Chromosome Painting
Mostrando 13-24 de 61 artigos, teses e dissertações.
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13. The Origin of Human Chromosome 1 and Its Homologs in Placental Mammals
Developing ordered gene maps from multiple mammalian species coupled with chromosome-painting data provide a powerful resource for resolving the evolutionary history of chromosomes and whole genomes. In this work, we recapitulate the evolutionary history of human chromosome 1 and its homologs in placental mammals, putatively the largest physical unit in
Cold Spring Harbor Laboratory Press.
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14. Chromosome painting in plants: in situ hybridization with a DNA probe from a specific microdissected chromosome arm of common wheat.
We report here on the successful painting of a specific plant chromosome within its own genome. Isochromosomes for the long arm of chromosome 5 of the wheat B genome (5BL) were microdissected from first meiotic metaphase spreads of a monoisosomic 5BL line of the common wheat Triticum aestivum cv. Chinese Spring. The dissected isochromosomes were amplified by
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15. Medical genetics: advances in brief: Complete characterization of a large marker chromosome by reverse and forward chromosome painting
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16. Pseudodicentric chromosome 18 diagnosed by chromosome painting and primed in situ labelling (PRINS).
We report on a newborn white male infant with marked dysmorphic features and various congenital malformations. The initial clinical evaluation showed Crouzon-like features as well as some features of trisomy 18 syndrome and trisomy 13 syndrome. The results from conventional cytogenetic analysis showed a structurally abnormal chromosome replacing one normal c
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17. Reverse chromosome painting: a method for the rapid analysis of aberrant chromosomes in clinical cytogenetics.
We describe a method, termed reverse chromosome painting, which allows the rapid analysis of the content and breakpoints of aberrant chromosomes. The method involves the sorting of small numbers of the aberrant chromosome from short term blood culture preparations or cell lines by using bivariate flow karyotype analysis. The sorted chromosomes are amplified
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18. Painting of fourth, a chromosome-specific protein in Drosophila
Chromosome-specific gene regulation is known thus far only as a mechanism to equalize the transcriptional activity of the single male X chromosome with that of the two female X chromosomes. In Drosophila melanogaster, a complex including the five Male-Specific Lethal (MSL) proteins, “paints” the male X chromosome, mediating its hypertranscription. H
The National Academy of Sciences.
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19. Chromosome in situ suppression hybridisation in human male meiosis.
Chromosome in situ suppression hybridisation with biotinylated whole chromosome libraries permits the unequivocable identification of specific human somatic chromosomes in numerous situations. We have now used this so called 'chromosome painting' technique in meiotically dividing cells, isolated from human testicular biopsy. It is shown that the method allow
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20. Reciprocal chromosome painting among human, aardvark, and elephant (superorder Afrotheria) reveals the likely eutherian ancestral karyotype
The Afrotheria, a supraordinal grouping of mammals whose radiation is rooted in Africa, is strongly supported by DNA sequence data but not by their disparate anatomical features. We have used flow-sorted human, aardvark, and African elephant chromosome painting probes and applied reciprocal painting schemes to representatives of two of the Afrotherian orders
The National Academy of Sciences.
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21. Differentially Painting Human Chromosome Arms with Combined Binary Ratio-labeling Fluorescence In Situ Hybridization
Recently we developed a novel strategy for differentially painting all 24 human chromosomes. It is termed COBRA–FISH, short for combined binary ratio labeling–fluorescence in situ hybridization. COBRA–FISH is distinct from the pure combinatorial approach in that only 4 instead of 5 fluorophores are needed to achieve color discrimination of 24 targets.
Cold Spring Harbor Laboratory Press.
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22. Painting of fourth in genus Drosophila suggests autosome-specific gene regulation
Painting of fourth (POF) is a chromosome-specific protein in Drosophila and represents the first example of an autosome-specific protein. POF binds to chromosome 4 in Drosophila melanogaster, initiating at the proximal region, followed by a spreading dependent on chromosome 4-specific sequences or structures. Chromosome-specific gene regulation is known thus
National Academy of Sciences.
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23. Chromosome in situ suppression hybridisation in clinical cytogenetics.
The use of chromosome in situ suppression hybridisation with whole chromosome libraries has previously been reported by various research laboratories to be an effective method of identifying specific human chromosomal material. As a clinical cytogenetic service laboratory we have used the technique as a complement to diagnosis by classical chromosome banding
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24. Molecular cytogenetic dissection of human chromosomes 3 and 21 evolution
Chromosome painting in placental mammalians illustrates that genome evolution is marked by chromosomal synteny conservation and that the association of chromosomes 3 and 21 may be the largest widely conserved syntenic block known for mammals. We studied intrachromosomal rearrangements of the syntenic block 3/21 by using probes derived from chromosomal subreg
The National Academy of Sciences.