Chromosome Painting
Mostrando 1-12 de 61 artigos, teses e dissertações.
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1. Chromosomal polymorphism and comparative chromosome painting in the rufous-collared sparrow (Zonotrichia capensis)
Abstract Zonotrichia capensis is widely distributed in the Neotropics. Previous cytogenetic studies demonstrated the presence of polymorphisms in two chromosome pairs (ZCA2 and ZCA4). Here, we report results based on comparative chromosome painting, using probes derived from Gallus gallus and Leucopternis albicollis, focused on characterizing the chromosome
Genet. Mol. Biol.. Publicado em: 14/11/2018
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2. A remarkable autosomal heteromorphism in Pseudoryzomys simplex 2n = 56; FN = 54-55 (Rodentia, Sigmodontinae)
Pseudoryzomys simplex, the false rice rat, is a monotypic genus of the Oryzomyini tribe (Sigmodontinae) distributed in part of Bolivia, Paraguay, Argentina and Brazil. Its diploid number has been described as 56 acrocentric chromosomes decreasing in size and no karyotype figure has been depicted. Herein, we present karyotypic data on P. simplex, including ch
Genet. Mol. Biol.. Publicado em: 2013
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3. Mammalian chromosome evolution: comparative studies by chromosome painting on two sloth species of Bradypodidae family and two marsupial species / Evolução cromossômica em mamíferos: estudos comparativos por pintura cromossômica em duas espécies de preguiças da família Bradypodidae e em duas espécies de marsupiais da família Didelphidae
In an attempt to shed additional light on mammalian karyotype evolution, we studied, by chromosome painting, the chromosomes of species from two mammalian basal groups, sloths and marsupials. We compared human chromosomes with the chromosomes of two species of three-toed sloths, Bradypus torquatus and Bradypus variegatus, establishing homologies. Analyzing t
Publicado em: 2009
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4. Estudos de citogenética e de filogenia molecular em roedores da tribo Akodontini / Cytogenetics and molecular phylogenetics in rodents of the tribe Akodontini
Traditionally comparative cytogenetic studies are based mainly on banding patterns. Nevertheless, when dealing with species with highly rearranged genomes, as in Akodon species, or with other highly divergent species, cytogenetic comparisons of banding patterns prove to be inadequate. Hence, comparative chromosome painting has become the method of choice for
Publicado em: 2009
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5. Chromosome evolution: Karyotype variability in Platyrrhini and studies of sinteny and homologies between human chromosomes / Evolução cromossômica: estudo da variabilidade cariotípica em Platyrrhini e das homeologias e sintenias com cromossomos humanos
We studied the karyotypes of Brazilian monkeys (Platyrrhini, Primates) using both traditional cytogenetic techniques as well as FISH. FISH analysis employed human probes for chromosome 14, 15 and the X chromosome and a probe of the Y chromosome of Brachyteles arachnoides obtained by chromosome microdissection. Twenty-four individuals of Alouatta guariba clam
Publicado em: 2008
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6. Estudo CromossÃmico em Morcegos HematÃfagos: Zoo-FISH com Sondas CromossÃmicas de Carollia brevicauda e Phyllostomus hastatus (Phyllostomidae -Chiroptera)
A famÃlia Phyllostomidae constitui a terceira maior dentre os Chiroptera, sendo caracterizada por exibir mais variaÃÃes morfolÃgicas do que qualquer outro grupo de mamÃferos, o que gera considerÃvel discordÃncia no estabelecimento de suas relaÃÃes sistemÃticas e evolutivas. Citogeneticamente à caracterizada por cariÃtipos conservados entre espÃc
Publicado em: 2008
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7. Origem e diferenciação do sistema de cromossomos sexuais ZZ/ZW em Triportheus (Characiformes, Characidae): citogenética, mapeamento dos genes ribossomais e microdissecção cromossômica
Chromosomes of distinct species of Triportheus (Characiformes, Characidae) from different hydrographic basins in Brazil, as well as in Argentina, were analyzed in the present work. Triportheus nematurus, from Piracicaba River (São Paulo State, Brazil), was analyzed for the first time, using conventional cytogenetic techniques, base-specific fluorochromes an
Publicado em: 2007
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8. Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas
Head and neck carcinomas represent the sixth most frequent type of cancer in the world, and 90% are derived from squamous cells (HNSCC). In this study of 15 HNSCC cases, extensive aneuploidy was detected by G banding in most tumors. The most frequently observed numerical changes involved gain of a chromosome 22, and loss of chromosomes Y, 10, 17, and 19. The
Genetics and Molecular Biology. Publicado em: 2003
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9. Development of a bovine X chromosome linkage group and painting probes to assess cattle, sheep, and goat X chromosome segment homologies.
The X chromosome linkage group is conserved in placental mammals. However, X chromosome morphological differences, due to internal chromosome rearrangements, exist among mammalian species. We have developed bovine chromosome painting probes for Xp and Xq to assess segment homologies between the submetacentric bovine X chromosome and the acrocentric sheep and
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10. Value of chromosome painting in determining the chromosomal outcome in offspring of a 12;16 translocation carrier.
We currently use direct and reverse chromosome painting in prenatal diagnosis. In a family with a subtle 12;16 translocation, adjacent 1 segregation was diagnosed in the first child, a boy, in whom symptoms compatible with partial trisomy 16p and partial monosomy 12q were seen. In the next pregnancy, a chorionic villus biopsy was tested using chromosome pain
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11. Kallmann syndrome in a boy with a t(1;10) translocation detected by reverse chromosome painting.
Prometaphase chromosomes from a 16 year old boy with hypogonadotrophic hypogonadism and anosmia (Kallmann syndrome) showed a tiny chromosome fragment attached to the long arm of one chromosome 1 without a visible reciprocal translocation chromosome. Chromosome painting with libraries from chromosomes 1 and X excluded a t(X;1) translocation, but failed to det
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12. Elucidation of structural abnormalities of the X chromosome using fluorescence in situ hybridisation with a Y chromosome painting probe.
Particular regions of the X and Y chromosomes share DNA sequence homology to the extent that cross hybridisation occurs. Thus, chromosome painting with a whole Y chromosome probe consistently results in fluorescence on specific regions of the X chromosome as well as the complete Y chromosome. This phenomenon has been exploited to elucidate the structure of u