different alternative splice isoforms. It has been shown that new smallest isoform of human IL-24 gene, lacking three exons, induces higher levels of cytotoxicity than all the isoforms, indicating shortest isoform of IL-24 may be a new promising anti-cancer agent. In this study, we aim
Braz. arch. biol. technol.. Publicado em: 07/08/2017