Cholera Toxin Subunit B
Mostrando 1-12 de 233 artigos, teses e dissertações.
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1. Parenteral adjuvant potential of recombinant B subunit of Escherichia coli heat-labile enterotoxin
BACKGROUND The B subunit of Escherichia coli heat-labile enterotoxin (LTB) is a potent mucosal immune adjuvant. However, there is little information about LTB's potential as a parenteral adjuvant. OBJECTIVES We aimed at evaluating and better understanding rLTB's potential as a parenteral adjuvant using the fused R1 repeat of Mycoplasma hyopneumoniae P97 ad
Mem. Inst. Oswaldo Cruz. Publicado em: 2017-12
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2. Centros subcorticais dos sistemas visual primário e óptico acessório no mocó (kerodon rupestris): caracterização pela projeção retiniana e citoarquitetura.
The primary and accessory optic systems comprise two set of retinorecipient neural clusters. In this study, these visual related centers in the rock cavy were evaluated by using the retinal innervations pattern and Nissl staining cytoarchigtecture. After unilateral intraocular injection of cholera toxin B subunit and immunohistochemical reaction of coronal a
Publicado em: 2009
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3. Development and characterization of an anti-schistosomiasis vaccine composed by the Sm14 protein, from Schistosoma mansoni, using the B subunit of cholera toxin (CTB) as adjuvant / Desenvolvimento e caracterização de uma vacina antiesquistossomose composta pela proteína Sm14 de Schistosoma mansoni utilizando a subunidade B da toxina colérica (CTB) com adjuvante
Introdução: o desenvolvimento de uma vacina contra a esquistossomose será um importante avanço no controle desta doença crônica e muitas vezes debilitante, afetando milhões de pessoas em todo o mundo. Neste trabalho, descrevemos o uso da subunidade B da toxina colérica (CTB) geneticamente fusionada com Sm14 - uma proteína ligadora de ácidos graxos
Publicado em: 2009
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4. Cytoarchitectural and immunohistochemical analysis of the visual system of tuffed capuchin (Cebus apella). / Análise citoarquitetônica e imunoistoquímica de estruturas do sistema visual de macacos-prego (Cebus apella)
O estudo do sistema visual de macacos-prego representa importante questão devido ao aspecto evolutivo que a espécie apresenta. Foram utilizados cinco macacos-prego, 2 kg. Foi efetuda injeção intra-ocular de 100 ml de solução aquosa de toxina colérica subunidade B (CTb) a 1%, sendo a perfusão realizada 15 dias após a injeção int
Publicado em: 2008
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5. A Zona Incerta no sagüi (Callithrix jacchus): Análise Citoarquitetônica, Neuroquímica e Projeção Retiniana
The retinal projections in mammals usually reach, classically, three major functional systems: the primary visual system, the accessory optic system, and the circadian timing system. But the retinal projections also reach areas classically considered non-visual, one of which groups the neurons of the zona incerta (ZI), target this study. The primary visual s
Publicado em: 2008
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6. "O núcleo supraquiasmático e o folheto intergeniculado do mocó (Kerodon rupestris): Projeções retinianas e caracterização imuno-histoquímica
In this study, two circadian related centres, the suprachiasmatic nucleus (SCN) and the intergeniculate leaflet (IGL) were evaluated in respect to their cytoarchitecture, retinal afferents and chemical content of major cells and axon terminals with a tract tracer and immunohistochemical techniques in the rock cavy (Kerodon rupestris), a Brazilian caviidae ro
Publicado em: 2007
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7. Afferent connections of the amygdalopiriform transition area (APir) of the rat. / Conexões aferentes da área de transição amígdalo-piriforme (APir) no rato.
The amygdalo-piriform transition area (APir) lies at the junction of the piriform, periamygdaloid and entorhinal cortices. The afferent connections of this olfactory district were studied with retrograde tracing methods using the cholera toxin B subunit and Fluoro-Gold as tracers. Our retrograde experiments showed that the main input sources to APir derive f
Publicado em: 1999
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8. Antigenic Specificity of Neutralizing Antibody to Cholera Toxin
Selected rabbit antisera to cholera toxin antigens and convalescent cholera patient sera were analyzed using the permeability factor neutralization test and two sensitive in vitro serological assays specific for cholera toxin, cholera toxin A subunit, and cholera toxin B subunit. The results indicated that antisera to cholera toxin contained toxin-neutralizi
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9. Isolation of hybridoma cell lines and characterization of monoclonal antibodies against cholera enterotoxin and its subunits.
Hybridoma cell lines which produced monoclonal antibodies against cholera toxin were isolated. These cell lines were detected with an enzyme-linked immunosorbent assay screening procedure with purified cholera toxin or subunit A of cholera toxin. Seven cell lines were characterized with respect to their reactivity with cholera toxin subunits by Western blot
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10. Use of automated sequencing of polymerase chain reaction-generated amplicons to identify three types of cholera toxin subunit B in Vibrio cholerae O1 strains.
Cholera toxin is the principal factor causing the profuse intestinal fluid secretion that is characteristic of cholera. The DNA sequences of the cholera toxin subunit B structural genes from 45 Vibrio cholerae O1 strains isolated in 29 countries over a period of 70 years were determined by automated DNA sequencing of polymerase chain reaction-generated ampli
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11. Radioimmunoassay for the Antigenic Determinants of Cholera Toxin and Its Components
A radioimmunoassay procedure is described for the detection of cholera toxin and its component polypeptide chains. Cholera toxin, A subunit, B subunit, α chain, and γ chain were iodinated by the chloramine T procedure. Radiolabeling did not significantly alter the polyacrylamide electrophoretic migration patterns of the toxin or its components. Moreover, r
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12. Antibodies against synthetic peptides of the B subunit of cholera toxin: crossreaction and neutralization of the toxin.
Six peptides corresponding to various segments of the B subunit of cholera toxin have been synthesized and covalently linked to tetanus toxoid. Of the antibodies raised in rabbits against these conjugates, four crossreacted to different extents with the intact B subunit and whole native cholera toxin. Antisera to the peptide of sequence 75-85 were not crossr