Clamps
Mostrando 13-24 de 107 artigos, teses e dissertações.
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13. Influência da dose de energia sobre o grau de conversão, tensão de polimerização, propriedades mecânicas e microinfiltração in vitro de restaurações em compósito / Influence of the radiant exposure on the degree of conversion, polymerization stress, mechanical properties and in vitro microleakage of composite restorations
Proposition: The aim of this study was to verify the influence of radiant exposure applied to a composite on the degree of conversion (DC), polymerization stress (PS), maximum rate of reaction (Rp max), mechanical properties (flexural strength/FS, elastic modulus/E, Knoop hardness/KHN) and microleakage of in vitro restorations using two adhesive systems. Met
Publicado em: 2007
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14. Laparoscopic surgery for treatment of incisional lumbar hernia
OBJECTIVE: To present results obtained with laparoscopic correction of incisional lumbar hernia in patients with minimum follow-up of 1 year. MATERIALS AND METHODS: We prospectively studied 7 patients diagnosed with incisional lumbar hernia after physical examination and computerized tomography. We used laparoscopic transperitoneal access through 3 ports. On
International braz j urol. Publicado em: 2005-08
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15. Structural and functional similarities of prokaryotic and eukaryotic DNA polymerase sliding clamps.
The remarkable processivity of cellular replicative DNA polymerases derive their tight grip to DNA from a ring-shaped protein that encircles DNA and tethers the polymerase to the chromosome. The crystal structures of prototypical 'sliding clamps' of prokaryotes (beta subunit) and eukaryotes (PCNA) are ring shaped proteins for encircling DNA. Although beta is
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16. Structural and functional similaritites of prokaryotic and eukaryotic DNA polymerase sliding clamps
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17. Distinct roles for ATP binding and hydrolysis at individual subunits of an archaeal clamp loader
Circular clamps are utilised by replicative polymerases to enhance processivity. The topological problem of loading a toroidal clamp onto DNA is overcome by ATP-dependent clamp loader complexes. Different organisms use related protein machines to load clamps, but the mechanisms by which they utilise ATP are surprisingly different. Using mutant clamp loaders
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18. Mechanism of polymerase collision release from sliding clamps on the lagging strand
Replicative polymerases are tethered to DNA by sliding clamps for processive DNA synthesis. Despite attachment to a sliding clamp, the polymerase on the lagging strand must cycle on and off DNA for each Okazaki fragment. In the ‘collision release' model, the lagging strand polymerase collides with the 5′ terminus of an earlier completed fragment, which t
Nature Publishing Group.
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19. Competitive processivity-clamp usage by DNA polymerases during DNA replication and repair
Protein clamps are ubiquitous and essential components of DNA metabolic machineries, where they serve as mobile platforms that interact with a large variety of proteins. In this report we identify residues that are required for binding of the β-clamp to DNA polymerase III of Escherichia coli, a polymerase of the Pol C family. We show that the α polymerase
Oxford University Press.
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20. Ionic currents responsible for the generation of pace-maker current in the rabbit sino-atrial node.
The ionic nature of the pace-maker current (delta Ip, If, Ih) was investigated in rabbit sino-atrial node using a single sucrose-gap voltage-clamp technique. The pace-maker current was activated by hyperpolarizing clamp steps negative to -50 mV and the pace-maker potential was activated by an action potential or a depolarizing clamp step. Neither pace-maker
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21. Glucose effectiveness assessed under dynamic and steady state conditions. Comparability of uptake versus production components.
Glucose tolerance is determined by both insulin action and insulin-independent effects, or "glucose effectiveness," which includes glucose-mediated stimulation of glucose uptake (Rd) and suppression of hepatic glucose output (HGO). Despite its importance to tolerance, controversy surrounds accurate assessment of glucose effectiveness. Furthermore, the relati
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22. Affinity Purification of DNA and RNA from Environmental Samples with Peptide Nucleic Acid Clamps
Bispeptide nucleic acids (bis-PNAs; PNA clamps), PNA oligomers, and DNA oligonucleotides were evaluated as affinity purification reagents for subfemtomolar 16S ribosomal DNA (rDNA) and rRNA targets in soil, sediment, and industrial air filter nucleic acid extracts. Under low-salt hybridization conditions (10 mM NaPO4, 5 mM disodium EDTA, and 0.025% sodium do
American Society for Microbiology.
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23. Effects of fat on glucose uptake and utilization in patients with non-insulin-dependent diabetes.
It was the aim of this study to determine whether FFA inhibit insulin-stimulated whole body glucose uptake and utilization in patients with non-insulin-dependent diabetes. We performed five types of isoglycemic (approximately 11mM) clamps: (a) with insulin; (b) with insulin plus fat/heparin; (c) with insulin plus glycerol; (d) with saline; (e) with saline pl
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24. Equivalence of the insulin sensitivity index in man derived by the minimal model method and the euglycemic glucose clamp.
Studies were done to determine whether the minimal model approach and the glucose clamp measure equivalent indices of insulin action. Euglycemic glucose clamps (glucose, G: 85 mg/dl) were performed at two rates of insulin (I) infusion (15 and 40 mU/min per m2) in 10 subjects (body mass index, BMI, from 21 to 41 kg/m2). Insulin sensitivity index (SI) from cla