1. Efficient Expression and Purification of Recombinant Human Enteropeptidase Light Chain in Esherichia coli

Human enterokinase (synonym: enteropeptidase, EC 3.4.21.9) light chain (hEKL) gene was designed and artificially synthesized with built-in codon blas towards Escherichia colicodon preference. The synthetic hEKL gene was cloned into prokaryotic expression vector pMAL-s and transferred into the expression strain E. coli BL21 (DE3). Recombinant hEKL protein wit

Braz. arch. biol. technol.. Publicado em: 14/10/2014

2. Construction and characterization of a bovine herpesvirus 5 mutant with a deletion of the GI, GE and US9 genes

Bovine herpesvirus 5 (BoHV-5) is a important cause of viral encephalitis in cattle in South America. Within the framework of developing a differential vaccine against BoHV-5, a deletion mutant was constructed based on a Brazilian BoHV-5 isolate. The target of the deletions were genes that code proteins implicated in the neurovirulence of BoHV-5, the glycopro

Publicado em: 2010

3. Fisiologia e metabolismo placentário por canulação cordonal em gestações de bovinos normais, FIV e clonados / Placental physiology and metabolism by cordonal canulation in normal, IVF and cloned bovine concepti

O desenvolvimento dos sistemas de produção de embriões por fecundação in vitro (FIV) ou transferência nuclear com células somáticas (TNCS) em diversas espécies animais, em especial a bovina, acarretou na manifestação de anormalidades de desenvolvimento in vivo, com a placenta sendo considerada o fator determinante no aparecimento destes distúrbio

Publicado em: 2010

4. Characterization of recombinant MSP5 Anaplasma marginale Havana isolate

Anaplasma marginale is the causative agent of bovine anaplasmosis, a disease of worldwide economic importance. Major surface proteins (MSPs) are involved in host-pathogen and tick-pathogen interactions and they have been used as markers for the genetic characterization of A. marginale strains and phylogenetic studies. The major surface protein 5 (MSP5) is hi

Brazilian Journal of Microbiology. Publicado em: 2009-12

5. Efeito da pré-maturação sobre o desenvolvimento embrionário de oócitos submetidos à ativação partenogenética e transferência de núcleo / Effect of pre-maturation on embryo development of oocytes submitted to parthenogenetic activation and nuclear transfer

Embryo production rates obtained from both IVF (30-40%) and NT (23%) are still below the expected values. Therefore, oocyte pre-maturation using cell cycle inhibitors is one of the alternatives which has been studied to increase the competence of oocytes used for IVP, as an attempt to optimize the success rates of these biotechniques. Neurotrophins are known

Publicado em: 2009

6. Interação celular na placenta de gestações de bovinos clonados com especial ênfase às integrinas / Cell-cell interactions in the bovine placenta from pregnancies with cloned fetuses with special respect to integrins and their receptors

Integrinas são glicoproteínas transmembrânicas envolvidas na adesão célula-célula e célula-proteina da matriz extracellular (ECM) que participam da migração e fusão de células trofoblásticas gigantes (TGC) com células do epitélio materno no placentoma bovino e interagem com inibidores teciduais de metalloproteinases (TIMPs), considerados import

Publicado em: 2009

7. Patologia de neonatos bovinos clonados / Pathology of cloned bovine neonates

Clonagem ou técnica de transferência nuclear (TN) permite a geração de um organismo geneticamente idêntico a outro. Inúmeras aplicações da TN têm sido descritas para uso em pecuária e em medicina humana. O sucesso na clonagem de ovinos, bovinos, suínos e caprinos confirma a utilidade da TN para o propósito de expansão clonal de animais de valor

Publicado em: 2008

8. Construction of auxotrophic marker in Mycobacterium bovis BCG, knockout strain for the DPPD and proteomic study of tuberculin / Construção de marcador auxotrófico em Mycobacterium bovis BCG, de uma cepa knockout para DPPD e estudo proteômico da tuberculina

Mycobacterium bovis BCG has the potential to be an effective live vector for multivalent vaccines. However, there are two problems regarding the utilization of recombinant BCG as vaccine. The first one is that most mycobacterial cloning vectors rely on antibiotic resistance gene as selectable marker, which is used for genetic transformation. The second one i

Publicado em: 2008

9. Produção de quimosina B de Bos taurus em Pichia pastoris

Enzymatic milk coagulation for cheese manufacturing involves the cleavage of the scissile bond in κ casein by an aspartic protease. Bovine chymosin B is commonly used at the industrial level for milk coagulation because it combines a strong clotting activity with a low general proteolytic activity. In the present study, we report the expression, purific

Publicado em: 2008

10. Efeitos do VEGF e do bFGF sobre a expressão da aromatase P450 em cultivo de células placentárias provenientes de bovinos clonados e não clonados / VEGF and bFGF effects on P450 aromatase expression of cultivated placental cells from cloned and non-cloned bovines

Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) are important regulators of placental development and function. Their effects on the steroidogenic enzyme aromatase P450 (P450arom) expression from bovine placenta at different gestational stages (90 - 270 days) were evaluated by immunocytochemistry. Moreover, we compared the

Publicado em: 2007

11. In silico characterization and epigenetic analysis of in vivo and cloned cattle of the homologue region 11p15.5 involved with Beckwith-Wiedemann syndrome in humans / Caracterização in silico e análise epigenética em bovinos produzidos in vivo e por transferência nuclear da região homóloga à 11p15.5 envolvida com a síndrome de Beckwith-Wiedemann em humanos

Epigenetics is the branch of biology which studies heritable changes in genome function that occur without a change in nucleotide sequence within the DNA. One of the most studied epigenetic process is the DNA methylation, which is associated with several gene regulation mechanisms such as genomic imprinting. This type of regulation is characterized by parent

Publicado em: 2007

12. Passagem celular, sexo e transcrição X-específica interferem no desenvolvimento embrionário e fetal de bovinos produzidos por transferência nuclear / Celular passage, sex and X-specific transcription interfere in bovine nuclear transfer embryo and fetal development

Cells cultured for long-term periods accumulate genetic and epigenetic modifications that result in improper nuclear reprogramming of somatic cell nuclear transfer (SCNT) embryos. Furthermore, the sex may be a limiting factor in blastocysts production and in post-implantation developmental competence. Therefore, the objective of this study was to determine t

Publicado em: 2007